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Literature summary extracted from

  • Panbangred, W.; Weeradechapon, K.; Udomvaraphant, S.; Fujiyama, K.; Meevootisom, V.
    High expression of the penicillin G acylase gene (pac) from Bacillus megaterium UN1 in its own pac minus mutant (2000), J. Appl. Microbiol., 89, 152-157.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.5.1.11 synthesis production of 6-aminopenicillanate as precursor for the production of semi-synthetic beta-lactam derivatives Priestia megaterium

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.11 expressed in enzyme deficient Bacillus megaterium strain UN-cat, 20fold higher expression rate than in host strain Priestia megaterium

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.5.1.11 extracellular
-
 Priestia megaterium
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.11 27000
-
 1 * 27000 + 1 * 57000, alpha, beta, SDS-PAGE, the alpha and beta subunits range from residue 25 to 265 and from 266 to 802 Priestia megaterium
3.5.1.11 57000
-
 1 * 27000 + 1 * 57000, alpha, beta, SDS-PAGE, the alpha and beta subunits range from residue 25 to 265 and from 266 to 802 Priestia megaterium

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.11 penicillin G + H2O Priestia megaterium
-
 6-aminopenicillanic acid + phenylacetic acid
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.11 Priestia megaterium
-
 strain UN1 and enzyme deficient strain UN-cat
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.11 recombinant enzyme from Bacillus megaterium UN-cat Priestia megaterium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.11 penicillin G + H2O
-
 Priestia megaterium 6-aminopenicillanic acid + phenylacetic acid
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.11 dimer 1 * 27000 + 1 * 57000, alpha, beta, SDS-PAGE, the alpha and beta subunits range from residue 25 to 265 and from 266 to 802 Priestia megaterium

Synonyms

EC Number Synonyms Comment Organism
3.5.1.11 PAC
-
 Priestia megaterium
3.5.1.11 penicillin G acylase
-
 Priestia megaterium