EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.1.1.11 | additional information | the pectinmethylesterase catalyzes pectin de-esterification accelerates by increasing pressure up to 200 MPa in presence of tomato polygalacturonase, higher pressures diminished the tomato pectinmethylesterase activity becoming even lower as compared to atmospheric pressure | Solanum lycopersicum | |
3.2.1.15 | additional information | tomato polygalacturonase activity is influenced indirectly by the presence of active tomato pectinmethylesterase and vice versa during thermal and high-pressure treatment | Solanum lycopersicum |
EC Number | Application | Comment | Organism |
---|---|---|---|
3.2.1.15 | food industry | to preserve or improve rheological properties of tomato based products, the combination of 40°C and 400MPa represents an optimal condition to reduce tomato PG activity while maintaining sufficient pectinmethylesterase activity | Solanum lycopersicum |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.1.1.11 | additional information | the pectinmethylesterase catalyzes pectin de-esterification accelerates by increasing pressure up to 200 MPa in presence of tomato polygalacturonase, higher pressures diminished the tomato pectinmethylesterase activity becoming even lower as compared to atmospheric pressure | Solanum lycopersicum |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.1.11 | pectin + H2O | Solanum lycopersicum | - |
methanol + pectate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.1.11 | Solanum lycopersicum | - |
- |
- |
3.2.1.15 | Solanum lycopersicum | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.1.11 | native enzyme partially, 5.8fold by cation exchange chromatography | Solanum lycopersicum |
3.2.1.15 | 3.02fold | Solanum lycopersicum |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.1.1.11 | fruit | - |
Solanum lycopersicum | - |
3.2.1.15 | fruit | red ripe tomatoes | Solanum lycopersicum | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.1.1.11 | 26.96 | - |
partially purified enzyme, at pH 7.0, 22°C | Solanum lycopersicum |
3.2.1.15 | 5.59 | - |
crude extract | Solanum lycopersicum |
3.2.1.15 | 16.87 | - |
3.02fold purified enzyme | Solanum lycopersicum |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.1.11 | additional information | the pectinmethylesterase catalyzes pectin de-esterification accelerates by increasing pressure up to 200 MPa in presence of tomato polygalacturonase | Solanum lycopersicum | ? | - |
? | |
3.1.1.11 | pectin + H2O | - |
Solanum lycopersicum | methanol + pectate | - |
? | |
3.2.1.15 | pectin + H2O | apple pectin | Solanum lycopersicum | ? | - |
? | |
3.2.1.15 | polygalacturonic acid + H2O | - |
Solanum lycopersicum | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.1.11 | pectinmethylesterase | - |
Solanum lycopersicum |
3.1.1.11 | PME | - |
Solanum lycopersicum |
3.2.1.15 | PG2 | - |
Solanum lycopersicum |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.1.11 | 50 | 60 | at atmospheric pressure to 300 MPa | Solanum lycopersicum |
3.2.1.15 | 50 | - |
optimal temperature for activity of purified tomato PG in presence of highly esterified pectin and purified tomato pectinmethylesterase at atmospheric pressure | Solanum lycopersicum |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.1.11 | 30 | 70 | PME activity during isobaric-isothermal treatment, overview | Solanum lycopersicum |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.1.1.11 | 4.4 | - |
- |
Solanum lycopersicum |
3.2.1.15 | 4.4 | - |
optimal pH for activity of purified tomato PG in presence of highly esterified pectin and purified tomato pectinmethylesterase at atmospheric pressure | Solanum lycopersicum |