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Literature summary extracted from

  • Entelis, N.; Brandina, I.; Kamenski, P.; Krasheninnikov, I.A.; Martin, R.P.; Tarassov, I.
    A glycolytic enzyme, enolase, is recruited as a cofactor of tRNA targeting toward mitochondria in Saccharomyces cerevisiae (2006), Genes Dev., 20, 1609-1620.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
4.2.1.11 molecular biology studies on mitochondrial import machinery of Saccharomyces cerevisiae Saccharomyces cerevisiae

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.11 over-expression in Escherichia coli strain BL21, expression vector pET3a, His-tagged recombinant protein Saccharomyces cerevisiae

Protein Variants

EC Number Protein Variants Comment Organism
4.2.1.11 additional information engineered enolase forms disrupted in catalytic activity retain features to direct mitochondrial import of tRNA Saccharomyces cerevisiae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.2.1.11 mitochondrion up to 7% of enolase activity associated with mitochondria, targeting towards the mitochondrial outer membrane, affinity to the mitochondrial surface more than 10fold higher for Eno2p than for Eno1p Saccharomyces cerevisiae 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.11 49000
-
 gel electrophoresis Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.11 2-phospho-D-glycerate Saccharomyces cerevisiae
-
 phosphoenolpyruvate + H2O
-
 r

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.11 Saccharomyces cerevisiae
-
 W303
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.11 gel filtration, for in vitro import assays into mitochondria and three-hybrid analysis Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.11 additional information
-
 additional and independent function beyond glycolytic enzyme function, involvement in mitochondrial tRNA targeting, depletion of enolase inhibits tRNA import in vivo, activity of enolase as an alternative molecular chaperone suggested Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.11 2-phospho-D-glycerate
-
 Saccharomyces cerevisiae phosphoenolpyruvate + H2O
-
 r

Synonyms

EC Number Synonyms Comment Organism
4.2.1.11 enolase
-
 Saccharomyces cerevisiae

Cofactor

EC Number Cofactor Comment Organism Structure
4.2.1.11 additional information specific affinity to tRNA imported into mitochondria, involved to direct mitochondrial tRNA import in vitro Saccharomyces cerevisiae