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Literature summary extracted from

  • Fukamizo, T.; Fleury, A.; Cote, N.; Mitsutomi, M.; Brzezinski, R.
    Exo-beta-D-glucosaminidase from Amycolatopsis orientalis: catalytic residues, sugar recognition specificity, kinetics, and synergism (2006), Glycobiology, 16, 1064-1072.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.165 synthesis efficient tool for industrial production of glucosamine monosaccharide Amycolatopsis orientalis

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.165 D469A inactive mutant enzyme Amycolatopsis orientalis
3.2.1.165 D469E inactive mutant enzyme Amycolatopsis orientalis
3.2.1.165 E541D inactive mutant enzyme Amycolatopsis orientalis
3.2.1.165 E541Q inactive mutant enzyme Amycolatopsis orientalis
3.2.1.165 S468N/D469E inactive mutant enzyme Amycolatopsis orientalis

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.165 Amycolatopsis orientalis
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-
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.165 chitotetraose + H2O at first, the enzyme predominantly produces D-glucosamine and chitotriose, which is further degraded into D-glucosamine and chitobiose. The transglycosylation product, chitopentaose, is also produced, together with a lesser amount of chitohexaose Amycolatopsis orientalis D-glucosamine
-
 ?
3.2.1.165 GlcNbeta(1-4)GlcNbeta(1-4)GlcNbeta(1-4)GlcNAc + H2O (GlcN)3-GlcNAc (mono-N-acetylated chitotetraose, the reducing end residue is N-acetylated) is at first degraded into (GlcN)2-GlcNAc, and then into GlcN-GlcNAc. After a longer incubation period, the substrate is finally hydrolyzed into monosaccharides, GlcN and GlcNAc Amycolatopsis orientalis GlcNbeta(1-4)GlcNbeta(1-4)GlcNAc + D-glucosamine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.165 exo-beta-D-glucosaminidase
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 Amycolatopsis orientalis