BRENDA - Enzyme Database

Identification of the aceA gene encoding isocitrate lyase required for the growth of Pseudomonas aeruginosa on acetate, acyclic terpenes and leucine

Diaz-Perez, A.L.; Roman-Doval, C.; Diaz-Perez, C.; Cervantes, C.; Sosa-Aguirre, C.R.; Lopez-Meza, J.E.; Campos-Garcia, J.; FEMS Microbiol. Lett. 269, 309-316 (2007)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
4.1.3.1
expressed in Escherichia coli
Pseudomonas aeruginosa
Engineering
EC Number
Amino acid exchange
Commentary
Organism
4.1.3.1
additional information
transposition mutant PAO1SM::Himar1GmR is unable to grow on citronellol, n-octanol, leucine and acetate, and shows totally abolished ICL activity
Pseudomonas aeruginosa
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.1.3.1
60000
-
SDS-PAGE
Pseudomonas aeruginosa
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.5.4
additional information
Pseudomonas aeruginosa
a mutant with an interrupted putative mqo gene, in which malate:quinone oxidoreductase, an enzyme involved in the citric acid cycle/glyoxylate cycle, is defective, shows a severe growth defect on ethanol and is unable to grow on acetate
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.5.4
Pseudomonas aeruginosa
Q9HVF1
ATCC 17933
-
4.1.3.1
Pseudomonas aeruginosa
Q9I0K4
-
-
Purification (Commentary)
EC Number
Commentary
Organism
4.1.3.1
Macro-Prep High Q Support column chromatography
Pseudomonas aeruginosa
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.5.4
additional information
a mutant with an interrupted putative mqo gene, in which malate:quinone oxidoreductase, an enzyme involved in the citric acid cycle/glyoxylate cycle, is defective, shows a severe growth defect on ethanol and is unable to grow on acetate
679906
Pseudomonas aeruginosa
?
-
-
-
-
4.1.3.1
isocitrate
-
679906
Pseudomonas aeruginosa
succinate + glyoxylate
-
-
-
?
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
4.1.3.1
expressed in Escherichia coli
Pseudomonas aeruginosa
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
4.1.3.1
additional information
transposition mutant PAO1SM::Himar1GmR is unable to grow on citronellol, n-octanol, leucine and acetate, and shows totally abolished ICL activity
Pseudomonas aeruginosa
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.1.3.1
60000
-
SDS-PAGE
Pseudomonas aeruginosa
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.5.4
additional information
Pseudomonas aeruginosa
a mutant with an interrupted putative mqo gene, in which malate:quinone oxidoreductase, an enzyme involved in the citric acid cycle/glyoxylate cycle, is defective, shows a severe growth defect on ethanol and is unable to grow on acetate
?
-
-
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.1.3.1
Macro-Prep High Q Support column chromatography
Pseudomonas aeruginosa
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.5.4
additional information
a mutant with an interrupted putative mqo gene, in which malate:quinone oxidoreductase, an enzyme involved in the citric acid cycle/glyoxylate cycle, is defective, shows a severe growth defect on ethanol and is unable to grow on acetate
679906
Pseudomonas aeruginosa
?
-
-
-
-
4.1.3.1
isocitrate
-
679906
Pseudomonas aeruginosa
succinate + glyoxylate
-
-
-
?