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Literature summary extracted from

  • Martinez-Fleites, C.; Guerreiro, C.I.; Baumann, M.J.; Taylor, E.J.; Prates, J.A.; Ferreira, L.M.; Fontes, C.M.; Brumer, H.; Davies, G.J.
    Crystal structures of Clostridium thermocellum xyloglucanase, XGH74A, reveal the structural basis for xyloglucan recognition and degradation (2006), J. Biol. Chem., 281, 24922-24933.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.151 expression in Escherichia coli Acetivibrio thermocellus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.1.151 unliganded wild-type and mutant D70A in complex with oligosaccharide substrate Acetivibrio thermocellus

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.151 D74A crystallization data in complex with oligosaccharide substrate Acetivibrio thermocellus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.151 additional information
-
 additional information Km values are 0.76 mg/ml for xyloglucan, 4.6 mg/ml for carboxymethyl cellulose, 6.6 mg/ml for lichenan, 5.1 mg/ml for carboxyethyl cellulose Acetivibrio thermocellus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.151 Acetivibrio thermocellus
-
 isoform XGH74A
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.151 carboxyethyl cellulose + H2O
-
 Acetivibrio thermocellus carboxyethyl cellulose oligosaccharides
-
 ?
3.2.1.151 carboxymethyl cellulose + H2O carboxymethyl cellulose-4M Acetivibrio thermocellus carboxymethyl cellulose oligosaccharides
-
 ?
3.2.1.151 lichenan + H2O
-
 Acetivibrio thermocellus lichenan cellulose oligosaccharides
-
 ?
3.2.1.151 additional information enzyme does not cleave at substituted glucosyl moieties Acetivibrio thermocellus ?
-
 ?
3.2.1.151 xyloglucan + H2O source of substrate tamarind seed Acetivibrio thermocellus xyloglucan oligosaccharides
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.151 XGH74
-
 Acetivibrio thermocellus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.151 0.22
-
 carboxyethyl cellulose pH 7.0, 50°C Acetivibrio thermocellus
3.2.1.151 0.59
-
 lichenan pH 7.0, 50°C Acetivibrio thermocellus
3.2.1.151 0.72
-
 carboxymethyl cellulose pH 7.0, 50°C Acetivibrio thermocellus
3.2.1.151 0.78
-
 xyloglucan pH 7.0, 50°C Acetivibrio thermocellus