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Literature summary extracted from

  • Ozawa, Y.; Nakamura, T.; Kamata, N.; Yasujima, D.; Urushiyama, A.; Yamakura, F.; Ohmori, D.; Imai, T.
    Thermococcus profundus 2-ketoisovalerate ferredoxin oxidoreductase, a key enzyme in the archaeal energy-producing amino acid metabolic pathway (2005), J. Biochem., 137, 101-107.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.7.7 0.021
-
coenzyme A
-
Thermococcus profundus
1.2.7.7 0.17
-
2-ketoisovalerate
-
Thermococcus profundus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.2.7.7 4Fe-4S cluster
-
Thermococcus profundus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.7.7 13000
-
delta subunit, determined by SDS-PAGE Thermococcus profundus
1.2.7.7 22000
-
gamma subunit, determined by SDS-PAGE Thermococcus profundus
1.2.7.7 31000
-
beta subunit, determined by SDS-PAGE Thermococcus profundus
1.2.7.7 45000
-
alpha subunit, determined by SDS-PAGE Thermococcus profundus
1.2.7.7 218000
-
native enzyme, determined by gel filtration Thermococcus profundus
1.2.7.7 220000
-
native enzyme, determined by analytical centrifugation Thermococcus profundus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.7.7 3-methyl-2-oxobutanoate + CoA + oxidized ferredoxin Thermococcus profundus
-
S-(2-methylpropanoyl)-CoA + CO2 + reduced ferredoxin
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.2.7.7 Thermococcus profundus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.7.7 cell-free extracts are loaded onto a Q Sepharose Fast Flow column, activity containing fractions are loaded onto a hydroxyapatite column, finally a TOYOPEARL column is used Thermococcus profundus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.7.7 0.76
-
cell-free extract Thermococcus profundus
1.2.7.7 3.8
-
Hydroxyapatite purified Thermococcus profundus
1.2.7.7 4.3
-
Q Sepharose purified Thermococcus profundus
1.2.7.7 128
-
Red TOYOPEARL purified Thermococcus profundus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.7.7 2-ketoisovalerate + CoA + oxidized ferredoxin
-
Thermococcus profundus ?
-
?
1.2.7.7 3-methyl-2-oxobutanoate + CoA + oxidized ferredoxin
-
Thermococcus profundus S-(2-methylpropanoyl)-CoA + CO2 + reduced ferredoxin
-
?

Subunits

EC Number Subunits Comment Organism
1.2.7.7 octamer heterooctamer consisting of four different subunits Thermococcus profundus

Synonyms

EC Number Synonyms Comment Organism
1.2.7.7 2-ketoisovalerate ferredoxin oxidoreductase
-
Thermococcus profundus
1.2.7.7 3-methyl-2-oxobutanoate dehydrogenase
-
Thermococcus profundus
1.2.7.7 VOR
-
Thermococcus profundus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.2.7.7 80
-
activity assay, VOR activity is routinely assayed by means of CoA-dependent reduction of methyl viologen with 2-ketoisovalerate at 80°C Thermococcus profundus
1.2.7.7 93
-
the optimal temperature for the 2-ketoisovalerate methyl viologen oxidoreductase assay is determined to be 93°C Thermococcus profundus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.2.7.7 80 112
-
Thermococcus profundus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.7.7 8.4
-
activity assay Thermococcus profundus

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.7.7 CoA
-
Thermococcus profundus
1.2.7.7 Ferredoxin
-
Thermococcus profundus