BRENDA - Enzyme Database

Inhibition of the pathogenicity of Magnaporthe grisea by bromophenols, isocitrate lyase inhibitors, from the red alga Odonthalia corymbifera

Lee, H.S.; Lee, T.H.; Lee, J.H.; Chae, C.S.; Chung, S.C.; Shin, D.S.; Shin, J.; Oh, K.B.; J. Agric. Food Chem. 55, 6923-6928 (2007)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
4.1.3.1
expressed in Escherichia coli strain BLR (DE3)
Magnaporthe grisea
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
4.1.3.1
2,2',3,3'-tetrabromo-4,4',5,5'-tetrahydroxydiphenylmethane
-
Magnaporthe grisea
4.1.3.1
2,2',3-tribromo-3',4,4',5-tetrahydroxy-6'-hydroxymethyldiphenylmethane
-
Magnaporthe grisea
4.1.3.1
2,3-dibromo-4,5-dihydroxybenzyl alcohol
-
Magnaporthe grisea
4.1.3.1
2,3-dibromo-4,5-dihydroxybenzyl methyl ether
-
Magnaporthe grisea
4.1.3.1
3,5-dibromo-4-hydroxyphenylethylamine
-
Magnaporthe grisea
4.1.3.1
3-bromo-4-(2,3-dibromo-4,5-dihydroxybenzyl)-5-methoxymethylpyrocatechol
-
Magnaporthe grisea
4.1.3.1
3-nitropropionate
-
Magnaporthe grisea
4.1.3.1
additional information
not inhibited by bromopyruvate
Magnaporthe grisea
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.1.3.1
Magnaporthe grisea
-
-
-
4.1.3.1
Magnaporthe grisea Guy 11
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
4.1.3.1
Ni-NTA column chromatography
Magnaporthe grisea
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.3.1
isocitrate
-
680310
Magnaporthe grisea
succinate + glyoxylate
-
-
-
?
4.1.3.1
isocitrate
-
680310
Magnaporthe grisea Guy 11
succinate + glyoxylate
-
-
-
?
IC50 Value
EC Number
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
4.1.3.1
0.002
-
in 20 mM sodium phosphate buffer (pH 7.0), with 3.75 mM MgCl2 and 4.1 mM phenylhydrazine, at 37°C
Magnaporthe grisea
2,2',3,3'-tetrabromo-4,4',5,5'-tetrahydroxydiphenylmethane
4.1.3.1
0.0021
-
in 20 mM sodium phosphate buffer (pH 7.0), with 3.75 mM MgCl2 and 4.1 mM phenylhydrazine, at 37°C
Magnaporthe grisea
3-bromo-4-(2,3-dibromo-4,5-dihydroxybenzyl)-5-methoxymethylpyrocatechol
4.1.3.1
0.0028
-
in 20 mM sodium phosphate buffer (pH 7.0), with 3.75 mM MgCl2 and 4.1 mM phenylhydrazine, at 37°C
Magnaporthe grisea
2,2',3-tribromo-3',4,4',5-tetrahydroxy-6'-hydroxymethyldiphenylmethane
4.1.3.1
0.0924
-
in 20 mM sodium phosphate buffer (pH 7.0), with 3.75 mM MgCl2 and 4.1 mM phenylhydrazine, at 37°C
Magnaporthe grisea
3-nitropropionate
4.1.3.1
0.0926
-
in 20 mM sodium phosphate buffer (pH 7.0), with 3.75 mM MgCl2 and 4.1 mM phenylhydrazine, at 37°C
Magnaporthe grisea
2,3-dibromo-4,5-dihydroxybenzyl alcohol
4.1.3.1
0.116
-
in 20 mM sodium phosphate buffer (pH 7.0), with 3.75 mM MgCl2 and 4.1 mM phenylhydrazine, at 37°C
Magnaporthe grisea
