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Literature summary extracted from

  • Kunamneni, A.; Singh, S.
    Improved high thermal stability of pullulanase from a newly isolated thermophilic Bacillus sp. AN-7 (2006), Enzyme Microb. Technol., 39, 1399-1404.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.41 dithiothreitol
-
 Bacillus sp. (in: Bacteria)

Application

EC Number Application Comment Organism
3.2.1.41 industry in the starch processing industry for the production of sugar syrups Bacillus sp. (in: Bacteria)

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.41 additional information EDTA has no effect, phenylmethanesulfonyl fluoride and diisopropylflurophosphate have almost no effect Bacillus sp. (in: Bacteria)
3.2.1.41 N-bromosuccinimide inhibitory at 10mM Bacillus sp. (in: Bacteria)

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.41 extracellular
-
 Bacillus sp. (in: Bacteria)
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.41 Ca2+ activating Bacillus sp. (in: Bacteria)
3.2.1.41 Cu2+ partially inactivating Bacillus sp. (in: Bacteria)
3.2.1.41 Hg2+ complete inactivating Bacillus sp. (in: Bacteria)
3.2.1.41 Mn2+ activating Bacillus sp. (in: Bacteria)
3.2.1.41 additional information Fe2+, Mg2+, Ba2+, K+, Na+ or Zn2+ have no effect Bacillus sp. (in: Bacteria)

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.41 106000
-
 SDS-PAGE Bacillus sp. (in: Bacteria)

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.41 Bacillus sp. (in: Bacteria)
-
 from soil in India
-
3.2.1.41 Bacillus sp. (in: Bacteria) AN-7
-
 from soil in India
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.41 ammonium sulfate precipitation, anion exchange and gel filtration chromatography Bacillus sp. (in: Bacteria)

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.41 151.5
-
 after purification Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.41 pullulan + H2O
-
 Bacillus sp. (in: Bacteria) maltotriose + maltose
-
 ?
3.2.1.41 pullulan + H2O
-
 Bacillus sp. (in: Bacteria) AN-7 maltotriose + maltose
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.41 pullulanase
-
 Bacillus sp. (in: Bacteria)
3.2.1.41 pullulanase type I
-
 Bacillus sp. (in: Bacteria)

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.41 90
-
-
 Bacillus sp. (in: Bacteria)

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.41 80 100
-
 Bacillus sp. (in: Bacteria)

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.41 6
-
-
 Bacillus sp. (in: Bacteria)

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.41 5 7
-
 Bacillus sp. (in: Bacteria)