BRENDA - Enzyme Database

Cloning and characterization of DPPL1 and DPPL2, representatives of a novel type of mammalian phosphatidate phosphatase

Takeuchi, M.; Harigai, M.; Momohara, S.; Ball, E.; Abe, J.; Furuichi, K.; Kamatani, N.; Gene 399, 174-180 (2007)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
3.1.3.4
expression of isozyme DPPL1 in Spodoptera frugiperda Sf9 cells; expression of isozyme DPPL2 in Spodoptera frugiperda Sf9 cells
Homo sapiens
3.1.3.81
expression of isozyme DPPL1 in Spodoptera frugiperda Sf9 cells; expression of isozyme DPPL2 in Spodoptera frugiperda Sf9 cells
Homo sapiens
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.3.4
NEM
inhibition of isozyme DPPL1; inhibition of isozyme DPPL2
Homo sapiens
3.1.3.81
NEM
inhibition of isozyme DPPL1; inhibition of isozyme DPPL2
Homo sapiens
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.3.4
0.041
-
diacylglycerol diphosphate
pH 6.5, 37C, recombinant isozyme DPPL1
Homo sapiens
3.1.3.4
0.104
-
diacylglycerol diphosphate
pH 6.5, 37C, recombinant isozyme DPPL2
Homo sapiens
3.1.3.4
0.277
-
phosphatidic acid
pH 6.5, 37C, recombinant isozyme DPPL1
Homo sapiens
3.1.3.4
0.295
-
lysophosphatidic acid
pH 6.5, 37C, recombinant isozyme DPPL1
Homo sapiens
3.1.3.4
0.506
-
phosphatidic acid
pH 6.5, 37C, recombinant isozyme DPPL2
Homo sapiens
3.1.3.4
0.58
-
lysophosphatidic acid
pH 6.5, 37C, recombinant isozyme DPPL2
Homo sapiens
3.1.3.81
0.041
-
diacylglycerol diphosphate
pH 6.5, 37C, recombinant isozyme DPPL2
Homo sapiens
3.1.3.81
0.104
-
diacylglycerol diphosphate
pH 6.5, 37C, recombinant isozyme DPPL1
Homo sapiens
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.3.4
additional information
isozyme DPPL1 is Mg2+-independent; isozyme DPPL2 is Mg2+-independent
Homo sapiens
3.1.3.81
additional information
isozyme DPPL1 is Mg2+-independent; isozyme DPPL2 is Mg2+-independent
Homo sapiens
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.3.4
Homo sapiens
Q5VZY2
DPPL2; isozyme DPPL2
-
3.1.3.4
Homo sapiens
Q8NEB5
DPPL1; isozyme DPPL1
-
3.1.3.81
Homo sapiens
Q5VZY2
DPPL2; isozyme DPPL2
-
3.1.3.81
Homo sapiens
Q8NEB5
DPPL1; isozyme DPPL1
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.3.4
A-549 cell
-
Homo sapiens
-
3.1.3.4
blood vessel
synovium containing, specific expression of DPPL2
Homo sapiens
-
3.1.3.4
brain
isozyme DPPL2
Homo sapiens
-
3.1.3.4
endothelial cell
isozyme DPPL2
Homo sapiens
-
3.1.3.4
endothelium
specific expression of DPPL2, expression in endothelial cell lines, overview
Homo sapiens
-
3.1.3.4
IM-9 cell
-
Homo sapiens
-
3.1.3.4
JURKAT cell
-
Homo sapiens
-
3.1.3.4
kidney
isozyme DPPL2
Homo sapiens
-
3.1.3.4
MCF-7 cell
-
Homo sapiens
-
3.1.3.4
additional information
DPPL1 is ubiquitously expressed in various tissues and cells, overview; DPPL2 mRNA is restricted to several tissues, expression in cancer cell lines, overview
Homo sapiens
-
3.1.3.4
PC-3 cell
-
Homo sapiens
-
3.1.3.4
plasma cell
specific expression of DPPL2
Homo sapiens
-
3.1.3.4
T-lymphocyte
specific expression of DPPL2
Homo sapiens
-
3.1.3.4
testis
isozyme DPPL2
Homo sapiens
-
3.1.3.4
THP-1 cell
-
Homo sapiens
-
3.1.3.4
WiDr cell
-
Homo sapiens
-
3.1.3.81
A-549 cell
-
Homo sapiens
-
3.1.3.81
blood vessel
synovium containing, specific expression of isozyme DPPL2
Homo sapiens
-
3.1.3.81
brain
isozyme DPPL2
Homo sapiens
-
3.1.3.81
endothelial cell
isozyme DPPL2
Homo sapiens
-
3.1.3.81
endothelium
specific expression of DPPL2, expression in endothelial cell lines, overview
Homo sapiens
-
3.1.3.81
IM-9 cell
-
Homo sapiens
-
3.1.3.81
JURKAT cell
-
Homo sapiens
-
3.1.3.81
kidney
isozyme DPPL2
Homo sapiens
-
3.1.3.81
MCF-7 cell
-
Homo sapiens
-
3.1.3.81
additional information
DPPL1 is ubiquitously expressed in various tissues and cells, overview; DPPL2 mRNA is restricted to several tissues, expression in cancer cell lines, overview
Homo sapiens
-
3.1.3.81
PC-3 cell
-
Homo sapiens
-
3.1.3.81
plasma cell
specific expression of isozyme DPPL2
Homo sapiens
-
3.1.3.81
T-lymphocyte
specific expression of isozyme DPPL2
Homo sapiens
-
3.1.3.81
testis
isozyme DPPL2
Homo sapiens
-
3.1.3.81
THP-1 cell
-
Homo sapiens
-
3.1.3.81
WiDr cell
-
Homo sapiens
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.3.4
diacylglycerol diphosphate + H2O
preferred substrate of isozyme DPPL1
679998
Homo sapiens
?
