BRENDA - Enzyme Database

Cdc42 and ARP2/3-independent regulation of filopodia by an integral membrane lipid-phosphatase-related protein

Sigal, Y.J.; Quintero, O.A.; Cheney, R.E.; Morris, A.J.; J. Cell Sci. 120, 340-352 (2007)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.3.4
additional information
regulation of filopodia by LPR1 is not mediated by cdc42 or Rif, and is independent of the Arp2/3 complex
Homo sapiens
Cloned(Commentary)
EC Number
Commentary
Organism
3.1.3.4
LPP1 and LPR1 expressed in Sf9 cells using a baculovirus vector. Recombinant LPR1 and mutants incorporating a C-terminal EGFP tag expressed in HeLa and COS7 cells. Overexpression of EGFP-LPR1 in SK-OV-3 cells
Homo sapiens
Engineering
EC Number
Amino acid exchange
Commentary
Organism
3.1.3.4
additional information
LPR1 variants containing non-conservative substitutions of Ser198 and His200 within the C2 motif and Arg246 from the C3 motif are strongly expressed in transient transfection experiments but fail to induce the formation of filopodia in HeLa cells. In contrast to wild-type LPR1, these mutant LPR1 proteins are exclusively localized to intracellular membranes. LPR1 C-term DELTA43 mutant, is incapable of generating new filopodia, but rather localizes to pre-existing endogenous protrusions extended by these cells. Subcellular distribution of mutant LPR1 C-term DELTA43 between the plasma membrane and intracellular membranes is highly comparable with that of wild-type LPR1
Homo sapiens
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.3.4
RNAi2
reduces expression levels of LPR1 by ca. 60% in SK-OV-3 cells
Homo sapiens
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.3.4
plasma membrane
LPR1 is predominantly distributed uniformly along shafts of protrusions, with an occasional enrichment at their tips. LPR1 may be an integral membrane protein link between the actin core and the surrounding lipid layer of a nascent filopodium. LPP3 does not show these types of membrane protrusions
Homo sapiens
5886
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.3.4
35000
-
LPR1, Western blot analysis
Homo sapiens
3.1.3.4
38000
-
LPR1, Western blot analysis
Homo sapiens
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.3.4
Homo sapiens
Q8TBJ4
LPR1
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.3.4
filopodium
-
Homo sapiens
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.3.4
ceramide 1-phosphate + H2O
LPP1 but not LPR1
681013
Homo sapiens
ceramide + phosphate
-
-
-
?
3.1.3.4
lyso-phosphatidic acid + H2O
LPP1 but not LPR1
681013
Homo sapiens
monoacylglycerol + phosphate
-
-
-
?
3.1.3.4
additional information
LPR1 does not hydrolyze phospholipid substrates under conditions that readily support LPP1 activity
681013
Homo sapiens
?
-
-
-
-
3.1.3.4
phosphatidic acid + H2O
LPP1 but not LPR1
681013
Homo sapiens
diacylglycerol + phosphate
-
-
-
?
3.1.3.4
sphingosine 1-phosphate + H2O
LPP1 but not LPR1
681013
Homo sapiens
sphingosine + phosphate
-
-
-
?
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.3.4
additional information
regulation of filopodia by LPR1 is not mediated by cdc42 or Rif, and is independent of the Arp2/3 complex
Homo sapiens
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.3.4
LPP1 and LPR1 expressed in Sf9 cells using a baculovirus vector. Recombinant LPR1 and mutants incorporating a C-terminal EGFP tag expressed in HeLa and COS7 cells. Overexpression of EGFP-LPR1 in SK-OV-3 cells
Homo sapiens
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
3.1.3.4
additional information
LPR1 variants containing non-conservative substitutions of Ser198 and His200 within the C2 motif and Arg246 from the C3 motif are strongly expressed in transient transfection experiments but fail to induce the formation of filopodia in HeLa cells. In contrast to wild-type LPR1, these mutant LPR1 proteins are exclusively localized to intracellular membranes. LPR1 C-term DELTA43 mutant, is incapable of generating new filopodia, but rather localizes to pre-existing endogenous protrusions extended by these cells. Subcellular distribution of mutant LPR1 C-term DELTA43 between the plasma membrane and intracellular membranes is highly comparable with that of wild-type LPR1
Homo sapiens
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.3.4
RNAi2
reduces expression levels of LPR1 by ca. 60% in SK-OV-3 cells
Homo sapiens
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.3.4
plasma membrane
LPR1 is predominantly distributed uniformly along shafts of protrusions, with an occasional enrichment at their tips. LPR1 may be an integral membrane protein link between the actin core and the surrounding lipid layer of a nascent filopodium. LPP3 does not show these types of membrane protrusions
Homo sapiens
5886
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.3.4
35000
-
LPR1, Western blot analysis
Homo sapiens
3.1.3.4
38000
-
LPR1, Western blot analysis
Homo sapiens
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.3.4
filopodium
-
Homo sapiens
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.3.4
ceramide 1-phosphate + H2O
LPP1 but not LPR1
681013
Homo sapiens
ceramide + phosphate
-
-
-
?
3.1.3.4
lyso-phosphatidic acid + H2O
LPP1 but not LPR1
681013
Homo sapiens
monoacylglycerol + phosphate
-
-
-
?
3.1.3.4
additional information
LPR1 does not hydrolyze phospholipid substrates under conditions that readily support LPP1 activity
681013
Homo sapiens
?
-
-
-
-
3.1.3.4
phosphatidic acid + H2O
LPP1 but not LPR1
681013
Homo sapiens
diacylglycerol + phosphate
-
-
-
?
3.1.3.4
sphingosine 1-phosphate + H2O
LPP1 but not LPR1
681013
Homo sapiens
sphingosine + phosphate
-
-
-
?