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Literature summary extracted from

  • Beassoni, P.R.; Otero, L.H.; Massimelli, M.J.; Lisa, A.T.; Domenech, C.E.
    Critical active-site residues identified by site-directed mutagenesis in Pseudomonas aeruginosa phosphorylcholine phosphatase, a new member of the haloacid dehalogenases hydrolase superfamily (2006), Curr. Microbiol., 53, 534-539.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.3.75 additional information signal peptide alters the low-affinity site of recombinant PChP expressed in Escherichia coli Pseudomonas aeruginosa

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.75 overexpression of the wild-type and mutant enzymes in Escherichia coli ER2566, transformed with pTY-pchP327, pTYpchP349, or the mutated expression plasmids Pseudomonas aeruginosa

Protein Variants

EC Number Protein Variants Comment Organism
3.1.3.75 D262E low PChP activity Pseudomonas aeruginosa
3.1.3.75 D265E enzyme activity reduced to 30-35% of wild-type activity Pseudomonas aeruginosa
3.1.3.75 D267E low PChP activity Pseudomonas aeruginosa
3.1.3.75 D31E protein expression, but no enzyme activity Pseudomonas aeruginosa
3.1.3.75 D33E protein expression, but no enzyme activity Pseudomonas aeruginosa
3.1.3.75 G261A reduced activity as compared to wild-type Pseudomonas aeruginosa
3.1.3.75 K242Q is inactive Pseudomonas aeruginosa
3.1.3.75 K242R functional enzyme Pseudomonas aeruginosa
3.1.3.75 S166T reduced PChP activity Pseudomonas aeruginosa
3.1.3.75 T35S protein expression, low PChP activity Pseudomonas aeruginosa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.75 0.03
-
 phosphocholine at pH 5 and pH 7.4, without signal peptide Pseudomonas aeruginosa
3.1.3.75 0.03
-
 phosphocholine mutant T35S Pseudomonas aeruginosa
3.1.3.75 0.05
-
 phosphocholine at pH 5 and pH 7.4, with signal peptide Pseudomonas aeruginosa
3.1.3.75 0.5
-
 phosphocholine at pH 5 and pH 7.4, without signal peptide Pseudomonas aeruginosa
3.1.3.75 0.5
-
 phosphocholine mutant T35S Pseudomonas aeruginosa
3.1.3.75 2
-
 p-nitrophenyl phosphate at pH 5, with and without signal peptide Pseudomonas aeruginosa
3.1.3.75 2.1
-
 p-nitrophenyl phosphate mutant T35S Pseudomonas aeruginosa
3.1.3.75 3.5
-
 phosphocholine at pH 7.4, with signal peptide Pseudomonas aeruginosa
3.1.3.75 3.6
-
 phosphocholine at pH 5, with signal peptide Pseudomonas aeruginosa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.75 Mg2+
-
 Pseudomonas aeruginosa

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.75 Pseudomonas aeruginosa
-
 PAO1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.75
-
 Pseudomonas aeruginosa

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.75 1.3
-
 mutant D262E, at pH 5, with p-nitrophenyl phosphate as substrate Pseudomonas aeruginosa
3.1.3.75 1.5
-
 mutant D267E, at pH 5, with p-nitrophenyl phosphate as substrate Pseudomonas aeruginosa
3.1.3.75 2.4
-
 mutant D267E, at pH 5, with phosphocholine as substrate Pseudomonas aeruginosa
3.1.3.75 2.5
-
 mutant D267E, at pH 7.4, with phosphocholine as substrate Pseudomonas aeruginosa
3.1.3.75 3
-
 mutant T35S, at pH 5, with phosphocholine as substrate Pseudomonas aeruginosa
3.1.3.75 3.6
-
 mutant T35S, at pH 7.4, with phosphocholine as substrate Pseudomonas aeruginosa
3.1.3.75 5.9
-
 mutant T35S, at pH 5, with p-nitrophenyl phosphate as substrate Pseudomonas aeruginosa
3.1.3.75 6.5
-
 mutant D262E, at pH 5, with phosphocholine as substrate Pseudomonas aeruginosa
3.1.3.75 7
-
 mutant S166T, at pH 5, with p-nitrophenyl phosphate as substrate Pseudomonas aeruginosa
3.1.3.75 11.1
-
 mutant D262E, at pH 7.4, with phosphocholine as substrate Pseudomonas aeruginosa
3.1.3.75 20.3
-
 mutant S166T, at pH 5, with phosphocholine as substrate Pseudomonas aeruginosa
3.1.3.75 26.2
-
 mutant S166T, at pH 7.4, with phosphocholine as substrate Pseudomonas aeruginosa
3.1.3.75 38.8
-
 mutant G261A, at pH 5, with phosphocholine as substrate Pseudomonas aeruginosa
3.1.3.75 56.9
-
 mutant K242R, at pH 5, with phosphocholine as substrate Pseudomonas aeruginosa
3.1.3.75 63.4
-
 mutant G261A, at pH 5, with p-nitrophenyl phosphate as substrate Pseudomonas aeruginosa
3.1.3.75 64
-
 mutant D265E, at pH 5, with p-nitrophenyl phosphate as substrate Pseudomonas aeruginosa
3.1.3.75 64.8
-
 mutant G261A, at pH 7.4, with phosphocholine as substrate Pseudomonas aeruginosa
3.1.3.75 70
-
 mutant D265E, at pH 7.4, with phosphocholine as substrate Pseudomonas aeruginosa
3.1.3.75 71
-
 mutant D265E, at pH 5, with phosphocholine as substrate Pseudomonas aeruginosa
3.1.3.75 74.5
-
 wild-type with motif 31DMDNT35, at pH 5, with p-nitrophenyl phosphate as substrate Pseudomonas aeruginosa
3.1.3.75 74.6
-
 mutant K242R, at pH 7.4, with phosphocholine as substrate Pseudomonas aeruginosa
3.1.3.75 86.9
-
 wild-type with motif 31DMDNT35, at pH 7.4, with phosphocholine as substrate Pseudomonas aeruginosa
3.1.3.75 88.4
-
 wild-type with motif 31DMDNT35, at pH 5, with phosphocholine as substrate Pseudomonas aeruginosa
3.1.3.75 100
-
 wild-type with motif 166S, motif 242K or motif 161GDTPDSD267, at pH 5, with p-nitrophenyl phosphate as substrate and at pH 5 and pH 7.4 with phosphocholine as substrate Pseudomonas aeruginosa
3.1.3.75 106.7
-
 mutant K242R, at pH 5, with p-nitrophenyl phosphate as substrate Pseudomonas aeruginosa

Storage Stability

EC Number Storage Stability Organism
3.1.3.75 -10°C, 10 mM Tris-HCl, pH 8, 30% glycerol Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.75 p-nitrophenyl phosphate + H2O
-
 Pseudomonas aeruginosa 4-nitrophenol + phosphate
-
 ?
3.1.3.75 phosphocholine + H2O D31, D33, D262, D267, S166 or K242 are important residues for catalysis Pseudomonas aeruginosa choline + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.3.75 PChP
-
 Pseudomonas aeruginosa
3.1.3.75 phosphorylcholine phosphatase
-
 Pseudomonas aeruginosa