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Literature summary extracted from

  • Voutilainen, S.P.; Boer, H.; Linder, M.B.; Puranen, T.; Rouvinen, J.; Vehmaanperae, J.; Koivula, A.
    Heterologous expression of Melanocarpus albomyces cellobiohydrolase Cel7B, and random mutagenesis to improve its thermostability (2007), Enzyme Microb. Technol., 41, 234-243.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.176 expressed in Saccharomyces cerevisiae strain NY179 Melanocarpus albomyces

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.176 A163T improved thermostability Melanocarpus albomyces
3.2.1.176 A30T mutant shows improved unfolding temperature by 1.5°C compared to the wild type enzyme (64.5°C) Melanocarpus albomyces
3.2.1.176 A3D improved thermostability Melanocarpus albomyces
3.2.1.176 D411G improved thermostability Melanocarpus albomyces
3.2.1.176 G184D mutant has higher specific activity and shows wild type unfolding temperature (64.5°C) Melanocarpus albomyces
3.2.1.176 G346D improved thermostability Melanocarpus albomyces
3.2.1.176 G386V improved thermostability Melanocarpus albomyces
3.2.1.176 G75D improved thermostability Melanocarpus albomyces
3.2.1.176 P114T improved thermostability Melanocarpus albomyces
3.2.1.176 R166Q improved thermostability Melanocarpus albomyces
3.2.1.176 S290T mutant has slightly lower specific activity and shows improved unfolding temperature by 3.5°C compared to the wild type enzyme (64.5°C) Melanocarpus albomyces
3.2.1.176 T56S improved thermostability Melanocarpus albomyces
3.2.1.176 Y100N improved thermostability Melanocarpus albomyces

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.176 47500
-
 SDS-PAGE Melanocarpus albomyces

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.176 Melanocarpus albomyces
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.176 DEAE Sepharose FF column chromatography Melanocarpus albomyces

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.176 4-methylumbelliferyl beta-lactoside + H2O
-
 Melanocarpus albomyces 4-methylumbelliferone + lactose
-
 ?
3.2.1.176 avicel + H2O
-
 Melanocarpus albomyces cellobiose + ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.176 Cel7B
-
 Melanocarpus albomyces
3.2.1.176 cellobiohydrolase
-
 Melanocarpus albomyces

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.176 55
-
-
 Melanocarpus albomyces

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.176 Melanocarpus albomyces pI around 4, isoelectric focusing
-
 4