Any feedback?
Literature summary extracted from
- Characteristics of chimeric enzymes constructed between Thermotoga maritima and Agrobacterium tumefaciens beta-glucosidases: role of C-terminal domain in catalytic activity (2006), Enzyme Microb. Technol., 38, 952-959.
No PubMed abstract available
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.2.1.21 | gene bglb, DNA and amino acid sequence determination and anaylsis, expression of N-terminally His-tagged wild-type and chimeric mutant enzymes in Escherichia coli | Thermotoga maritima |
| 3.2.1.21 | gene cbg1, DNA and amino acid sequence determination and anaylsis, expression of N-terminally His-tagged wild-type and chimeric mutant enzymes in Escherichia coli | Agrobacterium tumefaciens |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 3.2.1.21 | additional information | construction of chimeric enzymes between Thermotoga maritima and Agrobacterium tumefaciens beta-glucosidases, three chimeric genes are constructed by shuffling at three selected regions, only two chimeric enzymes are active and show altered properties, e.g. decreased thermal stability, pH optima, and kinetics, overview | Agrobacterium tumefaciens |
| 3.2.1.21 | additional information | construction of chimeric enzymes between Thermotoga maritima and Agrobacterium tumefaciens beta-glucosidases, three chimeric genes are constructed by shuffling at three selected regions, only two chimeric enzymes are active and show altered properties, e.g. decreased thermal stability, pH optima, and kinetics, overview | Thermotoga maritima |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.21 | 0.0039 | - |
4-nitrophenyl-beta-D-glucopyranoside | pH 6.5, 30°C, wild-type enzyme | Thermotoga maritima |  |
| 3.2.1.21 | 0.005 | - |
4-nitrophenyl-beta-D-xylopyranoside | pH 6.5, 30°C, wild-type enzyme | Agrobacterium tumefaciens | |
| 3.2.1.21 | 0.012 | - |
4-nitrophenyl-beta-D-glucopyranoside | pH 6.5, 30°C, wild-type enzyme | Agrobacterium tumefaciens | |
| 3.2.1.21 | 0.08 | - |
4-nitrophenyl-beta-D-fucopyranoside | pH 6.5, 30°C, wild-type enzyme | Agrobacterium tumefaciens | |
| 3.2.1.21 | 0.24 | - |
4-nitrophenyl-alpha-L-arabinopyranoside | pH 6.5, 30°C, wild-type enzyme | Agrobacterium tumefaciens | |
| 3.2.1.21 | 2.64 | - |
4-nitrophenyl-beta-D-xylopyranoside | pH 6.5, 30°C, wild-type enzyme | Thermotoga maritima | |
| 3.2.1.21 | 18.9 | - |
4-nitrophenyl-alpha-L-arabinopyranoside | pH 6.5, 30°C, wild-type enzyme | Thermotoga maritima | |
| 3.2.1.21 | 42.7 | - |
4-nitrophenyl-beta-D-fucopyranoside | pH 6.5, 30°C, wild-type enzyme | Thermotoga maritima | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.21 | Agrobacterium tumefaciens | - |
gene cbg1 | - |
| 3.2.1.21 | Agrobacterium tumefaciens B3/73 | - |
gene cbg1 | - |
| 3.2.1.21 | Thermotoga maritima | - |
gene bglb | - |
| 3.2.1.21 | Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 | - |
gene bglb | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.21 | recombinant N-terminally His-tagged wild-type and chimeric mutant enzymes from Escherichia coli by nickel affinity chromatography | Agrobacterium tumefaciens |
| 3.2.1.21 | recombinant N-terminally His-tagged wild-type and chimeric mutant enzymes from Escherichia coli by nickel affinity chromatography | Thermotoga maritima |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | additional information | - |
substrate specificity of wild-type and chimeric mutant enzymes, overview | Agrobacterium tumefaciens |
| 3.2.1.21 | additional information | - |
substrate specificity of wild-type and chimeric mutant enzymes, overview | Thermotoga maritima |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.21 | 4-nitrophenyl beta-D-fucopyranoside + H2O | - |
Agrobacterium tumefaciens | 4-nitrophenol + beta-D-fucose | - |
? | |
| 3.2.1.21 | 4-nitrophenyl beta-D-fucopyranoside + H2O | - |
Thermotoga maritima | 4-nitrophenol + beta-D-fucose | - |
? | |
| 3.2.1.21 | 4-nitrophenyl beta-D-fucopyranoside + H2O | - |
Agrobacterium tumefaciens B3/73 | 4-nitrophenol + beta-D-fucose | - |
? | |
