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Literature summary extracted from

  • Christen, B.; Christen, M.; Paul, R.; Schmid, F.; Folcher, M.; Jenoe, P.; Meuwly, M.; Jenal, U.
    Allosteric control of cyclic di-GMP signaling (2006), J. Biol. Chem., 281, 32015-32024.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
2.7.7.65 DELTAR359/DELTAD362 mutations in allosteric binding site of cyclic diguanylate, abolish cyclic diguanylate binding, strong decrease in catalytic activity Caulobacter vibrioides
2.7.7.65 E370Q mutation in active site, complete loss of catalytic activity without effect on allosteric binding of cyclic diguanylate Caulobacter vibrioides
2.7.7.65 EE370GG mutation in active site, complete loss of catalytic activity without effect on allosteric binding of cyclic diguanylate Caulobacter vibrioides
2.7.7.65 R148A increase in allosteric binding of cyclic diguanylate and increase in catalytic activity Caulobacter vibrioides
2.7.7.65 R148A/R178A increase in allosteric binding of cyclic diguanylate and increase in catalytic activity Caulobacter vibrioides
2.7.7.65 R178A increase in allosteric binding of cyclic diguanylate and increase in catalytic activity Caulobacter vibrioides
2.7.7.65 R359A mutation in allosteric binding site of cyclic diguanylate, strong decrease in cyclic diguanylate binding, strong decrease in catalytic activity Caulobacter vibrioides
2.7.7.65 R359V strong decrease in catalytic activity Caulobacter vibrioides
2.7.7.65 R390A mutation in allosteric binding site of cyclic diguanylate, strong decrease in cyclic diguanylate binding. Catalytic activity comaprable to wild-type Caulobacter vibrioides

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.65 cyclic di-3',5'-guanylate noncompetitive product inhibition at an allosteric binding site fully contained within the GGDEF domain. Core of the binding site is an RXXD motif formed by residues R359, D362, and R390 Caulobacter vibrioides

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.65 Caulobacter vibrioides Q9A3B9
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.65 GTP
-
 Caulobacter vibrioides cyclic di-3',5'-guanylate + diphosphate
-
 ?

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.7.65 0.0028
-
 cyclic di-3',5'-guanylate mutant R148A, 30°C, pH 8.0 Caulobacter vibrioides
2.7.7.65 0.0029
-
 cyclic di-3',5'-guanylate mutant R148A/R178A, 30°C, pH 8.0 Caulobacter vibrioides
2.7.7.65 0.0036
-
 cyclic di-3',5'-guanylate mutant R178A, 30°C, pH 8.0 Caulobacter vibrioides
2.7.7.65 0.0058
-
 cyclic di-3',5'-guanylate wild-type, 30°C, pH 8.0 Caulobacter vibrioides
2.7.7.65 0.1
-
 cyclic di-3',5'-guanylate or above, mutant R359A, 30°C, pH 8.0 Caulobacter vibrioides
2.7.7.65 0.115
-
 cyclic di-3',5'-guanylate mutant R390A, 30°C, pH 8.0 Caulobacter vibrioides