3,5-dibromo-4-hydroxyphenylethylamine
4.1.3.1
0.1256
-
in 20 mM sodium phosphate buffer (pH 7.0), with 3.75 mM MgCl2 and 4.1 mM phenylhydrazine, at 37°C
Magnaporthe grisea
2,3-dibromo-4,5-dihydroxybenzyl methyl ether
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
4.1.3.1
expressed in Escherichia coli strain BLR (DE3)
Magnaporthe grisea
IC50 Value (protein specific)
EC Number
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
4.1.3.1
0.002
-
in 20 mM sodium phosphate buffer (pH 7.0), with 3.75 mM MgCl2 and 4.1 mM phenylhydrazine, at 37°C
Magnaporthe grisea
2,2',3,3'-tetrabromo-4,4',5,5'-tetrahydroxydiphenylmethane
4.1.3.1
0.0021
-
in 20 mM sodium phosphate buffer (pH 7.0), with 3.75 mM MgCl2 and 4.1 mM phenylhydrazine, at 37°C
Magnaporthe grisea
3-bromo-4-(2,3-dibromo-4,5-dihydroxybenzyl)-5-methoxymethylpyrocatechol
4.1.3.1
0.0028
-
in 20 mM sodium phosphate buffer (pH 7.0), with 3.75 mM MgCl2 and 4.1 mM phenylhydrazine, at 37°C
Magnaporthe grisea
2,2',3-tribromo-3',4,4',5-tetrahydroxy-6'-hydroxymethyldiphenylmethane
4.1.3.1
0.0924
-
in 20 mM sodium phosphate buffer (pH 7.0), with 3.75 mM MgCl2 and 4.1 mM phenylhydrazine, at 37°C
Magnaporthe grisea
3-nitropropionate
4.1.3.1
0.0926
-
in 20 mM sodium phosphate buffer (pH 7.0), with 3.75 mM MgCl2 and 4.1 mM phenylhydrazine, at 37°C
Magnaporthe grisea
2,3-dibromo-4,5-dihydroxybenzyl alcohol
4.1.3.1
0.116
-
in 20 mM sodium phosphate buffer (pH 7.0), with 3.75 mM MgCl2 and 4.1 mM phenylhydrazine, at 37°C
Magnaporthe grisea
3,5-dibromo-4-hydroxyphenylethylamine
4.1.3.1
0.1256
-
in 20 mM sodium phosphate buffer (pH 7.0), with 3.75 mM MgCl2 and 4.1 mM phenylhydrazine, at 37°C
Magnaporthe grisea
2,3-dibromo-4,5-dihydroxybenzyl methyl ether
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
4.1.3.1
2,2',3,3'-tetrabromo-4,4',5,5'-tetrahydroxydiphenylmethane
-
Magnaporthe grisea
4.1.3.1
2,2',3-tribromo-3',4,4',5-tetrahydroxy-6'-hydroxymethyldiphenylmethane
-
Magnaporthe grisea
4.1.3.1
2,3-dibromo-4,5-dihydroxybenzyl alcohol
-
Magnaporthe grisea
4.1.3.1
2,3-dibromo-4,5-dihydroxybenzyl methyl ether
-
Magnaporthe grisea
4.1.3.1
3,5-dibromo-4-hydroxyphenylethylamine
-
Magnaporthe grisea
4.1.3.1
3-bromo-4-(2,3-dibromo-4,5-dihydroxybenzyl)-5-methoxymethylpyrocatechol
-
Magnaporthe grisea
4.1.3.1
3-nitropropionate
-
Magnaporthe grisea
4.1.3.1
additional information
not inhibited by bromopyruvate
Magnaporthe grisea
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.1.3.1
Ni-NTA column chromatography
Magnaporthe grisea
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.3.1
isocitrate
-
680310
Magnaporthe grisea
succinate + glyoxylate
-
-
-
?
4.1.3.1
isocitrate
-
680310
Magnaporthe grisea Guy 11
succinate + glyoxylate
-
-
-
?