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Literature summary extracted from

  • Sato, D.; Yamagata, W.; Harada, S.; Nozaki, T.
    Kinetic characterization of methionine gamma-lyases from the enteric protozoan parasite Entamoeba histolytica against physiological substrates and trifluoromethionine, a promising lead compound against amoebiasis (2008), FEBS J., 275, 548-560.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
4.4.1.11 C110G decrease in kcat values for both L-methionine and L-cysteine Entamoeba histolytica
4.4.1.11 C110S slightly reduced catalytic activites Entamoeba histolytica
4.4.1.11 C113G 20% increase in activity with L-methionine Entamoeba histolytica
4.4.1.11 C113S reduced catalytic activites Entamoeba histolytica
4.4.1.11 R55A decrease in catalytic activity Entamoeba histolytica
4.4.1.11 R58A complete loss of activity Entamoeba histolytica
4.4.1.11 Y108F almost 100% reduction in alpha-gamma-elimination of both L-methionine and homocysteine Entamoeba histolytica
4.4.1.11 Y111F about 80% reduction in alpha-gamma-elimination of both L-methionine and homocysteine Entamoeba histolytica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.4.1.11 0.1
-
 trifluoromethionine wild-type ehMGL1, pH 7.0, 37°C Entamoeba histolytica
4.4.1.11 0.19
-
 L-methionine mutant C110G, pH 7.0, 37°C Entamoeba histolytica
4.4.1.11 0.29
-
 trifluoromethionine mutant Y111F, pH 7.0, 37°C Entamoeba histolytica
4.4.1.11 0.34
-
 L-cysteine mutant C110G, pH 7.0, 37°C Entamoeba histolytica
4.4.1.11 0.46
-
 L-cysteine mutant C110S, pH 7.0, 37°C Entamoeba histolytica
4.4.1.11 0.57
-
 trifluoromethionine mutant Y108F, pH 7.0, 37°C Entamoeba histolytica
4.4.1.11 0.61
-
 L-methionine wild-type ehMGL1, pH 7.0, 37°C Entamoeba histolytica
4.4.1.11 0.64
-
 L-cysteine wild-type ehMGL1, pH 7.0, 37°C Entamoeba histolytica
4.4.1.11 0.72
-
 L-methionine mutant C110S, pH 7.0, 37°C Entamoeba histolytica
4.4.1.11 0.83
-
 trifluoromethionine mutant R55A, pH 7.0, 37°C Entamoeba histolytica
4.4.1.11 0.92
-
 trifluoromethionine wild-type ehMGL2, pH 7.0, 37°C Entamoeba histolytica
4.4.1.11 1.01
-
 L-cysteine mutant Y108F, pH 7.0, 37°C Entamoeba histolytica
4.4.1.11 1.47
-
 DL-homocysteine wild-type ehMGL2, pH 7.0, 37°C Entamoeba histolytica
4.4.1.11 1.62
-
 trifluoromethionine mutant R58A, pH 7.0, 37°C Entamoeba histolytica
4.4.1.11 1.7
-
 L-cysteine wild-type ehMGL2, pH 7.0, 37°C Entamoeba histolytica
4.4.1.11 3.03
-
 DL-homocysteine wild-type ehMGL1, pH 7.0, 37°C Entamoeba histolytica
4.4.1.11 3.58
-
 L-methionine wild-type ehMGL2, pH 7.0, 37°C Entamoeba histolytica
4.4.1.11 5.45
-
 trifluoromethionine mutant C113S, pH 7.0, 37°C Entamoeba histolytica
4.4.1.11 6.28
-
 O-acetyl-L-serine wild-type ehMGL1, pH 7.0, 37°C Entamoeba histolytica
4.4.1.11 15.12
-
 L-methionine mutant C113S, pH 7.0, 37°C Entamoeba histolytica
4.4.1.11 52.33
-
 O-acetyl-L-serine wild-type ehMGL2, pH 7.0, 37°C Entamoeba histolytica

