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Literature summary extracted from

  • Bhatti, H.N.; Rashid, M.H.; Nawaz, R.; Asgher, M.; Perveen, R.; Jabbar, A.
    Purification and characterization of a novel glucoamylase from Fusarium solani (2007), Food Chem., 103, 338-343.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.3 Fe3+
-
 Fusarium solani
3.2.1.3 Hg2+ strong inhibition Fusarium solani
3.2.1.3 Ni2+
-
 Fusarium solani
3.2.1.3 Pb2+ strong inhibition Fusarium solani
3.2.1.3 Zn2+ strong inhibition Fusarium solani

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.3 additional information
-
 additional information
-
 Fusarium solani
3.2.1.3 1.9
-
 soluble starch pH 4.5, 40°C, purified native enzyme Fusarium solani

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.3 extracellular
-
 Fusarium solani
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.3 Cu2+ slight activation Fusarium solani
3.2.1.3 Mg2+ slight activation Fusarium solani

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.3 40000
-
 1 * 40000, SDS-PAGE Fusarium solani
3.2.1.3 41000
-
 gel filtration Fusarium solani

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.3 starch + H2O Fusarium solani important industrial enzyme that removes the glucose units from the non-reducing chain-ends of starch and glycogen by hydrolyzing alpha-1,4 linkages consecutively D-glucose + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.3 Fusarium solani
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.3 native extracellular enzyme 26.2fold to homogeneity by ammonium sulfate precipitation, anion exchange chromatography, and hydrophobic interaction chromatography, followed by gel filtration Fusarium solani

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.3 additional information
-
-
 Fusarium solani

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.3 soluble starch + H2O
-
 Fusarium solani beta-D-glucose + ?
-
 ?
3.2.1.3 soluble starch + H2O
-
 Fusarium solani beta-D-glucose + soluble starch
-
 ?
3.2.1.3 starch + H2O soluble starch Fusarium solani D-glucose + ?
-
 ?
3.2.1.3 starch + H2O important industrial enzyme that removes the glucose units from the non-reducing chain-ends of starch and glycogen by hydrolyzing alpha-1,4 linkages consecutively Fusarium solani D-glucose + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.3 monomer 1 * 40000, SDS-PAGE Fusarium solani

Synonyms

EC Number Synonyms Comment Organism
3.2.1.3 glucoamylase
-
 Fusarium solani

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.3 40
-
 assay at Fusarium solani

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.3 30 60
-
 Fusarium solani

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.3 50
-
 purified native enzyme, 79% remaining activity after 60 min Fusarium solani
3.2.1.3 60
-
 purified native enzyme, half-life is 26 min Fusarium solani

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.3 7.35
-
 soluble starch pH 4.5, 40°C, purified native enzyme Fusarium solani

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.3 4.5
-
-
 Fusarium solani

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.3 2 9
-
 Fusarium solani