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Literature summary extracted from

  • Lee, H.S.; Cho, Y.; Lee, J.H.; Kang, S.G.
    Novel monofunctional histidinol-phosphate phosphatase of the DDDD superfamily of phosphohydrolases (2008), J. Bacteriol., 190, 2629-2632.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.15 0.03
-
 L-histidinol phosphate in the presence of Mn2+, at 80°C and pH 6.5 Thermococcus onnurineus
3.1.3.15 0.05
-
 L-histidinol phosphate in the presence of Co2+, at 80°C and pH 6.5 Thermococcus onnurineus
3.1.3.15 0.09
-
 L-histidinol phosphate in the presence of Mg2+, at 80°C and pH 6.5 Thermococcus onnurineus
3.1.3.15 0.09
-
 L-histidinol phosphate in the presence of Ni2+, at 80°C and pH 6.5 Thermococcus onnurineus
3.1.3.15 0.59
-
 AMP in the presence of 1 mM Co2+, at 80°C and pH 6.5 Thermococcus onnurineus
3.1.3.15 1.45
-
 L-phosphoserine in the presence of 1 mM Co2+, at 80°C and pH 6.5 Thermococcus onnurineus
3.1.3.15 2.37
-
 D-fructose 6-phosphate in the presence of 1 mM Co2+, at 80°C and pH 6.5 Thermococcus onnurineus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.15 Co2+ strong activity in the presence of Co2+ Thermococcus onnurineus
3.1.3.15 Cu2+ strong activity in the presence of Cu2+ Thermococcus onnurineus
3.1.3.15 Mg2+ strong activity in the presence of Mg2+ Thermococcus onnurineus
3.1.3.15 Mn2+ strong activity in the presence of Mn2+ Thermococcus onnurineus
3.1.3.15 Ni2+ strong activity in the presence of Ni2+ Thermococcus onnurineus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.15 39000
-
 gel filtration Thermococcus onnurineus

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.15 Thermococcus onnurineus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.15 TALON metal affinity column chromatography and Superdex 200 10/300 gel filtration Thermococcus onnurineus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.15 AMP + H2O
-
 Thermococcus onnurineus ?
-
 ?
3.1.3.15 D-fructose 6-phosphate + H2O
-
 Thermococcus onnurineus ?
-
 ?
3.1.3.15 L-histidinol phosphate + H2O
-
 Thermococcus onnurineus L-histidinol + phosphate
-
 ?
3.1.3.15 L-phosphoserine + H2O
-
 Thermococcus onnurineus L-serine + phosphate
-
 ?
3.1.3.15 additional information does not show any phosphatase activity with p-nitrophenyl phosphate Thermococcus onnurineus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.15 monomer 1 * 39000, gel filtration Thermococcus onnurineus

Synonyms

EC Number Synonyms Comment Organism
3.1.3.15 histidinol phosphate phosphatase
-
 Thermococcus onnurineus
3.1.3.15 HolPase member of the DDDD phosphohydrolase/phosphotransferase superfamily Thermococcus onnurineus
3.1.3.15 TON_0887-derived protein
-
 Thermococcus onnurineus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.3.15 11
-
 D-fructose 6-phosphate in the presence of 1 mM Co2+, at 80°C and pH 6.5 Thermococcus onnurineus
3.1.3.15 14
-
 L-phosphoserine in the presence of 1 mM Co2+, at 80°C and pH 6.5 Thermococcus onnurineus
3.1.3.15 29
-
 AMP in the presence of 1 mM Co2+, at 80°C and pH 6.5 Thermococcus onnurineus
3.1.3.15 260
-
 L-histidinol phosphate in the presence of 1 mM Co2+, at 80°C and pH 6.5 Thermococcus onnurineus
3.1.3.15 290
-
 L-histidinol phosphate in the presence of 1 mM Ni2+, at 80°C and pH 6.5 Thermococcus onnurineus
3.1.3.15 320
-
 L-histidinol phosphate in the presence of 1 mM Mn2+, at 80°C and pH 6.5 Thermococcus onnurineus
3.1.3.15 420
-
 L-histidinol phosphate in the presence of 1 mM Mg2+, at 80°C and pH 6.5 Thermococcus onnurineus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.15 6.5
-
-
 Thermococcus onnurineus