BRENDA - Enzyme Database

A glycolytic enzyme, enolase, is recruited as a cofactor of tRNA targeting toward mitochondria in Saccharomyces cerevisiae

Entelis, N.; Brandina, I.; Kamenski, P.; Krasheninnikov, I.A.; Martin, R.P.; Tarassov, I.; Genes Dev. 20, 1609-1620 (2006)

Data extracted from this reference:

Application
EC Number
Application
Commentary
Organism
4.2.1.11
molecular biology
studies on mitochondrial import machinery of Saccharomyces cerevisiae
Saccharomyces cerevisiae
Cloned(Commentary)
EC Number
Commentary
Organism
4.2.1.11
over-expression in Escherichia coli strain BL21, expression vector pET3a, His-tagged recombinant protein
Saccharomyces cerevisiae
Engineering
EC Number
Amino acid exchange
Commentary
Organism
4.2.1.11
additional information
engineered enolase forms disrupted in catalytic activity retain features to direct mitochondrial import of tRNA
Saccharomyces cerevisiae
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
4.2.1.11
mitochondrion
up to 7% of enolase activity associated with mitochondria, targeting towards the mitochondrial outer membrane, affinity to the mitochondrial surface more than 10fold higher for Eno2p than for Eno1p
Saccharomyces cerevisiae
5739
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.11
49000
-
gel electrophoresis
Saccharomyces cerevisiae
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.2.1.11
2-phospho-D-glycerate
Saccharomyces cerevisiae
-
phosphoenolpyruvate + H2O
-
-
r
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.2.1.11
Saccharomyces cerevisiae
-
W303
-
Purification (Commentary)
EC Number
Commentary
Organism
4.2.1.11
gel filtration, for in vitro import assays into mitochondria and three-hybrid analysis
Saccharomyces cerevisiae
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.2.1.11
additional information
-
additional and independent function beyond glycolytic enzyme function, involvement in mitochondrial tRNA targeting, depletion of enolase inhibits tRNA import in vivo, activity of enolase as an alternative molecular chaperone suggested
Saccharomyces cerevisiae
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.11
2-phospho-D-glycerate
-
680008
Saccharomyces cerevisiae
phosphoenolpyruvate + H2O
-
-
-
r
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
4.2.1.11
additional information
specific affinity to tRNA imported into mitochondria, involved to direct mitochondrial tRNA import in vitro
Saccharomyces cerevisiae
Application (protein specific)
EC Number
Application
Commentary
Organism
4.2.1.11
molecular biology
studies on mitochondrial import machinery of Saccharomyces cerevisiae
Saccharomyces cerevisiae
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.11
over-expression in Escherichia coli strain BL21, expression vector pET3a, His-tagged recombinant protein
Saccharomyces cerevisiae
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
4.2.1.11
additional information
specific affinity to tRNA imported into mitochondria, involved to direct mitochondrial tRNA import in vitro
Saccharomyces cerevisiae
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
4.2.1.11
additional information
engineered enolase forms disrupted in catalytic activity retain features to direct mitochondrial import of tRNA
Saccharomyces cerevisiae
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
4.2.1.11
mitochondrion
up to 7% of enolase activity associated with mitochondria, targeting towards the mitochondrial outer membrane, affinity to the mitochondrial surface more than 10fold higher for Eno2p than for Eno1p
Saccharomyces cerevisiae
5739
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.11
49000
-
gel electrophoresis
Saccharomyces cerevisiae
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.2.1.11
2-phospho-D-glycerate
Saccharomyces cerevisiae
-
phosphoenolpyruvate + H2O
-
-
r
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.11
gel filtration, for in vitro import assays into mitochondria and three-hybrid analysis
Saccharomyces cerevisiae
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.2.1.11
additional information
-
additional and independent function beyond glycolytic enzyme function, involvement in mitochondrial tRNA targeting, depletion of enolase inhibits tRNA import in vivo, activity of enolase as an alternative molecular chaperone suggested
Saccharomyces cerevisiae
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.11
2-phospho-D-glycerate
-
680008
Saccharomyces cerevisiae
phosphoenolpyruvate + H2O
-
-
-
r