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Literature summary extracted from
- Functional characterization and evolutionary implication of the internal 157-amino-acid sequence of an exoinulinase from Penicillium sp. strain TN-88 (2007), J. Biosci. Bioeng., 103, 293-297.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.2.1.80 | the internal region D' encoding the 157-amino-acid sequence in the Penicillium exoinulinase gene inuD cDNA is inserted into the site between the nucleotides 897 and 898 of the Aspergillus niger exoinulinase gene inuE cDNA. The resultant inuE:D' fusion is expressed in Pichia pastoris | Penicillium sp. |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 3.2.1.80 | additional information | the internal region D' encoding the 157-amino-acid sequence in the Penicillium exoinulinase gene inuD cDNA is inserted into the site between the nucleotides 897 and 898 of the Aspergillus niger exoinulinase gene inuE cDNA. The resultant inuE:D' fusion is expressed in Pichia pastoris. The Km-value of the secreted hybrid enzyme InuE:D' for inulin hydrolysis is about 1/15 that of the Aspergillus niger InuE, whereas its kcat value does not differ greatly from that of the InuE | Penicillium sp. |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.80 | 0.22 | - |
inulin | InuE:D' hybrid | Penicillium sp. |  |
| 3.2.1.80 | 3 | - |
inulin | InuE | Penicillium sp. | |
| 3.2.1.80 | 48.7 | - |
sucrose | InuE:D' hybrid | Penicillium sp. | |
| 3.2.1.80 | 111 | - |
sucrose | InuE | Penicillium sp. | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.2.1.80 | extracellular | - |
Penicillium sp. | - |
- |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.80 | 73924 | - |
x * 73924, InuE:D hybrid, calculated from sequence | Penicillium sp. |
| 3.2.1.80 | 110000 | - |
x * 110000, InuE:D hybrid, SDS-PAGE | Penicillium sp. |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.80 | Penicillium sp. | - |
- |
- |
| 3.2.1.80 | Penicillium sp. TN-88 | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.80 | purification of recombinant inuE and inuE:D' fusion protein. The internal region D' encoding the 157-amino-acid sequence in the Penicillium exoinulinase gene inuD cDNA is inserted into the site between the nucleotides 897 and 898 of the Aspergillus niger exoinulinase gene inuE cDNA. The resultant inuE:D' fusion is expressed in Pichia pastoris and purified | Penicillium sp. |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.80 | 40.4 | - |
substrate: inulin, recombinant InuE | Penicillium sp. |
| 3.2.1.80 | 150 | - |
substrate: sucrose, recombinant InuE | Penicillium sp. |
| 3.2.1.80 | 171 | - |
substrate: sucrose, InuE:D' hybrid | Penicillium sp. |
| 3.2.1.80 | 280 | - |
substrate: inulin,InuE:D' hybrid | Penicillium sp. |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.80 | inulin + H2O | - |
Penicillium sp. | D-fructose + ? | - |
? | |
| 3.2.1.80 | inulin + H2O | - |
Penicillium sp. TN-88 | D-fructose + ? | - |
? | |
| 3.2.1.80 | sucrose + H2O | - |
Penicillium sp. | D-fructose + D-glucose | - |
? | |
| 3.2.1.80 | sucrose + H2O | - |
Penicillium sp. TN-88 | D-fructose + D-glucose | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.2.1.80 | ? | x * 110000, InuE:D' hybrid, SDS-PAGE | Penicillium sp. |
| 3.2.1.80 | ? | x * 73924, InuE:D' hybrid, calculated from sequence | Penicillium sp. |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.1.80 | inuE | - |
Penicillium sp. |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.80 | 55 | - |
substrate: inulin, InuE:D' hybrid | Penicillium sp. |
| 3.2.1.80 | 60 | - |
substrate: inulin or sucrose, recombinant enzyme InuE | Penicillium sp. |
| 3.2.1.80 | 60 | - |
substrate: sucrose, InuE:D' hybrid | Penicillium sp. |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.80 | 45 | - |
30 min, inulinase activity of InuE:D' is stable below | Penicillium sp. |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.80 | 500 | - |
inulin | InuE | Penicillium sp. | |
| 3.2.1.80 | 1110 | - |
inulin | InuE:D' hybrid | Penicillium sp. | |
| 3.2.1.80 | 3330 | - |
sucrose | InuE:D' hybrid | Penicillium sp. | |
| 3.2.1.80 | 5000 | - |
sucrose | InuE | Penicillium sp. | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.80 | 4 | - |
substrate: inulin or sucrose, recombinant enzyme InuE | Penicillium sp. |
| 3.2.1.80 | 4.5 | - |
substrate: inulin and sucrose, InuE:D' hybrid | Penicillium sp. |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.80 | 4.5 | 10 | 30°C, 24 h, InuE:D', stable | Penicillium sp. |
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