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Literature summary extracted from

  • Lee, H.J.; Chen, Y.S.; Chou, C.Y.; Chien, C.H.; Lin, C.H.; Chang, G.G.; Chen, X.
    Investigation of the dimer interface and substrate specificity of prolyl dipeptidase DPP8 (2006), J. Biol. Chem., 281, 38653-38662.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.13.9 expression of His-tagged wild-type and mutant enzymes in HEK-293T cells, expression in Spodoptera frugiperda Sf9 cells via baculovirus infection system Homo sapiens
3.4.13.18 expressed in Sf9 insect cells and 293-T cells Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
3.4.13.9 F822A site-directed mutagenesis, the mutant enzyme shows reduced activity compared to the wild-type enzyme Homo sapiens
3.4.13.9 H859A site-directed mutagenesis, the mutant enzyme shows reduced activity compared to the wild-type enzyme Homo sapiens
3.4.13.9 V833A site-directed mutagenesis, the mutant enzyme shows reduced activity compared to the wild-type enzyme Homo sapiens
3.4.13.9 Y844A site-directed mutagenesis, the mutant enzyme shows reduced activity compared to the wild-type enzyme Homo sapiens
3.4.13.18 F822A decreased activity Homo sapiens
3.4.13.18 H859A decreased activity Homo sapiens
3.4.13.18 V833A decreased activity Homo sapiens
3.4.13.18 Y844A decreased activity Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.13.9 additional information
-
 additional information kinetics and substrate specificity of wild-type and mutant enzymes, overview Homo sapiens
3.4.13.9 0.127
-
 Ala-Pro pH 7.8, 37°C, recombinant His-tagged wild-type enzyme Homo sapiens
3.4.13.9 1.495
-
 Ala-Pro pH 7.8, 37°C, recombinant His-tagged mutant F822A Homo sapiens
3.4.13.9 2.219
-
 Ala-Pro pH 7.8, 37°C, recombinant His-tagged mutant V833A Homo sapiens
3.4.13.9 2.397
-
 Ala-Pro pH 7.8, 37°C, recombinant His-tagged mutant Y844A Homo sapiens
3.4.13.9 2.72
-
 Ala-Pro pH 7.8, 37°C, recombinant His-tagged mutant H859A Homo sapiens
3.4.13.18 0.016
-
 Lys-Pro-p-nitroanilide wild type enzyme Homo sapiens
3.4.13.18 0.023
-
 Leu-Pro-p-nitroanilide wild type enzyme Homo sapiens
3.4.13.18 0.027
-
 Arg-Pro-p-nitroanilide wild type enzyme Homo sapiens
3.4.13.18 0.029
-
 Met-Pro-p-nitroanilide wild type enzyme Homo sapiens
3.4.13.18 0.127
-
 Ala-Pro-p-nitroanilide wild type enzyme Homo sapiens
3.4.13.18 0.238
-
 Ser-Pro-p-nitroanilide wild type enzyme Homo sapiens
3.4.13.18 0.27
-
 Arg-Pro-p-nitroanilide mutant enzyme H859A Homo sapiens
3.4.13.18 0.325
-
 Met-Pro-p-nitroanilide mutant enzyme H859A Homo sapiens
3.4.13.18 0.348
-
 Leu-Pro-p-nitroanilide mutant enzyme H859A Homo sapiens
3.4.13.18 0.39
-
 Leu-Pro-p-nitroanilide mutant enzyme F822A Homo sapiens
3.4.13.18 0.507
-
 Lys-Pro-p-nitroanilide mutant enzyme H859A Homo sapiens
3.4.13.18 0.528
-
 Gly-Pro-p-nitroanilide wild type enzyme Homo sapiens
3.4.13.18 0.988
-
 Met-Pro-p-nitroanilide mutant enzyme F822A Homo sapiens
3.4.13.18 1.358
-
