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Literature summary extracted from

  • Hofinger, E.S.; Spickenreither, M.; Oschmann, J.; Bernhardt, G.; Rudolph, R.; Buschauer, A.
    Recombinant human hyaluronidase Hyal-1: insect cells versus Escherichia coli as expression system and identification of low molecular weight inhibitors (2007), Glycobiology, 17, 444-453.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.35 expressed in Escherichia coli BL21(DE3)RIL cells and Drosophila melanogaster Schneider-2 cells Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.35 glycyrrhizic acid
-
 Homo sapiens
3.2.1.35 L-Arginine-HCl 8% residual activity at 1 mM Homo sapiens
3.2.1.35 L-ascorbic acid decanoate
-
 Homo sapiens
3.2.1.35 L-ascorbic acid dodecanoate
-
 Homo sapiens
3.2.1.35 L-ascorbic acid tridecanoate
-
 Homo sapiens
3.2.1.35 L-ascorbic acid undecanoate
-
 Homo sapiens
3.2.1.35 NaCl complete inhibition at 1 mM Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.35 48300
-
 recombinant enzyme from Escherichia coli, SDS-PAGE Homo sapiens
3.2.1.35 54000
-
 recombinant enzyme from Drosophila melanogaster Schneider-2 cells, SDS-PAGE Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.35 Homo sapiens
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.35 glycoprotein
-
 Homo sapiens

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.35 ammonium sulfate precipitation and Ni-ion metal affinity chromatography Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.35 hyaluronan + 2 H2O
-
 Homo sapiens N-acetyl-beta-D-glucosamine + D-glucuronate + ?
-
 ?
3.2.1.35 hyaluronic acid + H2O
-
 Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.35 Hyal-1
-
 Homo sapiens
3.2.1.35 hyaluronidase
-
 Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.35 3.5 4
-
 Homo sapiens

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.2.1.35 0.026
-
 at pH 3.5 Homo sapiens glycyrrhizic acid
3.2.1.35 0.05
-
 at pH 3.5 Homo sapiens L-ascorbic acid tridecanoate
3.2.1.35 0.076
-
 at pH 3.5 Homo sapiens L-ascorbic acid dodecanoate
3.2.1.35 0.143
-
 at pH 3.5 Homo sapiens L-ascorbic acid undecanoate
3.2.1.35 0.378
-
 at pH 3.5 Homo sapiens L-ascorbic acid decanoate