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Literature summary extracted from

  • Benoit, I.; Asther, M.; Sulzenbacher, G.; Record, E.; Marmuse, L.; Parsiegla, G.; Gimbert, I.; Asther, M.; Bignon, C.
    Respective importance of protein folding and glycosylation in the thermal stability of recombinant feruloyl esterase A (2006), FEBS Lett., 580, 5815-5821.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.73 expression in Escherichia coli Aspergillus niger

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.1.73 hanging drop vapor diffusion method, FAEA produced in Aspergillus niger (AnN) and Escherichia coli (EcR) Aspergillus niger

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.73 diethyl-2-(4-hydroxy-3-methoxyphenyl)ethenephosphonate
-
 Aspergillus niger
3.1.1.73 methyl 5-N-arabinoferulamide
-
 Aspergillus niger

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.73 0.1
-
 methyl ferulate FAEA produced in Escherichia coli (EcR) Aspergillus niger
3.1.1.73 0.15
-
 methyl sinapate FAEA produced in Escherichia coli (EcR) Aspergillus niger
3.1.1.73 0.31
-
 methyl ferulate FAEA produced in Aspergillus niger Aspergillus niger
3.1.1.73 0.37
-
 methyl sinapate FAEA produced in Aspergillus niger Aspergillus niger

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.73 28550
-
 FAEA produced in Escherichia coli, mass spectrometry Aspergillus niger
3.1.1.73 30250
-
 FAEA produced in Aspergillus niger, mass spectrometry Aspergillus niger

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.73 Aspergillus niger O42807
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.73
-
 Aspergillus niger

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.73 2-chloro 4-nitrophenyl 5-O-feruloyl-alpha-sc l-arabinofuranoside + H2O
-
 Aspergillus niger ?
-
 ?
3.1.1.73 methyl ferulate + H2O
-
 Aspergillus niger ferulic acid + methanol
-
 ?
3.1.1.73 methyl sinapate + H2O
-
 Aspergillus niger sinapic acid + methanol
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.73 FAEA
-
 Aspergillus niger
3.1.1.73 feruloyl esterase A
-
 Aspergillus niger

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.73 50
-
 substrate: methyl ferulate, FAEA produced in Escherichia coli (EcR) Aspergillus niger
3.1.1.73 55
-
 substrate: methyl ferulate, FAEA produced in Aspergillus niger (AnN) Aspergillus niger

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.73 55
-
 30 min, FAEA produced in Aspergillus niger loses 15% of its activity, FAEA produced in Escherichia coli loses 94% of its activity Aspergillus niger

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.73 5
-
 substrate: methyl ferulate, FAEA produced in Aspergillus niger (AnN) and Escherichia coli (EcR) Aspergillus niger

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.1.1.73 1.4
-
 methyl 5-N-arabinoferulamide substrate: 2-chloro 4-nitrophenyl 5-O-feruloyl-alpha-L-arabinofuranoside, FAEA produced in Escherichia coli (EcR) Aspergillus niger
3.1.1.73 1.45
-
 diethyl-2-(4-hydroxy-3-methoxyphenyl)ethenephosphonate substrate: 2-chloro 4-nitrophenyl 5-O-feruloyl-alpha-L-arabinofuranoside, FAEA produced in Aspergillus niger Aspergillus niger
3.1.1.73 2.5
-
 diethyl-2-(4-hydroxy-3-methoxyphenyl)ethenephosphonate substrate: 2-chloro 4-nitrophenyl 5-O-feruloyl-alpha-L-arabinofuranoside, FAEA produced in Aspergillus niger Aspergillus niger
3.1.1.73 2.9
-
 methyl 5-N-arabinoferulamide substrate: 2-chloro 4-nitrophenyl 5-O-feruloyl-alpha-L-arabinofuranoside, FAEA produced in Escherichia coli (EcR) Aspergillus niger

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.1.1.73 Aspergillus niger calculation from sequence and verification by isoelectric focusing, FAEA produced in Aspergillus niger (AnN)
-
 4.2