 Lys-Pro-p-nitroanilide mutant enzyme F822A Homo sapiens
3.4.13.18 1.495
-
 Ala-Pro-p-nitroanilide mutant enzyme F822A Homo sapiens
3.4.13.18 1.673
-
 Arg-Pro-p-nitroanilide mutant enzyme F822A Homo sapiens
3.4.13.18 2.219
-
 Ala-Pro-p-nitroanilide mutant enzyme V833A Homo sapiens
3.4.13.18 2.249
-
 Ser-Pro-p-nitroanilide mutant enzyme F822A Homo sapiens
3.4.13.18 2.397
-
 Ala-Pro-p-nitroanilide mutant enzyme Y844A Homo sapiens
3.4.13.18 2.668
-
 Asp-Pro-p-nitroanilide wild type enzyme Homo sapiens
3.4.13.18 2.72
-
 Ala-Pro-p-nitroanilide mutant enzyme H859A Homo sapiens
3.4.13.18 2.731
-
 Gly-Pro-p-nitroanilide mutant enzyme H859A Homo sapiens
3.4.13.18 4.599
-
 Gly-Pro-p-nitroanilide mutant enzyme F822A Homo sapiens
3.4.13.18 7.462
-
 Ser-Pro-p-nitroanilide mutant enzyme H859A Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.13.9 cytoplasm
-
 Homo sapiens 5737
-
3.4.13.18 cytoplasm
-
 Homo sapiens 5737
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.13.9 additional information
-
 hydrodynamic properties of wild-type and mutant DPP8s Homo sapiens
3.4.13.9 105000
-
 2 * 105000, recombinant His-tagged enzyme, SDS-PAGE Homo sapiens
3.4.13.9 210000
-
 analytical ultracentrifugation Homo sapiens
3.4.13.18 105000
-
 2 * 105000, His-tagged enzyme, SDS-PAGE Homo sapiens
3.4.13.18 210000
-
 His-tagged enzyme, SDS-PAGE Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.4.13.9 Homo sapiens
-
-
-
3.4.13.18 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.13.9 recombinant His-tagged wild-type and mutant enzymes from HEK-293T cells by nickel affinity and anion exchange chromatography, followed by gel filtration Homo sapiens
3.4.13.18 Ni-NTA column chromatography, Q-Sepharose column chromatography, and Superdex 200 HR gel filtration Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.13.9 Ala-Pro + H2O
-
 Homo sapiens Ala + Pro
-
 ?
3.4.13.9 Arg-Pro + H2O preferred substrate Homo sapiens Arg + Pro
-
 ?
3.4.13.9 Asp-Pro + H2O
-
 Homo sapiens Asp + Pro
-
 ?
3.4.13.9 Gly-Pro + H2O
-
 Homo sapiens Gly + Pro
-
 ?
3.4.13.9 Leu-Pro + H2O
-
 Homo sapiens Leu + Pro
-
 ?
3.4.13.9 Lys-Pro + H2O
-
 Homo sapiens Lys + Pro
-
 ?
3.4.13.9 Met-Pro + H2O high activity Homo sapiens Met + Pro
-
 ?
3.4.13.9 additional information substrate specificity of wild-type and mutant enzymes, overview Homo sapiens ?
-
 ?
3.4.13.9 Ser-Pro + H2O
-
 Homo sapiens Ser + Pro
-
 ?
3.4.13.18 Ala-Pro-p-nitroanilide + H2O
-
 Homo sapiens Ala-Pro + p-nitroaniline
-
 ?
3.4.13.18 Arg-Pro-p-nitroanilide + H2O
-
 Homo sapiens Arg-Pro + p-nitroaniline
-
 ?
3.4.13.18 Asp-Pro-p-nitroanilide + H2O
-
 Homo sapiens Asp-Pro + p-nitroaniline
-
 ?
3.4.13.18 Gly-Pro-p-nitroanilide + H2O
-
 Homo sapiens Gly-Pro + p-nitroaniline
-
 ?
3.4.13.18 Leu-Pro-p-nitroanilide + H2O
-
 Homo sapiens Leu-Pro + p-nitroaniline
-
 ?
3.4.13.18 Lys-Pro-p-nitroanilide + H2O
-
 Homo sapiens Lys-Pro + p-nitroaniline
-
 ?
3.4.13.18 Met-Pro-p-nitroanilide + H2O
-
 Homo sapiens Met-Pro + p-nitroaniline
-
 ?
