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Literature summary extracted from

  • Kuokkanen, E.; Smith, W.; Maekinen, M.; Tuominen, H.; Puhka, M.; Jokitalo, E.; Duvet, S.; Berg, T.; Heikinheimo, P.
    Characterization and subcellular localization of human neutral class II alpha-mannosidase cytosolic enzymes/free oligosaccharides/glycosidehydrolase family 38/M2C1/N-glycosylation (2007), Glycobiology, 17, 1084-1093.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.114 additional information no effect with EDTA Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.114 preparation of expression construct containing 6 x His and tobacco etch virus protease recognition sites in the N-terminus of NAM and integrated into the Pichia pastoris chromosome under inducible alcohol oxygenase promoter. CHO-K1 cells transfected with pNAM-EGFP Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.114 Cu2+ completely inhibits Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.114 cytosol granularly distributed Homo sapiens 5829
-
3.2.1.114 additional information absent from the endoplasmic reticulum, lysosomes, and autophagosomes Homo sapiens
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.114 Co2+
-
 Homo sapiens
3.2.1.114 Fe2+ is more activated by Fe2+ than Co2+ Homo sapiens
3.2.1.114 Mn2+
-
 Homo sapiens
3.2.1.114 additional information no effect with Zn2+, Ca2+, and Mg2+ Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.114 112000
-
 4 * 112000, SDS-PAGE Homo sapiens
3.2.1.114 468000
-
 gel filtration Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.114 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.114 ammonium sulfate fractionation of recombinant enzyme followed by two cobalt chelating chromatography steps Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.114 fibroblast
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.114 (Man)9GlcNAc + H2O hydrolysis to (Man)5GlcNAc in the presence of Fe2+, Co2+, and Mn2+. Without activating cations the main reaction product is (Man)8GlcNAc Homo sapiens (Man)5GlcNAc + D-mannose
-
 ?
3.2.1.114 4-nitrophenyl alpha-D-mannoside + H2O
-
 Homo sapiens 4-nitrophenol + alpha-D-mannose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.114 tetramer 4 * 112000, SDS-PAGE Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
3.2.1.114 NAM
-
 Homo sapiens
3.2.1.114 neutral alpha-mannosidase
-
 Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.114 additional information
-
 optimal pH is between 6.25 and 6.75 Homo sapiens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.114 4.25 8
-
 Homo sapiens