| 3.2.1.21 | 4-nitrophenyl beta-D-fucopyranoside + H2O | - |
Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 | 4-nitrophenol + beta-D-fucose | - |
? | |
| 3.2.1.21 | 4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Agrobacterium tumefaciens | 4-nitrophenol + beta-D-glucose | - |
? | |
| 3.2.1.21 | 4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Thermotoga maritima | 4-nitrophenol + beta-D-glucose | - |
? | |
| 3.2.1.21 | 4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Agrobacterium tumefaciens B3/73 | 4-nitrophenol + beta-D-glucose | - |
? | |
| 3.2.1.21 | 4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 | 4-nitrophenol + beta-D-glucose | - |
? | |
| 3.2.1.21 | 4-nitrophenyl beta-D-xylopyranoside + H2O | - |
Agrobacterium tumefaciens | 4-nitrophenol + beta-D-xylose | - |
? | |
| 3.2.1.21 | 4-nitrophenyl beta-D-xylopyranoside + H2O | - |
Thermotoga maritima | 4-nitrophenol + beta-D-xylose | - |
? | |
| 3.2.1.21 | 4-nitrophenyl beta-D-xylopyranoside + H2O | - |
Agrobacterium tumefaciens B3/73 | 4-nitrophenol + beta-D-xylose | - |
? | |
| 3.2.1.21 | 4-nitrophenyl beta-D-xylopyranoside + H2O | - |
Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 | 4-nitrophenol + beta-D-xylose | - |
? | |
| 3.2.1.21 | 4-nitrophenyl-alpha-L-arabinopyranoside + H2O | - |
Agrobacterium tumefaciens | 4-nitrophenol + alpha-L-arabinose | - |
? | |
| 3.2.1.21 | 4-nitrophenyl-alpha-L-arabinopyranoside + H2O | - |
Thermotoga maritima | 4-nitrophenol + alpha-L-arabinose | - |
? | |
| 3.2.1.21 | 4-nitrophenyl-alpha-L-arabinopyranoside + H2O | - |
Agrobacterium tumefaciens B3/73 | 4-nitrophenol + alpha-L-arabinose | - |
? | |
| 3.2.1.21 | 4-nitrophenyl-alpha-L-arabinopyranoside + H2O | - |
Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 | 4-nitrophenol + alpha-L-arabinose | - |
? | |
| 3.2.1.21 | additional information | substrate specificity of wild-type and chimeric mutant enzymes, overview | Agrobacterium tumefaciens | ? | - |
? | |
| 3.2.1.21 | additional information | substrate specificity of wild-type and chimeric mutant enzymes, overview | Thermotoga maritima | ? | - |
? | |
| 3.2.1.21 | additional information | substrate specificity of wild-type and chimeric mutant enzymes, overview | Agrobacterium tumefaciens B3/73 | ? | - |
? | |
| 3.2.1.21 | additional information | substrate specificity of wild-type and chimeric mutant enzymes, overview | Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.1.21 | More | the enzyme belongs to the glycosyl hydrolase family 3 | Agrobacterium tumefaciens |
| 3.2.1.21 | More | the enzyme belongs to the glycosyl hydrolase family 3 | Thermotoga maritima |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 65 | - |
- |
Agrobacterium tumefaciens |
| 3.2.1.21 | 85 | - |
- |
Thermotoga maritima |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.21 | 6.4 | - |
4-nitrophenyl-beta-D-glucopyranoside | pH 6.5, 30°C, wild-type enzyme | Thermotoga maritima | |
| 3.2.1.21 | 9 | - |
4-nitrophenyl-alpha-L-arabinopyranoside | pH 6.5, 30°C, wild-type enzyme | Thermotoga maritima | |
| 3.2.1.21 | 18.4 | - |
4-nitrophenyl-beta-D-xylopyranoside | pH 6.5, 30°C, wild-type enzyme | Thermotoga maritima | |
| 3.2.1.21 | 22.1 | - |
4-nitrophenyl-beta-D-fucopyranoside | pH 6.5, 30°C, wild-type enzyme | Agrobacterium tumefaciens | |
| 3.2.1.21 | 27.7 | - |
4-nitrophenyl-beta-D-fucopyranoside | pH 6.5, 30°C, wild-type enzyme | Thermotoga maritima | |
| 3.2.1.21 | 28.9 | - |
4-nitrophenyl-beta-D-xylopyranoside | pH 6.5, 30°C, wild-type enzyme | Agrobacterium tumefaciens | |
| 3.2.1.21 | 95.4 | - |
4-nitrophenyl-beta-D-glucopyranoside | pH 6.5, 30°C, wild-type enzyme | Agrobacterium tumefaciens | |
| 3.2.1.21 | 119 | - |
4-nitrophenyl-alpha-L-arabinopyranoside | pH 6.5, 30°C, wild-type enzyme | Agrobacterium tumefaciens | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 3.2 | 3.5 | - |
Thermotoga maritima |
| 3.2.1.21 | 6.5 | 8 | - |
Agrobacterium tumefaciens |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 3.5 | 11 | - |
Thermotoga maritima |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