Organism

EC Number Organism UniProt Comment Textmining
4.4.1.11 Entamoeba histolytica Q86D27 isoform ehMGL2
-
4.4.1.11 Entamoeba histolytica Q86D28 isoform ehMGL1
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.4.1.11 DL-homocysteine + H2O
-
 Entamoeba histolytica hydrogen sulfide + NH3 + 2-oxobutanoate
-
 ?
4.4.1.11 L-cysteine + H2O
-
 Entamoeba histolytica sulfide + NH3 + pyruvate
-
 ?
4.4.1.11 L-methionine + H2O
-
 Entamoeba histolytica methanethiol + NH3 + 2-oxobutanoate
-
 ?
4.4.1.11 additional information under normal conditions, isoform ehMGL1 is involved in degradation of L-methionine Entamoeba histolytica ?
-
 ?
4.4.1.11 additional information under normal conditions, isoform ehMGL2 is not involved degradation of L-methionine Entamoeba histolytica ?
-
 ?
4.4.1.11 O-acetyl-L-serine + H2O
-
 Entamoeba histolytica 2-oxopropanoate + NH3 + acetate
-
 ?
4.4.1.11 trifluoromethionine + H2O
-
 Entamoeba histolytica trifluoromethanethiol + NH3 + 2-oxobutanoate
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.4.1.11 0.057 0.65 DL-homocysteine wild-type ehMGL2, pH 7.0, 37°C Entamoeba histolytica
4.4.1.11 0.24
-
 trifluoromethionine mutant C113S, pH 7.0, 37°C Entamoeba histolytica
4.4.1.11 0.36
-
 L-methionine mutant C110G, pH 7.0, 37°C Entamoeba histolytica
4.4.1.11 0.47
-
 L-methionine mutant C113S, pH 7.0, 37°C Entamoeba histolytica
4.4.1.11 0.67
-
 L-cysteine mutant Y108F, pH 7.0, 37°C Entamoeba histolytica
4.4.1.11 0.78
-
 L-cysteine mutant C110S, pH 7.0, 37°C Entamoeba histolytica
4.4.1.11 0.8
-
 L-cysteine wild-type ehMGL2, pH 7.0, 37°C Entamoeba histolytica
4.4.1.11 0.81
-
 trifluoromethionine wild-type ehMGL1, pH 7.0, 37°C Entamoeba histolytica
4.4.1.11 0.93
-
 L-methionine mutant C110S, pH 7.0, 37°C Entamoeba histolytica
4.4.1.11 0.97
-
 trifluoromethionine wild-type ehMGL2, pH 7.0, 37°C Entamoeba histolytica
4.4.1.11 1.01
-
 L-cysteine mutant C110G, pH 7.0, 37°C Entamoeba histolytica
4.4.1.11 1.11
-
 L-methionine wild-type ehMGL2, pH 7.0, 37°C Entamoeba histolytica
4.4.1.11 1.19
-
 trifluoromethionine mutant R58A, pH 7.0, 37°C Entamoeba histolytica
4.4.1.11 1.26
-
 trifluoromethionine mutant R55A, pH 7.0, 37°C Entamoeba histolytica
4.4.1.11 1.59
-
 L-cysteine wild-type ehMGL1, pH 7.0, 37°C Entamoeba histolytica
4.4.1.11 1.74
-
 O-acetyl-L-serine wild-type ehMGL1, pH 7.0, 37°C Entamoeba histolytica
4.4.1.11 1.82
-
 L-methionine wild-type ehMGL1, pH 7.0, 37°C Entamoeba histolytica
4.4.1.11 2.22
-
 trifluoromethionine mutant Y108F, pH 7.0, 37°C Entamoeba histolytica
4.4.1.11 3.13
-
 DL-homocysteine wild-type ehMGL1, pH 7.0, 37°C Entamoeba histolytica
4.4.1.11 3.91
-
 DL-homocysteine wild-type ehMGL1, pH 7.0, 37°C Entamoeba histolytica
4.4.1.11 5.8
-
 trifluoromethionine mutant Y111F, pH 7.0, 37°C Entamoeba histolytica
4.4.1.11 6.22
-
 O-acetyl-L-serine wild-type ehMGL2, pH 7.0, 37°C Entamoeba histolytica
4.4.1.11 10.56
-
 DL-homocysteine wild-type ehMGL2, pH 7.0, 37°C Entamoeba histolytica
4.4.1.11 17.46
-
 trifluoromethionine wild-type ehMGL2, pH 7.0, 37°C Entamoeba histolytica