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Literature summary extracted from
- Production of thermostable beta-mannosidase by a strain of Thermoascus aurantiacus: Isolation, partial purification and characterization of the enzyme (2007), Enzyme Microb. Technol., 40, 969-975.
No PubMed abstract available
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.25 | 1.1 | - |
p-nitrophenyl-beta-D-mannoside | in sodium citrate buffer (100 mM, pH 3) | Thermoascus aurantiacus |  |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.25 | 99900 | - |
SDS-PAGE | Thermoascus aurantiacus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.25 | Thermoascus aurantiacus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.25 | Mono S HR 5/5 column chromatography and Superdex 200 gel filtration | Thermoascus aurantiacus |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.25 | 0.3 | - |
culture filtrate | Thermoascus aurantiacus |
| 3.2.1.25 | 3.66 | - |
after 11.4fold puification | Thermoascus aurantiacus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.25 | 4-nitrophenyl beta-D-mannopyranoside + methyl alpha-D-mannopyranoside | the enzyme shows low transglycosylation activity at 50°C and pH 5.0 | Thermoascus aurantiacus | 4-nitrophenyl alpha-D-mannopyranoside + methyl beta-D-mannopyranoside | - |
? | |
| 3.2.1.25 | p-nitrophenyl-beta-D-mannoside + H2O | - |
Thermoascus aurantiacus | p-nitrophenol + alpha-D-mannose | - |
? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.25 | 76 | - |
- |
Thermoascus aurantiacus |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.25 | 76 | - |
half-life of 10 min at 76°C | Thermoascus aurantiacus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.25 | 2.5 | 3 | - |
Thermoascus aurantiacus |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.25 | 5.9 | - |
exhibits highest pH stability at pH 5.9 after 5 h incubation at 50°C | Thermoascus aurantiacus |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 3.2.1.25 | Thermoascus aurantiacus | isoelectric focusing | - |
4.8 |
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Release 2023.1 (January 2023)
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