3.4.13.18 Ser-Pro-p-nitroanilide + H2O
-
 Homo sapiens Ser-Pro + p-nitroaniline
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.13.9 dimer 2 * 105000, recombinant His-tagged enzyme, SDS-PAGE Homo sapiens
3.4.13.9 More hydrodynamic properties of wild-type and mutant DPP8s, overview Homo sapiens
3.4.13.18 homodimer 2 * 105000, His-tagged enzyme, SDS-PAGE Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
3.4.13.9 DPP8
-
 Homo sapiens
3.4.13.9 More DPP8 belongs to the family of prolyl dipeptidases Homo sapiens
3.4.13.9 prolyl dipeptidase
-
 Homo sapiens
3.4.13.18 DPP8
-
 Homo sapiens
3.4.13.18 prolyl dipeptidase
-
 Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.13.9 0.2
-
 Ala-Pro pH 7.8, 37°C, recombinant His-tagged mutants F822A and Y844A Homo sapiens
3.4.13.9 1
-
 Ala-Pro pH 7.8, 37°C, recombinant His-tagged mutant V833A Homo sapiens
3.4.13.9 8
-
 Ala-Pro pH 7.8, 37°C, recombinant His-tagged mutant H859A Homo sapiens
3.4.13.9 22
-
 Ala-Pro pH 7.8, 37°C, recombinant His-tagged wild-type enzyme Homo sapiens
3.4.13.18 0.1
-
 Ser-Pro-p-nitroanilide mutant enzyme F822A Homo sapiens
3.4.13.18 0.1
-
 Leu-Pro-p-nitroanilide mutant enzyme F822A Homo sapiens
3.4.13.18 0.2
-
 Gly-Pro-p-nitroanilide mutant enzyme F822A Homo sapiens
3.4.13.18 0.2
-
 Ala-Pro-p-nitroanilide mutant enzyme F822A Homo sapiens
3.4.13.18 0.2
-
 Lys-Pro-p-nitroanilide mutant enzyme F822A Homo sapiens
3.4.13.18 0.2
-
 Ala-Pro-p-nitroanilide mutant enzyme Y844A Homo sapiens
3.4.13.18 0.3
-
 Met-Pro-p-nitroanilide mutant enzyme F822A Homo sapiens
3.4.13.18 0.4
-
 Arg-Pro-p-nitroanilide mutant enzyme F822A Homo sapiens
3.4.13.18 0.9
-
 Asp-Pro-p-nitroanilide wild type enzyme Homo sapiens
3.4.13.18 1
-
 Ala-Pro-p-nitroanilide mutant enzyme V833A Homo sapiens
3.4.13.18 1.2
-
 Arg-Pro-p-nitroanilide mutant enzyme H859A Homo sapiens
3.4.13.18 1.4
-
 Gly-Pro-p-nitroanilide mutant enzyme H859A Homo sapiens
3.4.13.18 2.4
-
 Lys-Pro-p-nitroanilide mutant enzyme H859A Homo sapiens
3.4.13.18 3
-
 Met-Pro-p-nitroanilide mutant enzyme H859A Homo sapiens
3.4.13.18 3.2
-
 Lys-Pro-p-nitroanilide wild type enzyme Homo sapiens
3.4.13.18 3.8
-
 Leu-Pro-p-nitroanilide mutant enzyme H859A Homo sapiens
3.4.13.18 7
-
 Leu-Pro-p-nitroanilide wild type enzyme Homo sapiens
3.4.13.18 8
-
 Ala-Pro-p-nitroanilide mutant enzyme H859A Homo sapiens
3.4.13.18 13
-
 Ser-Pro-p-nitroanilide mutant enzyme H859A Homo sapiens
3.4.13.18 14
-
 Arg-Pro-p-nitroanilide wild type enzyme Homo sapiens
3.4.13.18 15
-
 Met-Pro-p-nitroanilide wild type enzyme Homo sapiens
3.4.13.18 19
-
 Ser-Pro-p-nitroanilide wild type enzyme Homo sapiens
3.4.13.18 22
-
 Ala-Pro-p-nitroanilide wild type enzyme Homo sapiens
3.4.13.18 25
-
 Gly-Pro-p-nitroanilide wild type enzyme Homo sapiens