EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.1.1.3 | SDS | 8fold activation at 0.05%, no activation at 0.4% | Priestia megaterium |
EC Number | Cloned (Comment) | Organism |
---|---|---|
3.1.1.3 | gene lipA, DNA and amino acid sequence determination and analysis, expression in Escherichia coli 5K, sequence comparisons | Priestia megaterium |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.1.1.3 | Ag+ | 48% inhibition at 1 mM | Priestia megaterium | |
3.1.1.3 | EDTA | 60% inhibition at 20 mM, poor inhibition at 1 mM | Priestia megaterium | |
3.1.1.3 | Hg2+ | complete inhibition at 1 mM | Priestia megaterium | |
3.1.1.3 | phytic acid | strong inhibition | Priestia megaterium | |
3.1.1.3 | PMSF | strong inhibition | Priestia megaterium | |
3.1.1.3 | Triton X-100 | inhibition at concentrations above 1% | Priestia megaterium | |
3.1.1.3 | Urea | strong inhibition | Priestia megaterium |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.1.1.3 | extracellular | the enzyme is secreted | Priestia megaterium | - |
- |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.1.3 | Priestia megaterium | Q8RJP5 | precursor; precursor strain 370, gene lipA | - |
3.1.1.3 | Priestia megaterium ATCC 9885 | Q8RJP5 | precursor; precursor strain 370, gene lipA | - |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
3.1.1.3 | proteolytic modification | the enzyme is expressed as precursor protein | Priestia megaterium |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.1.1.3 | culture condition:rhodamine B-grown cell | - |
Priestia megaterium | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.1.3 | 4-methylumbelliferyl butyrate + H2O | - |
Priestia megaterium | 4-methylumbelliferol + butyrate | - |
? | |
3.1.1.3 | 4-methylumbelliferyl butyrate + H2O | - |
Priestia megaterium ATCC 9885 | 4-methylumbelliferol + butyrate | - |
? | |
3.1.1.3 | 4-nitrophenyl butyrate + H2O | high activity | Priestia megaterium | 4-nitrophenol + butyrate | - |
? | |
3.1.1.3 | 4-nitrophenyl butyrate + H2O | high activity | Priestia megaterium ATCC 9885 | 4-nitrophenol + butyrate | - |
? | |
3.1.1.3 | 4-nitrophenyl caprate + H2O | - |
Priestia megaterium | 4-nitrophenol + caprate | - |
? | |
3.1.1.3 | 4-nitrophenyl caproate + H2O | - |
Priestia megaterium | 4-nitrophenol + caproate | - |
? | |
3.1.1.3 | 4-nitrophenyl caproate + H2O | - |
Priestia megaterium ATCC 9885 | 4-nitrophenol + caproate | - |
? | |
3.1.1.3 | 4-nitrophenyl caprylate + H2O | - |
Priestia megaterium | 4-nitrophenol + caprylate | - |
? | |
3.1.1.3 | 4-nitrophenyl laurate + H2O | - |
Priestia megaterium | 4-nitrophenol + laurate | - |
? | |
3.1.1.3 | 4-nitrophenyl valerate + H2O | - |
Priestia megaterium | 4-nitrophenol + valerate | - |
? | |
3.1.1.3 | 4-nitrophenyl valerate + H2O | - |
Priestia megaterium ATCC 9885 | 4-nitrophenol + valerate | - |
? | |
3.1.1.3 | additional information | the recombinant enzyme displays high activity on short to medium chain length substrates, and poor activity on C18 substrates, overview, poor activity with 4-nitrophenyl palmitate, 4-nitrophenyl stearate, and 4-methylumbelliferyl oleate | Priestia megaterium | ? | - |
? | |
3.1.1.3 | additional information | the recombinant enzyme displays high activity on short to medium chain length substrates, and poor activity on C18 substrates, overview, poor activity with 4-nitrophenyl palmitate, 4-nitrophenyl stearate, and 4-methylumbelliferyl oleate | Priestia megaterium ATCC 9885 | ? | - |
? | |
3.1.1.3 | olive oil + H2O | - |
Priestia megaterium | ? | - |
? | |
3.1.1.3 | tributyrin + H2O | - |
Priestia megaterium | dibutyrin + butyrate | - |
? | |
3.1.1.3 | triolein + H2O | - |
Priestia megaterium | diolein + oleate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.1.1.3 | ? | x * 22000-22379, precursor protein, SDS-PAGE and sequence calculation, x * 19000-19542, mature protein, SDS-PAGE and sequence calculation | Priestia megaterium |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.1.3 | LipA | - |
Priestia megaterium |
3.1.1.3 | More | the cloned lipase is a member of a reduced cluster of bacterial serine-esterases grouped in subfamily I.4 of bacterial lipases, exclusive from the mesophilic or moderately thermophilic members of the genus Bacillus | Priestia megaterium |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.1.3 | 45 | - |
recombinant enzyme | Priestia megaterium |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.1.3 | 4 | 30 | pH 7.0, recombinant enzyme, 40 days, stable | Priestia megaterium |
3.1.1.3 | 50 | - |
1 h, recombinant enzyme, 9.1% remaining activity | Priestia megaterium |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.1.1.3 | 7 | - |
recombinant enzyme | Priestia megaterium |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.1.1.3 | 6 | 9 | the recombinant enzyme exhibits 87% of maximal activity at pH 6.0 and 66% at pH 9.0 | Priestia megaterium |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.1.1.3 | 4 | 12 | 1 h, room temperature, recombinant enzyme, stable | Priestia megaterium |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
3.1.1.3 | Priestia megaterium | recombinant mature protein, isoelectric focusing | - |
9.4 |
3.1.1.3 | Priestia megaterium | mature protein, sequence calculation | - |
9.42 |
3.1.1.3 | Priestia megaterium | precursor protein, sequence calculation | - |
9.56 |