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Literature summary extracted from

  • Zhang, S.; Sakuradani, E.; Ito, K.; Shimizu, S.
    Identification of a novel bifunctional DELTA12/DELTA15 fatty acid desaturase from a basidiomycete, Coprinus cinereus TD#822-2 (2007), FEBS Lett., 581, 315-319.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.19.6 expressed in Saccharomyces cerevisiae EH1315, 7.9% oleic acid and 29% linoleic acid in yeast expressing DELTA12 desaturase compared with 37% oleic acid and no detectable linoleic acid in control yeast, fatty acid composition analyzed by gas-liquid chromatography Coprinopsis cinerea
1.14.19.6 gene Cop-odeA, DNA and amino acid sequence determination and analysis, functional expression in Saccharomyces cerevisiae strain EH1315 Coprinopsis cinerea
1.14.19.39 gene Cop-odeA, DNA and amino acid sequence determination and analysis, expression in Saccharomyces cerevisiae EH1315, fatty acid compositions of the yeast transformants compared to the wild-type cells, overview Coprinopsis cinerea

Protein Variants

EC Number Protein Variants Comment Organism
1.14.19.6 additional information a recombinant Saccharomyces cerevisiae strain EH1315 expressing gene Cop-odeA accumulates four additional fatty acids identified as 9,12-hexadecadienoic acid, 9,12,15-hexadecatrienoic acid, linoleic acid, and alinolenic acid, which comprised 8.8%, 1.0%, 29.0%, and 0.6% of the total fatty acids, respectively, overview Coprinopsis cinerea

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.19.39 membrane an integral membrane-bound protein Coprinopsis cinerea 16020
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.19.6 oleic acid + ? Coprinopsis cinerea
-
 linoleic acid + ?
-
 ?
1.14.19.6 oleic acid + ? Coprinopsis cinerea TD#822-2
-
 linoleic acid + ?
-
 ?
1.14.19.6 oleic acid + ? Coprinopsis cinerea TD822-2
-
 linoleic acid + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.19.6 Coprinopsis cinerea A2A1C4 TD822-2
-
1.14.19.6 Coprinopsis cinerea A2A1C4 gene Cop-odeA
-
1.14.19.6 Coprinopsis cinerea TD#822-2 A2A1C4 gene Cop-odeA
-
1.14.19.6 Coprinopsis cinerea TD822-2 A2A1C4 TD822-2
-
1.14.19.39 Coprinopsis cinerea A2A1C4 gene Cop-odeA, isolated from soil
-
1.14.19.39 Coprinopsis cinerea TD#822-2 A2A1C4 gene Cop-odeA, isolated from soil
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.19.6 additional information the enzyme is a bifunctional fatty acid desaturase with both high DELTA12 desaturase activity and unusual DELTA15 desaturase activities Coprinopsis cinerea ?
-
 ?
1.14.19.6 additional information the enzyme is a bifunctional fatty acid desaturase with both high DELTA12 desaturase activity and unusual DELTA15 desaturase activities Coprinopsis cinerea TD#822-2 ?
-
 ?
1.14.19.6 additional information the enzyme is a bifunctional fatty acid desaturase with both high DELTA12 desaturase activity and unusual DELTA15 desaturase activities Coprinopsis cinerea TD822-2 ?
-
 ?
1.14.19.6 oleic acid + ?
-
 Coprinopsis cinerea linoleic acid + ?
-
 ?
1.14.19.6 oleic acid + ?
-
 Coprinopsis cinerea TD#822-2 linoleic acid + ?
-
 ?
1.14.19.6 oleic acid + ?
-
 Coprinopsis cinerea TD822-2 linoleic acid + ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.19.6 DELTA12DS bifunctional enzyme with strong DELTA12 and low DELTA15 desaturase activities Coprinopsis cinerea
1.14.19.39 bifunctional D12/D15 fatty acid desaturase
-
 Coprinopsis cinerea
1.14.19.39 DELTA12 fatty acid desaturase
-
 Coprinopsis cinerea
1.14.19.39 DELTA12DS
-
 Coprinopsis cinerea