Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Kolar, N.W.; Swart, A.C.; Mason, J.I.; Swart, P.
    Functional expression and characterisation of human cytochrome P45017alpha in Pichia pastoris (2007), J. Biotechnol., 129, 635-644.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.19 functional expression of cytochrome P45017alpha in Pichia pastoris strain GS115, subcloning in Escherichia coli strain JM109 Homo sapiens
1.14.14.32 expressed in Pichia pastoris Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.14.19 membrane bound Homo sapiens 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.14.19 Fe2+
-
 Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.19 2 progesterone + 2 AH2 + 2 O2 Homo sapiens
-
 17alpha-hydroxyprogesterone + 16alpha-hydroxyprogesterone + 2 A + 2 H2O
-
 ?
1.14.14.19 additional information Homo sapiens the enzyme is is essential for the maintenance of normal male and female sexual characteristics, as well as the stress response and mineral balance in all mammals ?
-
 ?
1.14.14.19 pregnenolone + AH2 + O2 Homo sapiens
-
 17alpha-hydroxypregnenolone + A + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.19 Homo sapiens
-
-
-
1.14.14.32 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.14.19 recombinant enzyme partially from Pichia pastoris strain GS115 by microsome preparation Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.14.19 adrenal gland
-
 Homo sapiens
-
1.14.14.19 gonad
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.19 2 progesterone + 2 AH2 + 2 O2
-
 Homo sapiens 17alpha-hydroxyprogesterone + 16alpha-hydroxyprogesterone + 2 A + 2 H2O
-
 ?
1.14.14.19 additional information the enzyme is is essential for the maintenance of normal male and female sexual characteristics, as well as the stress response and mineral balance in all mammals Homo sapiens ?
-
 ?
1.14.14.19 additional information the enzyme catalyzes both the 17alpha-hydroxylation of C21-steroids, pregnenolone and progesterone, and the subsequent 17,20-lyase reaction, EC 4.1.2.30, cleaving the C17-C20 bond of 17alpha-hydroxylated intermediates, 17alpha-hydroxyprenenolone and 17alpha-hydroxyprogesterone to yield C19 androgens, dehydroepiandrosterone and androstenedione, respectively, overview Homo sapiens ?
-
 ?
1.14.14.19 pregnenolone + AH2 + O2
-
 Homo sapiens 17alpha-hydroxypregnenolone + A + H2O
-
 ?
1.14.14.32 17alpha-hydroxyprogesterone + [reduced NADPH-hemoprotein reductase] + O2
-
 Homo sapiens androstenedione + acetate + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.14.19 CYP17
-
 Homo sapiens
1.14.14.19 cytochrome P45017alpha
-
 Homo sapiens
1.14.14.32 C17,20 lyase
-
 Homo sapiens
1.14.14.32 CYP17 catalyzes both steroid 17-hydroxylation and C17,20 lyase reactions Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.14.19 cytochrome P450
-
 Homo sapiens
1.14.14.19 heme
-
 Homo sapiens