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Literature summary extracted from
- Structure of Saccharomyces cerevisiae chitinase 1 and screening-based discovery of potent inhibitors (2007), Chem. Biol., 14, 589-599.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.2.1.14 | expression of the catalytic domain of ScCTS1 (comprising of amino acids 22 to 315) as a secreted N-terminally His-tagged fusion protein in Pichia pastoris | Saccharomyces cerevisiae |
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 3.2.1.14 | crystallization of the native enzyme and the enzyme in complex with 8-chlorotheophylline, kinetin or acetazolamide, sitting drop method, 1.6 A resolution | Saccharomyces cerevisiae |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 3.2.1.14 | A283S | Km-value for mutant enzyme for 4-methylumbelliferyl-N,N',N''-triacetylchitotriose is 0.8 mM versus 1 mM for wild-type enzyme and turnover number is 2.3 per sec versus 1.3 for wild-type enzyme. Inhibition by allosamidin, acetazolamide, and 8-chlorotheophylline are not significantly affected by the mutation, inhibition by kinetin is not detectable | Saccharomyces cerevisiae |
| 3.2.1.14 | F210W/A283S | similar Km (1.1 versus 1 mM for wild-type) and turnover number kcat (0.8 versus 1.3 for wild-type) as for the wild-type enzyme and the single A283S mutant. Inhibition by allosamidin, acetazolamide, and 8-chlorotheophylline is not significantly affected by the mutation, inhibition by kinetin is not detectable | Saccharomyces cerevisiae |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.14 | 8-chlorotheophylline | - |
Saccharomyces cerevisiae |  |
| 3.2.1.14 | acetazolamide | competitive | Saccharomyces cerevisiae | |
| 3.2.1.14 | allosamidin | - |
Saccharomyces cerevisiae | |
| 3.2.1.14 | kinetin | competitive | Saccharomyces cerevisiae | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.14 | 0.8 | - |
4-methylumbelliferyl-N,N',N''-triacetylchitotriose | mutant enzyme A283S | Saccharomyces cerevisiae | |
| 3.2.1.14 | 1 | - |
4-methylumbelliferyl-N,N',N''-triacetylchitotriose | wild-type enzyme | Saccharomyces cerevisiae | |
| 3.2.1.14 | 1.1 | - |
4-methylumbelliferyl-N,N',N''-triacetylchitotriose | mutant enzyme F210W/A283S | Saccharomyces cerevisiae | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.14 | Saccharomyces cerevisiae | P29029 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.14 | - |
Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.14 | 4-methylumbelliferyl-N,N',N''-triacetylchitotriose + H2O | - |
Saccharomyces cerevisiae | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.1.14 | chitinase 1 | - |
Saccharomyces cerevisiae |
| 3.2.1.14 | CTS1 | - |
Saccharomyces cerevisiae |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.14 | 0.8 | - |
4-methylumbelliferyl-N,N',N''-triacetylchitotriose | mutant enzyme F210W/A283S | Saccharomyces cerevisiae | |
| 3.2.1.14 | 1.3 | - |
4-methylumbelliferyl-N,N',N''-triacetylchitotriose | wild-type enzyme | Saccharomyces cerevisiae | |
| 3.2.1.14 | 2.3 | - |
4-methylumbelliferyl-N,N',N''-triacetylchitotriose | mutant enzyme A283S | Saccharomyces cerevisiae | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.14 | 2.6 | - |
substrate: 4-methylumbelliferyl-N,N',N''-triacetylchitotriose | Saccharomyces cerevisiae |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.14 | 2 | 4 | pH 2: about 80% of maximal activity, pH 4.0: about 55% of maximal activity, substrate: 4-methylumbelliferyl-N,N',N''-triacetylchitotriose | Saccharomyces cerevisiae |
Ki Value [mM]
| EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.14 | 0.00061 | - |
allosamidin | wild-type enzyme | Saccharomyces cerevisiae | |
| 3.2.1.14 | 0.00066 | - |
allosamidin | mutant enzyme A283S | Saccharomyces cerevisiae | |
| 3.2.1.14 | 0.00146 | - |
allosamidin | mutant enzyme F210W/A283S | Saccharomyces cerevisiae | |
| 3.2.1.14 | 0.0032 | - |
kinetin | wild-type enzyme | Saccharomyces cerevisiae | |
| 3.2.1.14 | 0.021 | - |
acetazolamide | wild-type enzyme | Saccharomyces cerevisiae | |
| 3.2.1.14 | 0.071 | - |
acetazolamide | mutant enzyme A283S | Saccharomyces cerevisiae | |
| 3.2.1.14 | 0.128 | - |
acetazolamide | mutant enzyme F210W/A283S | Saccharomyces cerevisiae | |
| 3.2.1.14 | 0.6 | - |
8-chlorotheophylline | wild-type enzyme | Saccharomyces cerevisiae | |
| 3.2.1.14 | 1.155 | - |
8-chlorotheophylline | mutant enzyme A283S | Saccharomyces cerevisiae | |
| 3.2.1.14 | 1.192 | - |
8-chlorotheophilline | mutant enzyme F210W/A283S | Saccharomyces cerevisiae | |
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