EC Number | Application | Comment | Organism |
---|---|---|---|
3.1.21.6 | additional information | deoxyribonuclease is an endonuclease, but is not a restriction endonuclease | Mycolicibacterium smegmatis |
EC Number | General Stability | Organism |
---|---|---|
3.1.30.2 | addition of 10 mM EDTA essential for storage stability | Mycolicibacterium smegmatis |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.1.21.6 | EDTA | chelates Mg2+, increasing Mg2+ concentrations can restore the activity of the enzyme | Mycolicibacterium smegmatis | |
3.1.21.6 | K+ | - |
Mycolicibacterium smegmatis | |
3.1.21.6 | additional information | not inhibited by Na+ | Mycolicibacterium smegmatis | |
3.1.30.2 | EDTA | 90% inhibition at 2.5 mM, inhibition fully reversible by addition of Mg2+ | Mycolicibacterium smegmatis | |
3.1.30.2 | K+ | - |
Mycolicibacterium smegmatis | |
3.1.30.2 | additional information | no inhibition by Na+ | Mycolicibacterium smegmatis |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.1.21.6 | Mg2+ | required for activity, maximum activity at 2.5 mM | Mycolicibacterium smegmatis | |
3.1.21.6 | Mn2+ | can replace Mg2+ showing better activity | Mycolicibacterium smegmatis | |
3.1.30.2 | Mg2+ | optimum activity in the presence of 2.5 mM | Mycolicibacterium smegmatis | |
3.1.30.2 | Mn2+ | can replace Mg2+, more effective than Mg2+ | Mycolicibacterium smegmatis |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.1.21.6 | 43000 | - |
gel filtration | Mycolicibacterium smegmatis |
3.1.30.2 | 43000 | - |
x * 43000, SDS-PAGE | Mycolicibacterium smegmatis |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.30.2 | dsDNA + H2O | Mycolicibacterium smegmatis | - |
? | DNA fragments carrying phosphate groups at 5' ends and hydroxyl group at the 3' ends | ? | |
3.1.30.2 | dsDNA + H2O | Mycolicibacterium smegmatis | physiological function currently unknown | ? | DNA fragments carrying phosphate groups at 5' ends and hydroxyl group at the 3' ends, random DNA cleavage with respect to all possible phosphodiester bonds | ? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.21.6 | Mycolicibacterium smegmatis | - |
mc26 culture | - |
3.1.30.2 | Mycolicibacterium smegmatis | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.21.6 | to near homogeneity, 521fold by gel filtration | Mycolicibacterium smegmatis |
3.1.30.2 | purified to near homogeneity | Mycolicibacterium smegmatis |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.1.21.6 | 18 | - |
crude extract | Mycolicibacterium smegmatis |
3.1.21.6 | 9444 | - |
521fold purified enzyme | Mycolicibacterium smegmatis |
3.1.30.2 | 0.315 | - |
purified enzyme | Mycolicibacterium smegmatis |
EC Number | Storage Stability | Organism |
---|---|---|
3.1.21.6 | 0°C, Tris-HCl buffer, pH 7.4, 10 mM EDTA, 10% glycerol, stable for at least 15 days | Mycolicibacterium smegmatis |
3.1.30.2 | 0°C, Tris-HCl buffer, pH 7.4, 10 mM EDTA, 10% glycerol, 15 days storage on ice, fully stable for at least 15 days | Mycolicibacterium smegmatis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.21.6 | covalently closed circular DNA pUC18 + H2O | - |
Mycolicibacterium smegmatis | ? | - |
? | |
3.1.21.6 | lambda DNA + H2O | - |
Mycolicibacterium smegmatis | ? | - |
? | |
3.1.21.6 | additional information | catalyzes the endonucleolytic cleavage of both the double- as well as the single-strand DNA non-specifically | Mycolicibacterium smegmatis | ? | - |
? | |
3.1.21.6 | pBR322 DNA + H2O | - |
Mycolicibacterium smegmatis | DNA fragments carrying phosphate groups at 5' ends and hydroxyl group at the 3' ends, respectively | - |
? | |
3.1.30.2 | DNA + H2O | tritium labeled DNA from Escherichia coli, fragmented by limited sonication | Mycolicibacterium smegmatis | ? | random DNA cleavage with respect to all possible phosphodiester bonds | ? | |
3.1.30.2 | dsDNA + H2O | - |
Mycolicibacterium smegmatis | ? | DNA fragments carrying phosphate groups at 5' ends and hydroxyl group at the 3' ends | ? | |
3.1.30.2 | dsDNA + H2O | physiological function currently unknown | Mycolicibacterium smegmatis | ? | DNA fragments carrying phosphate groups at 5' ends and hydroxyl group at the 3' ends, random DNA cleavage with respect to all possible phosphodiester bonds | ? | |
3.1.30.2 | lambda DNA + H2O | - |
Mycolicibacterium smegmatis | ? | size of linear DNA fragments decreases with prolonged incubation time | ? | |
3.1.30.2 | pUC18 DNA + H2O | covalently closed circular plasmid DNA | Mycolicibacterium smegmatis | ? | substrate converted into relaxed circular form and than to the linear form | ? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.1.30.2 | ? | x * 43000, SDS-PAGE | Mycolicibacterium smegmatis |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.21.6 | Deoxyriboendonuclease | - |
Mycolicibacterium smegmatis |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.21.6 | 32 | - |
- |
Mycolicibacterium smegmatis |
3.1.30.2 | 32 | - |
- |
Mycolicibacterium smegmatis |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.1.21.6 | 7.2 | - |
- |
Mycolicibacterium smegmatis |
3.1.30.2 | 7.2 | - |
- |
Mycolicibacterium smegmatis |