EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.4.22.B6 | DTT | no activity in buffer lacking DTT | Trypanosoma congolense |
EC Number | Application | Comment | Organism |
---|---|---|---|
3.4.22.B6 | medicine | TcoCBc1 elicits an important immune response in experimentally infected cattle. The protein is a potential therapeutic target and is a plausible antigen for Trypanosoma congolense diagnosis | Trypanosoma congolense |
EC Number | Cloned (Comment) | Organism |
---|---|---|
3.4.22.B6 | six-His-tagged protein is expressed in Pichia pastoris strain X-33 | Trypanosoma congolense |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.4.22.B6 | acetyl-Leu-Val-lysinal | 350 nM, 37°C, pH 4.5, 1 h, 15% inhibition | Trypanosoma congolense | |
3.4.22.B6 | CA074 | 350 nM, 37°C, pH 4.5, 1 h, complete inhibition | Trypanosoma congolense | |
3.4.22.B6 | CBZ-Phe-Gly-NHO-Bz-pMe | 350 nM, 37°X, pH 4.5, 1 h, 87% inhibition | Trypanosoma congolense | |
3.4.22.B6 | cystatin | 350 nM, 37°C, pH 4.5, 1 h, 23% inhibition | Trypanosoma congolense | |
3.4.22.B6 | E-64 | 350 nM, 37°C, pH 4.5, 1 h, complete inhibition | Trypanosoma congolense |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.22.B6 | 0.1234 | - |
Nalpha-benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin | - |
Trypanosoma congolense | |
3.4.22.B6 | 0.1733 | - |
N2-benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin | - |
Trypanosoma congolense |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.4.22.B6 | lysosome | - |
Trypanosoma congolense | 5764 | - |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.4.22.B6 | additional information | - |
SDS-PAGE analysis of the eluted recombinant protein reveals a prominent band of 39000 Da and a minor protein of 40000 Da. These bands correspond to glycosylated forms of a 35000 Da protein, the expected size of the protease | Trypanosoma congolense |
3.4.22.B6 | additional information | - |
SDS-PAGE analysis of the eluted recombinant protein reveals a prominent band of 40000 Da and a minor protein of 50000 Da. These bands correspond to glycosylated forms of a 36000 Da protein, the expected size of the protease | Trypanosoma congolense |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.4.22.B6 | Trypanosoma congolense | - |
- |
- |
3.4.22.B6 | Trypanosoma congolense | B2C323 | - |
- |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
3.4.22.B6 | proteolytic modification | TcoCBc1 is able to autocatalyze its maturation 21 residues upstream of the predicted start of the catalytic domain | Trypanosoma congolense |
3.4.22.B6 | proteolytic modification | TcoCBc6 is able to autocatalyze its maturation 31 residues, upstream of the predicted start of the catalytic domain | Trypanosoma congolense |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.4.22.B6 | - |
Trypanosoma congolense |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.4.22.B6 | bloodstream form | - |
Trypanosoma congolense | - |
3.4.22.B6 | bloodstream form | expressed mainly in bloodstream form | Trypanosoma congolense | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.22.B6 | 2-aminobenzoyl-Phe-Arg-Ala-(2,4-dinitrophenyl)-epsilon-NH2-lysine + H2O | - |
Trypanosoma congolense | ? | - |
? | |
3.4.22.B6 | benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin + H2O | - |
Trypanosoma congolense | CBZ-Arg-Arg + 7-amino-4-methylcoumarin | - |
? | |
3.4.22.B6 | benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin + H2O | - |
Trypanosoma congolense | benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin | - |
? | |
3.4.22.B6 | additional information | no hydrolysis of benzoyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin and benzoyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin | Trypanosoma congolense | ? | - |
? | |
3.4.22.B6 | Nalpha-benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin + H2O | - |
Trypanosoma congolense | Nalpha-benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.4.22.B6 | CBc1 | - |
Trypanosoma congolense |
3.4.22.B6 | CBc6 | - |
Trypanosoma congolense |
3.4.22.B6 | TcoCBc1 | - |
Trypanosoma congolense |
3.4.22.B6 | TcoCBc6 | - |
Trypanosoma congolense |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.22.B6 | 1.09 | - |
Nalpha-benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin | - |
Trypanosoma congolense | |
3.4.22.B6 | 3 | 6 | CBZ-Arg-Arg-7-amido-4-methylcoumarin | - |
Trypanosoma congolense | |
3.4.22.B6 | 3.49 | - |
N2-benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin | - |
Trypanosoma congolense |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.4.22.B6 | 4.5 | - |
- |
Trypanosoma congolense |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.4.22.B6 | 4 | 6.5 | pH 4.0: about 30% of maximal activity, pH 6.5: about 75% of maximal activity | Trypanosoma congolense |