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Literature summary extracted from

  • Martin, L.; Prieto, M.A.; Cortes, E.; Garcia, J.L.
    Cloning and sequencing of the pac gene encoding the penicillin G acylase of Bacillus megaterium ATCC 14945 (1995), FEMS Microbiol. Lett., 125, 287-292.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.5.1.11 synthesis production of 6-aminopenicillanate as precursor for the production of semi-synthetic beta-lactam derivatives Priestia megaterium

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.11 expressed in Escherichia coli HB101, enzyme is expressed as cytoplasmic enzyme, enzyme production not inducible by phenylacetic acid, expressed in Escherichia coli TG1, enzyme production inducible by addition of IPTG, intracellular expression Priestia megaterium

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.5.1.11 extracellular
-
 Priestia megaterium
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.11 penicillin + H2O Priestia megaterium
-
 a carboxylate + 6-aminopenicillanate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.11 Priestia megaterium Q60136 penicillin G acylase precursor; strain ATCC 14945
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.11 penicillin + H2O
-
 Priestia megaterium a carboxylate + 6-aminopenicillanate
-
 ?
3.5.1.11 penicillin G + H2O
-
 Priestia megaterium phenylacetic acid + 6-aminopenicillanate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.1.11 penicillin G acylase
-
 Priestia megaterium
3.5.1.11 PGA
-
 Priestia megaterium