Literature summary extracted from
Yamano, S.; Maruyama, T.
An azide-insensitive superoxide dismutase from a hyperthermophilic archaeon, Sulfolobus solfataricus (1999), J. Biochem., 125, 186-193.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
1.15.1.1 |
DNA and amino acid sequence determination and anaylsis, expression of wild-type and mutant in Escherichia coli |
Saccharolobus solfataricus |
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
1.15.1.1 |
Y88F |
site-directed mutagenesis, substitution of Tyr88 to Phe does not affect the metal specificity of the enzyme |
Saccharolobus solfataricus |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
1.15.1.1 |
fluoride |
recombinant Fe-reconstituted SOD |
Saccharolobus solfataricus |
|
1.15.1.1 |
H2O2 |
distinguishes Fe-SOD from Mn-SOD, since it inactivates only Fe-SOD |
Saccharolobus solfataricus |
|
1.15.1.1 |
additional information |
recombinant Fe-reconstituted SOD is not inhibited by azide, steric hindrance in the substrate funnel of the enzyme prevents the access of N3- but allows O2- and F- access to the active site |
Saccharolobus solfataricus |
|
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
1.15.1.1 |
Fe2+ |
Fe/Mn-type SOD |
Saccharolobus solfataricus |
|
1.15.1.1 |
Mn2+ |
Fe/Mn-type SOD |
Saccharolobus solfataricus |
|
1.15.1.1 |
additional information |
metal content analysis of the recombinant enzyme |
Saccharolobus solfataricus |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
1.15.1.1 |
Saccharolobus solfataricus |
P80857 |
DSM 1616 |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
1.15.1.1 |
recombinant wild-type and mutant from Escherichia coli |
Saccharolobus solfataricus |
Renatured (Commentary)
EC Number |
Renatured (Comment) |
Organism |
---|
1.15.1.1 |
reconstitution of Fe-SOD from purified recombinant apo-enzyme, apo-enzyme preparation: purified recombinant enzyme is denatured in buffer containing 50 mM acetate buffer, pH 3.8, 6 M guanidine hydrochloride, and 10 mM EDTA for 16 h at 50°C, followed by dialysis against the same buffer containing MnSO4 instead of EDTA, and removal of guanidine hydrochloride in a second dialysis step, followed by gel filtration, overview |
Saccharolobus solfataricus |
Source Tissue
EC Number |
Source Tissue |
Comment |
Organism |
Textmining |
---|
1.15.1.1 |
additional information |
Sulfolobus solfataricus grows between 50°C and 87°C with an optimum growing temperature of 87°C, but it neither grows nor survives at 90°C |
Saccharolobus solfataricus |
- |
Specific Activity [micromol/min/mg]
EC Number |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Comment |
Organism |
---|
1.15.1.1 |
1.2 |
- |
reconstituted Mn-SOD |
Saccharolobus solfataricus |
1.15.1.1 |
4.3 |
- |
reconstituted Mn-SOD mutant Y88F |
Saccharolobus solfataricus |
1.15.1.1 |
481 |
- |
reconstituted Fe-SOD |
Saccharolobus solfataricus |
1.15.1.1 |
755 |
- |
reconstituted Fe-SOD mutant Y88F |
Saccharolobus solfataricus |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
1.15.1.1 |
SOD |
- |
Saccharolobus solfataricus |
Temperature Optimum [°C]
EC Number |
Temperature Optimum [°C] |
Temperature Optimum Maximum [°C] |
Comment |
Organism |
---|
1.15.1.1 |
25 |
- |
assay at |
Saccharolobus solfataricus |
Temperature Stability [°C]
EC Number |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Comment |
Organism |
---|
1.15.1.1 |
95 |
- |
2 h, purified recombinant enzyme, 96% remaining activity, half-life is 33 h, inactivation according to first order kinetics |
Saccharolobus solfataricus |
1.15.1.1 |
100 |
- |
purified recombinant enzyme, half-life is 8.7 h, inactivation according to first order kinetics |
Saccharolobus solfataricus |
1.15.1.1 |
105 |
- |
purified recombinant enzyme, half-life is 1.5 h, inactivation according to first order kinetics |
Saccharolobus solfataricus |
pH Optimum
EC Number |
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
---|
1.15.1.1 |
7.8 |
- |
assay at |
Saccharolobus solfataricus |