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Literature summary extracted from
- Purification and characterization of a novel ginsenoside-hydrolyzing beta-D-glucosidase from the China white jade snail (Achatina fulica) (2007), Enzyme Microb. Technol., 40, 1358-1366.
No PubMed abstract available
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.21 | 0.224 | - |
4-nitrophenyl-beta-D-glucopyranoside | pH 5.0, 50°C | Lissachatina fulica |  |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 110000 | - |
2 * 110000, SDS-PAGE | Lissachatina fulica |
| 3.2.1.21 | 220000 | - |
gel filtration | Lissachatina fulica |
| 3.2.1.B23 | 110000 | - |
2 * 110000, SDS-PAGE | Lissachatina fulica |
| 3.2.1.B23 | 220000 | - |
native PAGE | Lissachatina fulica |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.21 | ginsenoside Rb1 + 3 H2O | Lissachatina fulica | - |
(3beta,5xi,6alpha,12beta,13xi)-3,6,12-trihydroxydammar-24-en-20-yl beta-D-glucopyranoside + 3 beta-D-glucose | - |
? | |
| 3.2.1.21 | ginsenoside Rb2 + 2 H2O | Lissachatina fulica | - |
(3beta,5xi,6alpha,12beta,13xi)-3,6,12-trihydroxydammar-24-en-20-yl 6-O-alpha-L-arabinopyranosyl-beta-D-glucopyranoside + 2 beta-D-glucose | - |
? | |
| 3.2.1.21 | ginsenoside Rb3 + H2O | Lissachatina fulica | - |
20-O-beta -D-xylopyranosyl-1,6-beta-D-glucopyranosyl-20(S)-protopanaxadiol + beta-D-glucose | - |
? | |
| 3.2.1.21 | ginsenoside Rc + H2O | Lissachatina fulica | - |
(3beta,5xi,6alpha,12beta,13xi)-3,6,12-trihydroxydammar-24-en-20-yl 6-O-beta-D-xylopyranosyl-beta-D-glucopyranoside + beta-D-glucose | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.21 | Lissachatina fulica | - |
China white jade snail | - |
| 3.2.1.B23 | Lissachatina fulica | - |
- |
- |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 3.2.1.21 | glycoprotein | - |
Lissachatina fulica |
| 3.2.1.B23 | additional information | might be a glycoprotein | Lissachatina fulica |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.21 | native enzyme 626fold from acetone powder of viscera by two steps of anion exchange chromatography, hydrophobic interaction chromatography, and two steps of gel filtration | Lissachatina fulica |
| 3.2.1.B23 | - |
Lissachatina fulica |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.2.1.21 | viscus | - |
Lissachatina fulica | - |
| 3.2.1.B23 | viscus | - |
Lissachatina fulica | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 162.8 | - |
purified enzyme | Lissachatina fulica |
| 3.2.1.B23 | 162.8 | - |
pH 5.0, 50°C, substrate: 4-nitrophenyl-beta-D-glucopyranoside | Lissachatina fulica |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.21 | 2-nitrophenyl beta-D-glucopyranoside + H2O | best substrate | Lissachatina fulica | 2-nitrophenol + beta-D-glucose | - |
? | |
| 3.2.1.21 | 4-methylumbelliferyl-beta-D-glucopyranoside + H2O | high activity | Lissachatina fulica | 4-methylumbelliferol + beta-D-glucose | - |
? | |
| 3.2.1.21 | 4-nitrophenyl beta-D-glucopyranoside + H2O | high activity | Lissachatina fulica | 4-nitrophenol + beta-D-glucose | - |
? | |
| 3.2.1.21 | cellobiose + H2O | - |
Lissachatina fulica | 2 beta-D-glucose | - |
? | |
| 3.2.1.21 | gentiobiose + H2O | - |
Lissachatina fulica | 2 beta-D-glucose | - |
? | |
| 3.2.1.21 | ginsenoside Rb1 + 3 H2O | - |
Lissachatina fulica | (3beta,5xi,6alpha,12beta,13xi)-3,6,12-trihydroxydammar-24-en-20-yl beta-D-glucopyranoside + 3 beta-D-glucose | - |
? | |
| 3.2.1.21 | ginsenoside Rb1 + 3 H2O | - |
Lissachatina fulica | (3beta,5xi,6alpha,12beta,13xi)-3,6,12-trihydroxydammar-24-en-20-yl beta-D-glucopyranoside + 3 beta-D-glucose | i.e. compound K | ? | |
| 3.2.1.21 | ginsenoside Rb2 + 2 H2O | - |
Lissachatina fulica | (3beta,5xi,6alpha,12beta,13xi)-3,6,12-trihydroxydammar-24-en-20-yl 6-O-alpha-L-arabinopyranosyl-beta-D-glucopyranoside + 2 beta-D-glucose | - |
? | |
| 3.2.1.21 | ginsenoside Rb2 + 2 H2O | - |
Lissachatina fulica | (3beta,5xi,6alpha,12beta,13xi)-3,6,12-trihydroxydammar-24-en-20-yl 6-O-alpha-L-arabinopyranosyl-beta-D-glucopyranoside + 2 beta-D-glucose | i.e. compound Y | ? | |
| 3.2.1.21 | ginsenoside Rb3 + H2O | - |
