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Literature summary extracted from
- A novel thermostable esterase from the thermoacidophilic archaeon Sulfolobus tokodaii strain 7. (2004), FEMS Microbiol. Lett., 236, 97-102.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.1.1.1 | expressed as a fusion protein in Escherichia coli | Sulfurisphaera tokodaii |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.1.1 | 0.53 | - |
4-nitrophenyl butyrate | - |
Sulfurisphaera tokodaii |  |
| 3.1.1.1 | 0.59 | - |
4-nitrophenyl hexanoate | - |
Sulfurisphaera tokodaii | |
| 3.1.1.1 | 0.63 | - |
4-nitrophenyl octanoate | - |
Sulfurisphaera tokodaii | |
| 3.1.1.1 | 25.2 | - |
4-nitrophenyl octanoate | pH 7.5, 70°C | Sulfurisphaera tokodaii | |
| 3.1.1.1 | 44.7 | - |
4-nitrophenyl hexanoate | pH 7.5, 70°C | Sulfurisphaera tokodaii | |
| 3.1.1.1 | 127 | - |
4-nitrophenyl butanoate | pH 7.5, 70°C | Sulfurisphaera tokodaii | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.1 | 33810 | - |
x * 33810, calculated from sequence | Sulfurisphaera tokodaii |
| 3.1.1.1 | 37000 | - |
x * 37000 (including a 3 kDa size increase by fusion tag), SDS-PAGE | Sulfurisphaera tokodaii |
Organic Solvent Stability
| EC Number | Organic Solvent | Comment | Organism |
|---|---|---|---|
| 3.1.1.1 | 1,4-dioxane | 30%, about 60% inhibition, at 70°C, in 50 mM HEPES (pH 7.5) using 4-nitrophenyl butanoate as the substrate | Sulfurisphaera tokodaii |
| 3.1.1.1 | acetonitrile | 30%, about 50% inhibition, at 70°C, in 50 mM HEPES (pH 7.5) using 4-nitrophenyl butanoate as the substrate | Sulfurisphaera tokodaii |
| 3.1.1.1 | dimethyl sulfoxide | 30%, anout 90% inhibition, at 70°C, in 50 mM HEPES (pH 7.5) using 4-nitrophenyl butanoate as the substrate | Sulfurisphaera tokodaii |
| 3.1.1.1 | tert-butyl alcohol | 20%, about 90% inhibition, at 70°C, in 50 mM HEPES (pH 7.5) using 4-nitrophenyl butanoate as the substrate | Sulfurisphaera tokodaii |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.1.1 | Sulfurisphaera tokodaii | Q976W8 | - |
- |
| 3.1.1.1 | Sulfurisphaera tokodaii 7 | Q976W8 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.1.1 | - |
Sulfurisphaera tokodaii |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.1 | 43.2 | - |
- |
Sulfurisphaera tokodaii |
| 3.1.1.1 | 43.2 | - |
pH 7.5, 70°C | Sulfurisphaera tokodaii |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.1.1 | 4-nitrophenyl butanoate + H2O | - |
Sulfurisphaera tokodaii | 4-nitrophenol + butanoate | - |
? | |
| 3.1.1.1 | 4-nitrophenyl butyrate + H2O | - |
Sulfurisphaera tokodaii | 4-nitrophenol + butyrate | - |
? | |
| 3.1.1.1 | 4-nitrophenyl butyrate + H2O | - |
Sulfurisphaera tokodaii 7 | 4-nitrophenol + butyrate | - |
? | |
| 3.1.1.1 | 4-nitrophenyl hexanoate + H2O | 31.6% of the activity with 4-nitrophenyl butyrate | Sulfurisphaera tokodaii | 4-nitrophenol + hexanoate | - |
? | |
| 3.1.1.1 | 4-nitrophenyl hexanoate + H2O | 31.5% of the activity compared to 4-nitrophenyl butyrate | Sulfurisphaera tokodaii | 4-nitrophenol + hexanoate | - |
? | |
| 3.1.1.1 | 4-nitrophenyl hexanoate + H2O | 31.6% of the activity with 4-nitrophenyl butyrate | Sulfurisphaera tokodaii 7 | 4-nitrophenol + hexanoate | - |
