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Literature summary extracted from

  • Kitago, Y.; Karita, S.; Watanabe, N.; Kamiya, M.; Aizawa, T.; Sakka, K.; Tanaka, I.
    Crystal structure of Cel44A, a glycoside hydrolase family 44 endoglucanase from Clostridium thermocellum (2007), J. Biol. Chem., 282, 35703-35711.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.4 wild-type enzyme and mutant enzymes E186Q and E359Q, expression in Escherichia coli Acetivibrio thermocellus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.1.4 hanging drop vapor diffusion method at 20°C, crystal structure of Cel44A is solved at a resolution of 0.96 A.The crystal structures of E186Q mutant complexed with cellopentaose and cellohexaose are solved at 2.0 and 1.8 A resolution, respectively Acetivibrio thermocellus

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.4 E186Q mutant has no cellulase activity Acetivibrio thermocellus
3.2.1.4 E359Q mutant has no cellulase activity Acetivibrio thermocellus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.4 Acetivibrio thermocellus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.4
-
 Acetivibrio thermocellus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.4 carboxymethylcellulose + H2O
-
 Acetivibrio thermocellus ?
-
 ?
3.2.1.4 cellohexaitol + H2O
-
 Acetivibrio thermocellus ?
-
 ?
3.2.1.4 cellohexaose + H2O
-
 Acetivibrio thermocellus ?
-
 ?
3.2.1.4 cellopentaose + H2O
-
 Acetivibrio thermocellus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.4 Cel44A
-
 Acetivibrio thermocellus