-
-
-
?
3.1.3.4
diacylglycerol diphosphate + H2O
preferred substrate of isozyme DPPL2
679998
Homo sapiens
?
-
-
-
?
3.1.3.4
lysophosphatidic acid + H2O
-
679998
Homo sapiens
?
-
-
-
?
3.1.3.4
phosphatidic acid + H2O
-
679998
Homo sapiens
1,2-diacyl-sn-glycerol + phosphate
-
-
-
?
3.1.3.81
diacylglycerol diphosphate + H2O
preferred substrate of isozyme DPPL1
679998
Homo sapiens
diacylglycerol phosphate + phosphate
-
-
-
?
3.1.3.81
diacylglycerol diphosphate + H2O
preferred substrate of isozyme DPPL2
679998
Homo sapiens
diacylglycerol phosphate + phosphate
-
-
-
?
Temperature Optimum [C]
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
3.1.3.4
37
-
assay at; assay at
Homo sapiens
3.1.3.81
37
-
assay at; assay at
Homo sapiens
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.3.4
6.5
-
assay at; assay at
Homo sapiens
3.1.3.81
6.5
-
assay at; assay at
Homo sapiens
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.3.4
expression of isozyme DPPL1 in Spodoptera frugiperda Sf9 cells
Homo sapiens
3.1.3.4
expression of isozyme DPPL2 in Spodoptera frugiperda Sf9 cells
Homo sapiens
3.1.3.81
expression of isozyme DPPL1 in Spodoptera frugiperda Sf9 cells
Homo sapiens
3.1.3.81
expression of isozyme DPPL2 in Spodoptera frugiperda Sf9 cells
Homo sapiens
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.3.4
NEM
inhibition of isozyme DPPL2
Homo sapiens
3.1.3.4
NEM
inhibition of isozyme DPPL1
Homo sapiens
3.1.3.81
NEM
inhibition of isozyme DPPL2
Homo sapiens
3.1.3.81
NEM
inhibition of isozyme DPPL1
Homo sapiens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.3.4
0.041
-
diacylglycerol diphosphate
pH 6.5, 37C, recombinant isozyme DPPL1
Homo sapiens
3.1.3.4
0.104
-
diacylglycerol diphosphate
pH 6.5, 37C, recombinant isozyme DPPL2
Homo sapiens
3.1.3.4
0.277
-
phosphatidic acid
pH 6.5, 37C, recombinant isozyme DPPL1
Homo sapiens
3.1.3.4
0.295
-
lysophosphatidic acid
pH 6.5, 37C, recombinant isozyme DPPL1
Homo sapiens
3.1.3.4
0.506
-
phosphatidic acid
pH 6.5, 37C, recombinant isozyme DPPL2
Homo sapiens
3.1.3.4
0.58
-
lysophosphatidic acid
pH 6.5, 37C, recombinant isozyme DPPL2
Homo sapiens
3.1.3.81
0.041
-
diacylglycerol diphosphate
pH 6.5, 37C, recombinant isozyme DPPL2
Homo sapiens
3.1.3.81
0.104
-
diacylglycerol diphosphate
pH 6.5, 37C, recombinant isozyme DPPL1
Homo sapiens
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.3.4
additional information
isozyme DPPL2 is Mg2+-independent
Homo sapiens
3.1.3.4
additional information
isozyme DPPL1 is Mg2+-independent
Homo sapiens
3.1.3.81
additional information
isozyme DPPL2 is Mg2+-independent
Homo sapiens
3.1.3.81
additional information
isozyme DPPL1 is Mg2+-independent
Homo sapiens
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.3.4
A-549 cell
-
Homo sapiens
-
3.1.3.4
blood vessel
synovium containing, specific expression of DPPL2
Homo sapiens
-
3.1.3.4
brain
isozyme DPPL2
Homo sapiens
-
3.1.3.4