? | |
| 3.1.1.1 | 4-nitrophenyl hexanoate + H2O | 31.5% of the activity compared to 4-nitrophenyl butyrate | Sulfurisphaera tokodaii 7 | 4-nitrophenol + hexanoate | - |
? | |
| 3.1.1.1 | 4-nitrophenyl octanoate + H2O | 16.7% of the activity with 4-nitrophenyl butyrate | Sulfurisphaera tokodaii | 4-nitrophenol + octanoate | - |
? | |
| 3.1.1.1 | 4-nitrophenyl octanoate + H2O | 16.7% of the activity compared to 4-nitrophenyl butyrate | Sulfurisphaera tokodaii | 4-nitrophenol + octanoate | - |
? | |
| 3.1.1.1 | 4-nitrophenyl octanoate + H2O | 16.7% of the activity with 4-nitrophenyl butyrate | Sulfurisphaera tokodaii 7 | 4-nitrophenol + octanoate | - |
? | |
| 3.1.1.1 | 4-nitrophenyl octanoate + H2O | 16.7% of the activity compared to 4-nitrophenyl butyrate | Sulfurisphaera tokodaii 7 | 4-nitrophenol + octanoate | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.1.1.1 | ? | x * 33810, calculated from sequence | Sulfurisphaera tokodaii |
| 3.1.1.1 | ? | x * 37000 (including a 3 kDa size increase by fusion tag), SDS-PAGE | Sulfurisphaera tokodaii |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.1.1 | STK_00710 | locus name | Sulfurisphaera tokodaii |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.1 | 70 | - |
- |
Sulfurisphaera tokodaii |
| 3.1.1.1 | 70 | - |
substrate: 4-nitrophenyl butyrate, 4-nitrophenyl hexanoate or 4-nitrophenyl octanoate | Sulfurisphaera tokodaii |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.1 | 50 | 75 | 50°C: about 50% of maximal activity, 75°C: about 90% of maximal activity | Sulfurisphaera tokodaii |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.1 | 80 | - |
pH 7.5, 2 h, completely stable | Sulfurisphaera tokodaii |
| 3.1.1.1 | 80 | - |
2 h, retains 10% of its activity | Sulfurisphaera tokodaii |
| 3.1.1.1 | 85 | - |
half-life: 40 min | Sulfurisphaera tokodaii |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.1.1 | 25.2 | - |
4-nitrophenyl octanoate | - |
Sulfurisphaera tokodaii | |
| 3.1.1.1 | 25.2 | - |
4-nitrophenyl octanoate | pH 7.5, 70°C | Sulfurisphaera tokodaii | |
| 3.1.1.1 | 44.7 | - |
4-nitrophenyl hexanoate | - |
Sulfurisphaera tokodaii | |
| 3.1.1.1 | 44.7 | - |
4-nitrophenyl hexanoate | pH 7.5, 70°C | Sulfurisphaera tokodaii | |
| 3.1.1.1 | 127 | - |
4-nitrophenyl butyrate | - |
Sulfurisphaera tokodaii | |
| 3.1.1.1 | 127 | - |
4-nitrophenyl butanoate | pH 7.5, 70°C | Sulfurisphaera tokodaii | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.1 | 7.5 | 8 | - |
Sulfurisphaera tokodaii |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.1 | 4 | 9 | pH 4.0: about 60% of maximal activity, pH 9.0: about 80% of maximal activity | Sulfurisphaera tokodaii |
| 3.1.1.1 | 4 | 9 | pH 4.0: about 55% of maximal activity, pH 9.0: about 80% of maximal activity | Sulfurisphaera tokodaii |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.1.1 | 25.2 | - |
4-nitrophenyl octanoate | pH 7.5, 70°C | Sulfurisphaera tokodaii | |
| 3.1.1.1 | 44.7 | - |
4-nitrophenyl hexanoate | pH 7.5, 70°C | Sulfurisphaera tokodaii | |
| 3.1.1.1 | 127 | - |
4-nitrophenyl butanoate | pH 7.5, 70°C | Sulfurisphaera tokodaii | |
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