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Literature summary extracted from

  • Oka, T.; Nemoto, T.; Jigami, Y.
    Functional analysis of Arabidopsis thaliana RHM2/MUM4, a multidomain protein involved in UDP-D-glucose to UDP-L-rhamnose conversion (2007), J. Biol. Chem., 282, 5389-5403.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.76 expression in Saccharomyces cerevisae Arabidopsis thaliana
4.2.1.76 expression in Saccharomyces cerevisae. Recombinant His6-tagged RHM2-N protein (amino acids 1-370). The RHM2/MUM4 protein is more stable than RHM1 or RHM3 in yeast cells Arabidopsis thaliana

Protein Variants

EC Number Protein Variants Comment Organism
4.2.1.76 D96N mutation completely abolishes UDP-Glc 4,6-dehydratase activity Arabidopsis thaliana
4.2.1.76 G18A mutation completely abolishes UDP-Glc 4,6-dehydratase activity Arabidopsis thaliana
4.2.1.76 G193R mutation completely abolishes UDP-Glc 4,6-dehydratase activity Arabidopsis thaliana
4.2.1.76 K165A mutation completely abolishes UDP-Glc 4,6-dehydratase activity Arabidopsis thaliana
4.2.1.76 K35A activity of the mutant is lower than activity of wild-type enzyme Arabidopsis thaliana

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.76 UDP 1 mM, 41% inhibition of purified His6-tagged RHM2-N protein is inhibited Arabidopsis thaliana
4.2.1.76 UDP-Rha 1 mM, 82% inhibition of purified His6-tagged RHM2-N protein Arabidopsis thaliana
4.2.1.76 UDP-Xyl 1 mM, 79% inhibition of purified His6-tagged RHM2-N protein is inhibited Arabidopsis thaliana
4.2.1.76 UMP 1 mM, 23% inhibition of purified His6-tagged RHM2-N protein is inhibited Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.76 0.116
-
 UDP-D-glucose recombinant RHM2-N protein (amino acids 1–370) Arabidopsis thaliana

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.76 additional information
-
 RHM2/MUM4 is a trifunctional enzyme with UDP-D-glucose 4,6-dehydratase, UDP-4-keto-6-deoxy-D-glucose 3,5-epimerase, and UDP-4-keto-L-rhamnose 4-keto-reductase activities. The N-terminal region of RHM2 (RHM2-N: amino acids 1-370) has the first activity and the C-terminal region of RHM2 (RHM2-C: amino acids 371-667) has the two following activities Arabidopsis thaliana

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.76 Arabidopsis thaliana
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.76 UDP-D-glucose
-
 Arabidopsis thaliana UDP-4-dehydro-6-deoxy-D-glucose
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.1.76 RHM1 trifunctional enzyme with UDP-D-glucose 4,6-dehydratase, UDP-4-keto-6-deoxy-D-glucose 3,5-epimerase, and UDP-4-keto-L-rhamnose 4-keto-reductase activities Arabidopsis thaliana
4.2.1.76 RHM2/MUM4 trifunctional enzyme with UDP-D-glucose 4,6-dehydratase, UDP-4-keto-6-deoxy-D-glucose 3,5-epimerase, and UDP-4-keto-L-rhamnose 4-keto-reductase activities. The N-terminal region of RHM2 (RHM2-N, amino acids 1-370) has the first activity and the C-terminal region of RHM2 (RHM2-C, amino acids 371-667) has the two following activities Arabidopsis thaliana
4.2.1.76 RHM3 trifunctional enzyme with UDP-D-glucose 4,6-dehydratase, UDP-4-keto-6-deoxy-D-glucose 3,5-epimerase, and UDP-4-keto-L-rhamnose 4-keto-reductase activities Arabidopsis thaliana
4.2.1.76 UDP-Glc 4,6-dehydratase
-
 Arabidopsis thaliana

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.1.76 35
-
-
 Arabidopsis thaliana

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4.2.1.76 25 45 25°C: about 50% of maximal activity, 45°C: about 40% of maximal activity Arabidopsis thaliana

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.1.76 2860
-
 UDP-D-glucose recombinant RHM2-N protein (amino acids 1–370) Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.76 7.5
-
 the highest level of enzyme activity is observed in 250 mM MOPS-NaOH buffer, pH 7.5. In 250 mM Tris-HCl buffer, pH 7.5, the relative activity is lower than that observed for MOPS-NaOH buffer at the same pH Arabidopsis thaliana

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.2.1.76 6.5 8 pH 6.5: about 40% of maximal activity, pH 8.0: about 80% of maximal activity Arabidopsis thaliana