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Literature summary extracted from

  • Sandoval, F.J.; Roje, S.
    An FMN hydrolase is fused to a riboflavin kinase homolog in plants (2005), J. Biol. Chem., 280, 38337-38345.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.3.102 Tween 20 enzyme activity increases and temperature optimum shifts to 5–10°C higher temperature in the presence of Tween 20 Arabidopsis thaliana

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.26 the functional overexpression of the individual domains in Escherichia coli establishes that the riboflavin kinase and FMN hydrolase activities reside, respectively, in the C-terminal (AtFMN) and N-terminal (AtFHy) domains of AtFMN/FHy Arabidopsis thaliana
3.1.3.102 functional overexpression of the individual domains in Escherichia coli establishes that the riboflavin kinase and FMN hydrolase activities reside, respectively, in the C-terminal (AtFMN) and N-terminal (AtFHy) domains of the bifunctional riboflavin kinase/FMN phosphatase (AtFMN/FHy). Riboflavin kinase and FMN hydrolase domains of AtFMN/FHy can be physically separated, with little change in their kinetic properties Arabidopsis thaliana

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.102 EDTA the FMN hydrolase activity of the bifunctional riboflavin kinase/FMN phosphatase greatly decreases when EDTA replaced Mg2+ in the assays Arabidopsis thaliana
3.1.3.102 phosphate the FMN hydrolase activity of the bifunctional riboflavin kinase/FMN phosphatase is inhibited Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.26 0.0000103
-
 riboflavin bifunctional enzyme AtFMN/FHy Arabidopsis thaliana
2.7.1.26 0.0002
-
 ATP bifunctional enzyme AtFMN/FHy Arabidopsis thaliana
3.1.3.102 0.0008
-
 FMN pH 7.5, 30°C Arabidopsis thaliana

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.102 Mg2+ the FMN hydrolase activity of the bifunctional riboflavin kinase/FMN phosphatase greatly decreases when EDTA replaced Mg2+ in the assays Arabidopsis thaliana

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.102 25700
-
 FMN hydrolase domains of the bifunctional riboflavin kinase/FMN phosphatase, SDS-PAGE Arabidopsis thaliana
3.1.3.102 42100
-
-
 Arabidopsis thaliana
3.1.3.102 43600
-
 bifunctional riboflavin kinase/FMN phosphatase, gel filtration Arabidopsis thaliana

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.26 Arabidopsis thaliana
-
 ecotype Columbia
-
3.1.3.102 Arabidopsis thaliana Q84MD8 bifunctional riboflavin kinase/FMN phosphatase
-
3.1.3.102 Arabidopsis thaliana ecotype Columbia Q84MD8 bifunctional riboflavin kinase/FMN phosphatase
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.102
-
 Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.26 ATP + riboflavin
-
 Arabidopsis thaliana ADP + FMN
-
 ?
3.1.3.102 FMN + H2O
-
 Arabidopsis thaliana riboflavin + phosphate
-
 ?
3.1.3.102 FMN + H2O
-
 Arabidopsis thaliana ecotype Columbia riboflavin + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.102 monomer 1 * 42100, bifunctional riboflavin kinase/FMN phosphatase, SDS-PAGE Arabidopsis thaliana

Synonyms

EC Number Synonyms Comment Organism
2.7.1.26 AtFMN/FHy bifunctional enzyme has riboflavin kinase and FMN hydrolase activities Arabidopsis thaliana
3.1.3.102 At4g21470 gene name, bifunctional riboflavin kinase/FMN phosphatase Arabidopsis thaliana
3.1.3.102 AtFMN/FHy bifunctional riboflavin kinase/FMN phosphatase Arabidopsis thaliana

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.102 30
-
 assay at Arabidopsis thaliana

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.3.102 0.822
-
 FMN pH 7.5, 30°C Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.102 5.5
-
-
 Arabidopsis thaliana

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.3.102 5 8 pH 5.0: about 90% of maximal activity, pH 8.0: about 60% of maximal activity Arabidopsis thaliana

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.1.3.102 1028
-
 FMN pH 7.5, 30°C Arabidopsis thaliana