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Literature summary extracted from

  • Morana, A.; Paris, O.; Maurelli, L.; Rossi, M.; Cannio, R.
    Gene cloning and expression in Escherichia coli of a bi-functional beta-D-xylosidase/alpha-L-arabinosidase from Sulfolobus solfataricus involved in xylan degradation (2007), Extremophiles, 11, 123-132.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.37 expression in Escherichia coli Saccharolobus solfataricus
3.2.1.55 expression in Escherichia coli Saccharolobus solfataricus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.37 82000
-
 4 * 82000, SDS-PAGE Saccharolobus solfataricus
3.2.1.37 338800
-
 gel filtration Saccharolobus solfataricus
3.2.1.55 82000
-
 4 * 82000, SDS-PAGE Saccharolobus solfataricus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.55 additional information Saccharolobus solfataricus specifically induced by xylan and repressed by monosaccharides like D-glucose and L-arabinose ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.37 Saccharolobus solfataricus
-
 bi-functional beta-D-xylosidase/alpha-L-arabinosidase
-
3.2.1.55 Saccharolobus solfataricus
-
-
-
3.2.1.55 Saccharolobus solfataricus P2
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.37 recombinant protein Saccharolobus solfataricus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.37 3.63
-
 pH 6.5, 75°C Saccharolobus solfataricus
3.2.1.55 3.63
-
-
 Saccharolobus solfataricus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.37 2-nitrophenyl beta-D-glucopyranoside + H2O 1.7% of the activity with 4-nitrophenyl-alpha-L-arabinofuranoside Saccharolobus solfataricus 2-nitrophenol + beta-D-glucose
-
 ?
3.2.1.37 2-nitrophenyl beta-D-glucopyranoside + H2O 1.7% of the activity with 4-nitrophenyl-alpha-L-arabinofuranoside Saccharolobus solfataricus P2 2-nitrophenol + beta-D-glucose
-
 ?
3.2.1.37 2-nitrophenyl beta-D-xylopyranoside + H2O 3% of the activity with 4-nitrophenyl-alpha-L-arabinofuranoside Saccharolobus solfataricus 2-nitrophenol + D-xylose
-
 ?
3.2.1.37 2-nitrophenyl beta-D-xylopyranoside + H2O 3% of the activity with 4-nitrophenyl-alpha-L-arabinofuranoside Saccharolobus solfataricus P2 2-nitrophenol + D-xylose
-
 ?
3.2.1.37 4-nitrophenyl alpha-L-arabinofuranoside + H2O
-
 Saccharolobus solfataricus 4-nitrophenol + alpha-L-arabinofuranose
-
 ?
3.2.1.37 4-nitrophenyl alpha-L-arabinofuranoside + H2O
-
 Saccharolobus solfataricus P2 4-nitrophenol + alpha-L-arabinofuranose
-
 ?
3.2.1.37 4-nitrophenyl beta-D-glucopyranoside + H2O 9% of the activity with 4-nitrophenyl-alpha-L-arabinofuranoside Saccharolobus solfataricus 4-nitrophenol + beta-D-glucose
-
 ?
3.2.1.37 4-nitrophenyl beta-D-glucopyranoside + H2O 9% of the activity with 4-nitrophenyl-alpha-L-arabinofuranoside Saccharolobus solfataricus P2 4-nitrophenol + beta-D-glucose
-
 ?
3.2.1.37 4-nitrophenyl beta-D-xylopyranoside + H2O 14% of the activity with 4-nitrophenyl-alpha-L-arabinofuranoside Saccharolobus solfataricus 4-nitrophenol + beta-D-xylose
-
 ?
3.2.1.37 4-nitrophenyl beta-D-xylopyranoside + H2O 14% of the activity with 4-nitrophenyl-alpha-L-arabinofuranoside Saccharolobus solfataricus P2 4-nitrophenol + beta-D-xylose
-
 ?
3.2.1.37 4-nitrophenyl-alpha-L-arabinopyranoside + H2O 46% of the activity with 4-nitrophenyl-alpha-L-arabinofuranoside Saccharolobus solfataricus 4-nitrophenol + alpha-L-arabinose
-
 ?
3.2.1.55 2-nitrophenyl beta-D-glucopyranoside + H2O 1.7% of activity with p-nitrophenyl-alpha-L-arabinofuranoside Saccharolobus solfataricus 2-nitrophenol + D-glucopyranose
-
 ?
3.2.1.55 4-nitrophenyl beta-D-glucopyranoside + H2O 9% of activity with p-nitrophenyl-alpha-L-arabinofuranoside Saccharolobus solfataricus 4-nitrophenol + D-glucopyranose
-
 ?
3.2.1.55 additional information specifically induced by xylan and repressed by monosaccharides like D-glucose and L-arabinose Saccharolobus solfataricus ?
-
 ?
3.2.1.55 o-nitrophenyl-beta-D-xylopyranoside + H2O 3% of activity with p-nitrophenyl-alpha-L-arabinofuranoside Saccharolobus solfataricus o-nitrophenol + beta-D-xylopyranose
-
 ?
3.2.1.55 p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
 Saccharolobus solfataricus p-nitrophenol + L-arabinofuranose
-
 ?
3.2.1.55 p-nitrophenyl-alpha-L-arabinopyranoside + H2O 46% of activity with p-nitrophenyl-alpha-L-arabinofuranoside Saccharolobus solfataricus p-nitrophenol + alpha-L-arabinopyranose
-
 ?
3.2.1.55 p-nitrophenyl-beta-D-xylopyranoside + H2O 14% of activity with p-nitrophenyl-alpha-L-arabinofuranoside Saccharolobus solfataricus p-nitrophenol + beta-D-xylopyranose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.37 tetramer 4 * 82000, SDS-PAGE Saccharolobus solfataricus
3.2.1.55 tetramer 4 * 82000, SDS-PAGE Saccharolobus solfataricus

Synonyms

EC Number Synonyms Comment Organism
3.2.1.55 beta-D-xylosidase/alpha-L-arabinosidase
-
 Saccharolobus solfataricus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.37 80 85 beta-D-xylosidase activity Saccharolobus solfataricus
3.2.1.55 80
-
-
 Saccharolobus solfataricus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.37 80
-
 24 h, stable Saccharolobus solfataricus
3.2.1.37 90
-
 half-life 10.5 h Saccharolobus solfataricus
3.2.1.55 80
-
 24 h, stable Saccharolobus solfataricus
3.2.1.55 90
-
 half-life: 2 h Saccharolobus solfataricus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.37 6.5
-
 both beta-D-xylosidase and alpha-L-arabinosidase activity Saccharolobus solfataricus
3.2.1.55 6.5
-
-
 Saccharolobus solfataricus