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Literature summary extracted from

  • Lee, S.; Oneda, H.; Minoda, M.; Tanaka, A.; Inouye, K.
    Comparison of starch hydrolysis activity and thermal stability of two Bacillus licheniformis alpha-amylases and insights into engineering alpha-amylase variants active under acidic conditions (2006), J. Biochem., 139, 997-1005.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.2.1.1 stabilization by Ca2+, alpha-amylase PA Bacillus licheniformis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.1 additional information
-
 additional information KM for soluble starch is constant at pH 5.0-7.0 and increases greatly with decreasing pH from 5.0 to 2.8 and slightly with increasing pH from 7.5 to 9.2, alpha-amylase PA Bacillus licheniformis

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.1 Bacillus licheniformis
-
 IFO12196
-
3.2.1.1 Bacillus licheniformis IFO12196
-
 IFO12196
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.1 soluble starch + H2O alpha-amylase PA Bacillus licheniformis ?
-
 ?
3.2.1.1 soluble starch + H2O alpha-amylase PA Bacillus licheniformis IFO12196 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.1 alpha-amylase PA
-
 Bacillus licheniformis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.1 41
-
 T50-value at pH 4.5, without CaCl2, alpha-amylase PA Bacillus licheniformis
3.2.1.1 63
-
 T50-value at pH 4.5, 5 mM CaCl2, alpha-amylase PA Bacillus licheniformis
3.2.1.1 71
-
 T50-value at pH 6.0, without CaCl2, alpha-amylase PA Bacillus licheniformis
3.2.1.1 84
-
 T50-value at pH 6.0, 5 mM CaCl2, alpha-amylase PA Bacillus licheniformis
3.2.1.1 90
-
 pH 5.5, 1 h, 50% inactivation, alpha-amylase PA Bacillus licheniformis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.1 6
-
 starch hydrolysis at 95°C, alpha-amylase PA Bacillus licheniformis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.1 5.1 8.2 pH-range where 50% or more of the maximal activity is maintained, alpha-amylase PA Bacillus licheniformis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.1 5
-
 90°C, 1 h, complete inactivation, alpha-amylase PA Bacillus licheniformis
3.2.1.1 5.5
-
 90°C, 1 h, 50% loss of activity, alpha-amylase PA Bacillus licheniformis