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Literature summary extracted from

  • Mohamed, M.A.; Abdel-Gawad, A.S.; Ghazy, A.E.
    Purification and characterization of an acetylcholinesterase from the infective juveniles of Heterorhabditis bacteriophora (2007), Comp. Biochem. Physiol. C, 146, 314-324.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.7 BW284c51 isozyme AChE1A, complete competitive inhibition at 0.016 mM Heterorhabditis bacteriophora
3.1.1.7 eserine isozyme AChE1A, complete competitive inhibition at 0.016 mM Heterorhabditis bacteriophora
3.1.1.7 additional information no inhibition by iso-OMPA Heterorhabditis bacteriophora

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.7 additional information
-
 additional information MIchaelis-Menten kinetics, activation energy of 9 kcal/mol Heterorhabditis bacteriophora
3.1.1.7 0.27
-
 acetylthiocholine iodide pH 7.5, 37°C, isozyme AChE1A Heterorhabditis bacteriophora
3.1.1.7 0.42
-
 propionylthiocholine iodide pH 7.5, 37°C, isozyme AChE1A Heterorhabditis bacteriophora
3.1.1.7 0.59
-
 butyrylthiocholine iodide pH 7.5, 37°C, isozyme AChE1A Heterorhabditis bacteriophora

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.7 Heterorhabditis bacteriophora
-
 eight different Egyptian strains EA2, EB6, EB7, EG7, EG8, EG9, EK1 and EK2, different isozymes, i.e. AChE1A, isozyme pattern
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.7 isozyme AChE1A from strain EG9, 17.5fold from infective juveniles, by ammonium sulfate fractionation, gel filtration, and anion exchange chromatography to homogeneity Heterorhabditis bacteriophora

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.7 juvenile infective Heterorhabditis bacteriophora
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.7 34
-
 strain EG7 Heterorhabditis bacteriophora
3.1.1.7 44.2
-
 strain EB7 Heterorhabditis bacteriophora
3.1.1.7 59.1
-
 strain EA2 Heterorhabditis bacteriophora
3.1.1.7 59.9
-
 strain EK2 and EK1 Heterorhabditis bacteriophora
3.1.1.7 60.1
-
 strain EG8 Heterorhabditis bacteriophora
3.1.1.7 71.5
-
 strain EB6 Heterorhabditis bacteriophora
3.1.1.7 82.6
-
 strain EG9 Heterorhabditis bacteriophora
3.1.1.7 1378
-
 purified isozyme AChE1A from strain EG9 Heterorhabditis bacteriophora

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.7 acetylthiocholine iodide + H2O preferred substrate Heterorhabditis bacteriophora thiocholine iodide + acetate
-
 ?
3.1.1.7 benzoylthiocholine iodide + H2O 22.2% activity compared to acetylthiocholine Heterorhabditis bacteriophora thiocholine iodide + benzoate
-
 ?
3.1.1.7 butyrylthiocholine iodide + H2O 41.7% activity compared to acetylthiocholine Heterorhabditis bacteriophora thiocholine iodide + butyrate
-
 ?
3.1.1.7 propionylthiocholine iodide + H2O 74.6% activity compared to acetylthiocholine Heterorhabditis bacteriophora thiocholine iodide + propionate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.7 AChE
-
 Heterorhabditis bacteriophora
3.1.1.7 AChE1A
-
 Heterorhabditis bacteriophora
3.1.1.7 ChE1A
-
 Heterorhabditis bacteriophora

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.7 35
-
-
 Heterorhabditis bacteriophora

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.7 50
-
 purified isozyme AChE1A, irreversible inactivation above Heterorhabditis bacteriophora
3.1.1.7 60
-
 30 min, purified isozyme AChE1A, loss of 46% activity Heterorhabditis bacteriophora
3.1.1.7 70
-
 30 min, purified isozyme AChE1A, loss of 63% activity Heterorhabditis bacteriophora
3.1.1.7 80
-
 30 min, purified isozyme AChE1A, loss of 82% activity Heterorhabditis bacteriophora

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.7 7.5
-
-
 Heterorhabditis bacteriophora

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.1.1.7 0.0045
-
 BW284c51 pH 7.5, 37°C, isozyme AChE1A Heterorhabditis bacteriophora
3.1.1.7 0.005
-
 eserine pH 7.5, 37°C, isozyme AChE1A Heterorhabditis bacteriophora

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.1.1.7 0.003
-
 pH 7.5, 37°C, isozyme AChE1A Heterorhabditis bacteriophora BW284c51
3.1.1.7 0.0035
-
 pH 7.5, 37°C, isozyme AChE1A Heterorhabditis bacteriophora eserine