endothelial cell
isozyme DPPL2
Homo sapiens
-
3.1.3.4
endothelium
specific expression of DPPL2, expression in endothelial cell lines, overview
Homo sapiens
-
3.1.3.4
IM-9 cell
-
Homo sapiens
-
3.1.3.4
JURKAT cell
-
Homo sapiens
-
3.1.3.4
kidney
isozyme DPPL2
Homo sapiens
-
3.1.3.4
MCF-7 cell
-
Homo sapiens
-
3.1.3.4
additional information
DPPL2 mRNA is restricted to several tissues, expression in cancer cell lines, overview
Homo sapiens
-
3.1.3.4
additional information
DPPL1 is ubiquitously expressed in various tissues and cells, overview
Homo sapiens
-
3.1.3.4
PC-3 cell
-
Homo sapiens
-
3.1.3.4
plasma cell
specific expression of DPPL2
Homo sapiens
-
3.1.3.4
T-lymphocyte
specific expression of DPPL2
Homo sapiens
-
3.1.3.4
testis
isozyme DPPL2
Homo sapiens
-
3.1.3.4
THP-1 cell
-
Homo sapiens
-
3.1.3.4
WiDr cell
-
Homo sapiens
-
3.1.3.81
A-549 cell
-
Homo sapiens
-
3.1.3.81
blood vessel
synovium containing, specific expression of isozyme DPPL2
Homo sapiens
-
3.1.3.81
brain
isozyme DPPL2
Homo sapiens
-
3.1.3.81
endothelial cell
isozyme DPPL2
Homo sapiens
-
3.1.3.81
endothelium
specific expression of DPPL2, expression in endothelial cell lines, overview
Homo sapiens
-
3.1.3.81
IM-9 cell
-
Homo sapiens
-
3.1.3.81
JURKAT cell
-
Homo sapiens
-
3.1.3.81
kidney
isozyme DPPL2
Homo sapiens
-
3.1.3.81
MCF-7 cell
-
Homo sapiens
-
3.1.3.81
additional information
DPPL2 mRNA is restricted to several tissues, expression in cancer cell lines, overview
Homo sapiens
-
3.1.3.81
additional information
DPPL1 is ubiquitously expressed in various tissues and cells, overview
Homo sapiens
-
3.1.3.81
PC-3 cell
-
Homo sapiens
-
3.1.3.81
plasma cell
specific expression of isozyme DPPL2
Homo sapiens
-
3.1.3.81
T-lymphocyte
specific expression of isozyme DPPL2
Homo sapiens
-
3.1.3.81
testis
isozyme DPPL2
Homo sapiens
-
3.1.3.81
THP-1 cell
-
Homo sapiens
-
3.1.3.81
WiDr cell
-
Homo sapiens
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.3.4
diacylglycerol diphosphate + H2O
preferred substrate of isozyme DPPL1
679998
Homo sapiens
?
-
-
-
?
3.1.3.4
diacylglycerol diphosphate + H2O
preferred substrate of isozyme DPPL2
679998
Homo sapiens
?
-
-
-
?
3.1.3.4
lysophosphatidic acid + H2O
-
679998
Homo sapiens
?
-
-
-
?
3.1.3.4
phosphatidic acid + H2O
-
679998
Homo sapiens
1,2-diacyl-sn-glycerol + phosphate
-
-
-
?
3.1.3.81
diacylglycerol diphosphate + H2O
preferred substrate of isozyme DPPL1
679998
Homo sapiens
diacylglycerol phosphate + phosphate
-
-
-
?
3.1.3.81
diacylglycerol diphosphate + H2O
preferred substrate of isozyme DPPL2
679998
Homo sapiens
diacylglycerol phosphate + phosphate
-
-
-
?
Temperature Optimum [C] (protein specific)
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
3.1.3.4
37
-
assay at
Homo sapiens
3.1.3.81
37
-
assay at
Homo sapiens
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.3.4
6.5
-
assay at
Homo sapiens
3.1.3.81
6.5
-
assay at
Homo sapiens