Lissachatina fulica | 20-O-beta -D-xylopyranosyl-1,6-beta-D-glucopyranosyl-20(S)-protopanaxadiol + beta-D-glucose | - |
? | |
| 3.2.1.21 | ginsenoside Rb3 + H2O | - |
Lissachatina fulica | 20-O-beta -D-xylopyranosyl-1,6-beta-D-glucopyranosyl-20(S)-protopanaxadiol + beta-D-glucose | i.e. compound Mx | ? | |
| 3.2.1.21 | ginsenoside Rc + H2O | - |
Lissachatina fulica | (3beta,5xi,6alpha,12beta,13xi)-3,6,12-trihydroxydammar-24-en-20-yl 6-O-beta-D-xylopyranosyl-beta-D-glucopyranoside + beta-D-glucose | - |
? | |
| 3.2.1.21 | ginsenoside Rc + H2O | - |
Lissachatina fulica | (3beta,5xi,6alpha,12beta,13xi)-3,6,12-trihydroxydammar-24-en-20-yl 6-O-beta-D-xylopyranosyl-beta-D-glucopyranoside + beta-D-glucose | i.e. compound Mc | ? | |
| 3.2.1.21 | additional information | substrate specificity with various 4-nitrophenol glycosides, overview, poor activity with lactose, sucrose, trehalose, and ginsenoside Rd, the enzyme can cleave both beta-1,2-glucosidic linkage at 3-C and beta-1,6-glucosidic linkage at 20-C of ginsenosides and can hydrolyze ginsenosides Rb1, Rb2, Rb3 and Rc into their active metabolites, compound K, compound Y, Mx, and Mc, respectively | Lissachatina fulica | ? | - |
? | |
| 3.2.1.21 | sophorose + H2O | - |
Lissachatina fulica | 2 beta-D-glucose | - |
? | |
| 3.2.1.B23 | ginsenoside Rb1 + H2O | the enzyme hydrolyzes the 20-O-beta-D-(1->6)-glucoside and both glucosidic linkages at 3-C. The enzyme prefers the 20-C, beta-(1->6)-glucosidic bond to the 3-C, beta-(1->2)-glucosidic bond. Ginsenoside Rb1 is firstly converted to ginsenoside Rd, subsequently to ginsenoside F2, and finally to ginsenoside C-K after 24 h of incubation | Lissachatina fulica | ginsenoside C-K + 3 D-glucose | - |
? | |
| 3.2.1.B23 | ginsenoside Rb2 + 2 H2O | the enzyme hydrolyzes both glucosidic linkages at 3-C. Ginsenoside Rb2 is firstly converted to ginsenoside C-O and subsequently to ginsenoside C-Y | Lissachatina fulica | ginsenoside C-Y + 2 D-glucose | - |
? | |
| 3.2.1.B23 | ginsenoside Rb3 + H2O | the enzyme hydrolyzes both glucosidic linkages at 3-C | Lissachatina fulica | ginsenoside Mx + 2 D-glucose | - |
? | |
| 3.2.1.B23 | ginsenoside Rc + H2O | the enzyme hydrolyzes both glucosidic linkages at 3-C | Lissachatina fulica | ginsenoside Mc + 2 D-glucose | - |
? | |
| 3.2.1.B23 | additional information | the enzyme does not cleave the 20-C-beta-D-(6->1)-arabinopyranoside of ginsenoside Rb2, the 20-C-beta-D-(6->1)-xyloside of ginsenoside Rb3, the 20-O-beta-D-(6->1)-arabinofuranoide of ginsenoside Rc. The enzyme also hydrolyses 4-nitrophenyl-beta-D-glycosides, cellobiose, sophorose, methyl beta-glucopyranoside, hexyl beta-D-glucopyranoside, heptyl beta-D-glucopyranoside, decyl beta-D-glucopyranoside, dodecyl beta-D-glucopyranoside | Lissachatina fulica | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.2.1.21 | dimer | 2 * 110000, SDS-PAGE | Lissachatina fulica |
| 3.2.1.B23 | dimer | 2 * 110000, SDS-PAGE | Lissachatina fulica |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.1.21 | ginsenoside-hydrolyzing beta-D-glucosidase | - |
Lissachatina fulica |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 50 | - |
- |
Lissachatina fulica |
| 3.2.1.B23 | 50 | - |
assay at | Lissachatina fulica |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 50 | - |
stable for 8 h | Lissachatina fulica |
| 3.2.1.B23 | 30 | 50 | 8 h, enzyme retains full activity | Lissachatina fulica |
| 3.2.1.B23 | 60 | - |
stable for 30 min, 72% loss of activity after 30 min | Lissachatina fulica |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 5 | - |
- |
Lissachatina fulica |
| 3.2.1.B23 | 5 | - |
- |
Lissachatina fulica |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.B23 | 4 | 6.5 | pH 4.0: about 40% of maximal activity, pH 6.5: about 50% of maximal activity | Lissachatina fulica |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 4 | 10 | 30°C, stable for 24 h | Lissachatina fulica |
| 3.2.1.B23 | 4 | 10 | retains more than 82% of its original activity after 24 h at 30°C | Lissachatina fulica |
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