BRENDA - Enzyme Database

Role of phosphoenolpyruvate in the NADP-isocitrate dehydrogenase and isocitrate lyase reaction in Escherichia coli

Ogawa, T.; Murakami, K.; Mori, H.; Ishii, N.; Tomita, M.; Yoshin, M.; J. Bacteriol. 189, 1176-1178 (2007)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.42
phosphoenolpyruvate
allosteric inhibition. Phosphoenolpyruvate enhances the uncompetitive inhibition of isocitrate lyase by increasing isocitrate, which protects isocitrate dehydrogenase from the inhibition, and contributes to the control through the tricarboxylic acid cycle and glyoxylate shunt
Escherichia coli
4.1.3.1
phosphoenolpyruvate
uncompetitive inhibitor
Escherichia coli
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.42
0.029
-
isocitrate
-
Escherichia coli
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.1.42
Escherichia coli
-
-
-
4.1.3.1
Escherichia coli
-
-
-
4.1.3.1
Escherichia coli K-12 W3110
-
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.42
isocitrate + NADP+
-
680481
Escherichia coli
2-oxoglutarate + CO2 + NADPH
-
-
-
?
1.1.1.42
isocitrate + NADP+
-
680481
Escherichia coli K-12 W3110
2-oxoglutarate + CO2 + NADPH
-
-
-
?
4.1.3.1
isocitrate
-
680481
Escherichia coli
succinate + glyoxylate
-
-
-
?
4.1.3.1
isocitrate
-
680481
Escherichia coli K-12 W3110
succinate + glyoxylate
-
-
-
?
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.1.1.42
0.31
-
phosphoenolpyruvate
pH 7.1
Escherichia coli
4.1.3.1
0.893
-
phosphoenolpyruvate
in 100 mM potassium phosphate buffer (pH 7.1), 10 mM MgCl2, 12 mM cysteine
Escherichia coli
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.42
phosphoenolpyruvate
allosteric inhibition. Phosphoenolpyruvate enhances the uncompetitive inhibition of isocitrate lyase by increasing isocitrate, which protects isocitrate dehydrogenase from the inhibition, and contributes to the control through the tricarboxylic acid cycle and glyoxylate shunt
Escherichia coli
4.1.3.1
phosphoenolpyruvate
uncompetitive inhibitor
Escherichia coli
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.1.1.42
0.31
-
phosphoenolpyruvate
pH 7.1
Escherichia coli
4.1.3.1
0.893
-
phosphoenolpyruvate
in 100 mM potassium phosphate buffer (pH 7.1), 10 mM MgCl2, 12 mM cysteine
Escherichia coli
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.42
0.029
-
isocitrate
-
Escherichia coli
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.42
isocitrate + NADP+
-
680481
Escherichia coli
2-oxoglutarate + CO2 + NADPH
-
-
-
?
1.1.1.42
isocitrate + NADP+
-
680481
Escherichia coli K-12 W3110
2-oxoglutarate + CO2 + NADPH
-
-
-
?
4.1.3.1
isocitrate
-
680481
Escherichia coli
succinate + glyoxylate
-
-
-
?
4.1.3.1
isocitrate
-
680481
Escherichia coli K-12 W3110
succinate + glyoxylate
-
-
-
?