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Literature summary extracted from

  • Liu, Z.; Zhen, Z.; Zuo, Z.; Wu, Y.; Liu, A.; Yi, Q.; Li, W.
    Probing the catalytic center of porcine aminoacylase 1 by site-directed mutagenesis, homology modeling and substrate docking (2006), J. Biochem., 139, 421-430.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.14 expression in E. coli Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.14 0.84
-
 N-alpha-acetyl-L-methionine native porcine enzyme,25°C Sus scrofa
3.5.1.14 1.2
-
 N-alpha-acetyl-L-methionine E309H-mutant, 25°C Sus scrofa
3.5.1.14 1.25
-
 N-alpha-acetyl-L-methionine Wild type Rosetta (DE3),25°C Sus scrofa
3.5.1.14 2.12
-
 N-alpha-acetyl-L-methionine R274A-mutant, 25°C Sus scrofa
3.5.1.14 2.21
-
 N-alpha-acetyl-L-methionine H79A-mutant, 25°C Sus scrofa
3.5.1.14 2.23
-
 N-alpha-acetyl-L-methionine E147A-mutant, 25°C Sus scrofa
3.5.1.14 2.36
-
 N-alpha-acetyl-L-methionine E174A-mutant, 25°C Sus scrofa
3.5.1.14 2.45
-
 N-alpha-acetyl-L-methionine H371A-mutant, 25°C Sus scrofa
3.5.1.14 2.52
-
 N-alpha-acetyl-L-methionine D112A-mutant, 25°C Sus scrofa
3.5.1.14 3.82
-
 N-alpha-acetyl-L-methionine H205L-mutant, 25°C Sus scrofa
3.5.1.14 4.34
-
 N-alpha-acetyl-L-methionine E146A-mutant, 25°C Sus scrofa
3.5.1.14 4.57
-
 N-alpha-acetyl-L-methionine N261A-mutant, 25°C Sus scrofa

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.14 Sus scrofa P37111
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.1.14 kidney
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.14 6.4
-
 H79A-mutant, 25°C Sus scrofa
3.5.1.14 9.2
-
 N261A-mutant, 25°C Sus scrofa
3.5.1.14 9.4
-
 D112A-mutant, 25°C Sus scrofa
3.5.1.14 10.3
-
 Wild type BL21 (DE3), 25°C Sus scrofa
3.5.1.14 13.6
-
 E146A-mutant, 25°C Sus scrofa
3.5.1.14 25.7
-
 E147A-mutant, 25°C Sus scrofa
3.5.1.14 32.6
-
 H371A-mutant, 25°C Sus scrofa
3.5.1.14 53.6
-
 E174A-mutant, 25°C Sus scrofa
3.5.1.14 78.5
-
 H205L-mutant, 25°C Sus scrofa
3.5.1.14 197
-
 R274A-mutant, 25°C Sus scrofa
3.5.1.14 203
-
 E309H-mutant, 25°C Sus scrofa
3.5.1.14 220
-
 Wild type Rosetta (DE3), 25°C Sus scrofa
3.5.1.14 287
-
 native porcine enzyme, 25°C Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.14 N-acetyl-L-methionine + H2O
-
 Sus scrofa acetate + L-methionine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.1.14 pAcy1
-
 Sus scrofa