EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.1.21.4 | Co2+ | complete inhibition at 1 mM | Priestia megaterium | |
3.1.21.4 | Mn2+ | complete inhibition at 3 mM | Priestia megaterium | |
3.1.21.4 | NaCl | inhibitory above 0.2 mM | Priestia megaterium | |
3.1.21.4 | Ni2+ | complete inhibition at 1 mM | Priestia megaterium | |
3.1.21.4 | Zn2+ | - |
Priestia megaterium |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.1.21.4 | Co2+ | can replace Mn2+ but yields a much lower activity than Mg2+, highest activity at 0.1 mM, inhibitory at high concentrations | Priestia megaterium | |
3.1.21.4 | Mg2+ | highest activity at 5-7 mM | Priestia megaterium | |
3.1.21.4 | Mn2+ | can replace Mn2+ but yields a much lower activity than Mg2+, highest activity at 0.3 mM, inhibitory at high concentrations | Priestia megaterium | |
3.1.21.4 | Ni2+ | can replace Mn2+ but yields a much lower activity than Mg2+, highest activity at 0.1 mM, inhibitory at high concentrations | Priestia megaterium |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.1.21.4 | 30000 | - |
x * 30000, SDS-PAGE, native mass by gel filtration | Priestia megaterium |
3.1.21.4 | 60000 | - |
gel filtration | Priestia megaterium |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.21.4 | dsDNA + H2O | Priestia megaterium | recognition sequence is 5-GGACC-3/3-CCTGG-5, enzyme cleaves between the guanosin residues at both strands | ? | sticky end fragments | ? | |
3.1.21.4 | dsDNA + H2O | Priestia megaterium 216 | recognition sequence is 5-GGACC-3/3-CCTGG-5, enzyme cleaves between the guanosin residues at both strands | ? | sticky end fragments | ? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.21.4 | Priestia megaterium | - |
- |
- |
3.1.21.4 | Priestia megaterium 216 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.21.4 | - |
Priestia megaterium |
EC Number | Storage Stability | Organism |
---|---|---|
3.1.21.4 | -20°C, 10 mM phosphate buffer, pH 7.4, 0.1 mM EDTA, 2 mM dithiothreitol, 0.2 M NaCl, 50% glycerol, fully stable for at least 1 year | Priestia megaterium |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.21.4 | dsDNA + H2O | recognition sequence is 5-GGACC-3/3-CCTGG-5, enzyme cleaves between the guanosin residues at both strands | Priestia megaterium | ? | sticky end fragments | ? | |
3.1.21.4 | dsDNA + H2O | recognition sequence is 5-GGACC-3/3-CCTGG-5, enzyme cleaves between the guanosin residues at both strands | Priestia megaterium 216 | ? | sticky end fragments | ? | |
3.1.21.4 | pBR322 DNA + H2O | - |
Priestia megaterium | ? | - |
? | |
3.1.21.4 | pBR322 DNA + H2O | - |
Priestia megaterium 216 | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.1.21.4 | dimer | x * 30000, SDS-PAGE, native mass by gel filtration | Priestia megaterium |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.21.4 | site-specific endonuclease Bme216I | recognition site is identical with the endonuclease AvaII from Anabaena variabilis | Priestia megaterium |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.1.21.4 | 6 | 9 | - |
Priestia megaterium |