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Literature summary extracted from

  • Westfall, P.J.; Thorner, J.
    Analysis of mitogen-activated protein kinase signaling specificity in response to hyperosmotic stress: use of an analog-sensitive HOG1 allele (2006), Eukaryot. Cell, 5, 1215-1228.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.11.24 additional information upon hyperosmotic stress, Hog1 is activated by phosphorylation Saccharomyces cerevisiae
2.7.11.24 sorbitol upon exposure to 1000 mM sorbitol, Hog1 is phosphorylated rapidly, with modification peaking at 10 to 20 min and then declining as cells adapt Saccharomyces cerevisiae

Application

EC Number Application Comment Organism
2.7.11.24 additional information a role for active Hog1 in maintaining signaling specificity under conditions of persistently high external osmolarity Saccharomyces cerevisiae

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.11.24 into vector pCR2.1, expressed in Escherichia coli DH5alpha Saccharomyces cerevisiae

Protein Variants

EC Number Protein Variants Comment Organism
2.7.11.24 D144A kinase-dead mutant, is unable to grow on 1000 mM sorbitol Saccharomyces cerevisiae
2.7.11.24 K52R retains residual activity that permitts slow but readily detectable growth on 1000 mM sorbitol, residual growth is eliminated in the presence of 1-NM-PP1 Saccharomyces cerevisiae
2.7.11.24 additional information hog1delta mutant, is unable to grow in the presence of 1-NM-PP1 Saccharomyces cerevisiae
2.7.11.24 T100A Hog1-as-mutant, fully functional analog-sensitive allele of HOG1, is unable to grow in the presence of 1-NM-PP1, permits acute inhibition of the enzyme without other detectable perturbations of the cell, in the presence of inhibitor Rck2 modification is completely abrogated Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.11.24 additional information wild-type Hog1 phosphorylation is unaffected by 1-NM-PP1 Saccharomyces cerevisiae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.11.24 cytosol during growth under isosmotic conditions, presence of Hog1, the Hog1-as–mutant, and mutants K52R and D144A Saccharomyces cerevisiae 5829
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2.7.11.24 nucleus within 10 min after exposure to 1000 mM sorbitol, Hog1 and Hog1-as–mutant almost exclusively present in the nucleus, nuclear import of activated Hog1 requires its catalytic function Saccharomyces cerevisiae 5634
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Organism

EC Number Organism UniProt Comment Textmining
2.7.11.24 Saccharomyces cerevisiae
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.24 ATP + Rck2
-
 Saccharomyces cerevisiae ADP + phosphorylated Rck2
-
 ?
2.7.11.24 additional information kinase activity of Hog1 is required to promote its own dephosphorylation after hyperosmotic-stress-induced activation, moreover, catalytic activity of Hog1 is required continuously to prevent cross talk between the the high-osmolarity glycerol pathway and both the pheromone response and invasive growth pathways Saccharomyces cerevisiae ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.11.24 Hog1
-
 Saccharomyces cerevisiae
2.7.11.24 MAPK
-
 Saccharomyces cerevisiae
2.7.11.24 mitogen-activated protein kinase
-
 Saccharomyces cerevisiae