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Literature summary extracted from
- Autotaxin (NPP-2) in the brain: cell type-specific expression and regulation during development and after neurotrauma (2007), Cell. Mol. Life Sci., 64, 230-243.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.1.4.39 | COS-7 cells (ATCC: CRL-1651) and HEK 293 cells. To gain further insight into the possible functional role of ATX in the brain, analysis of its cellular expression in the brain under physiological conditions and following trauma. Preparation and purification of oligodendrocyte precursor cells: Primary mixed glial cultures are prepared from the cerebral hemispheres of P0P2 Sprague-Dawley rat pups from which the meninges has been stripped | Rattus norvegicus |
| 3.1.4.39 | expressed in COS-7 cells (ATCC: CRL-1651) and HEK 293 cells. Compared distribution of overexpressed ATX in COS-7 and HEK293 cells | Mus musculus |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.1.4.39 | extracellular | - |
Mus musculus | - |
- |
| 3.1.4.39 | extracellular | - |
Rattus norvegicus | - |
- |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.4.39 | 125000 | - |
extracellular enzyme which is originally identified as an autocrine tumor cell motility stimulating factor overexpressed in a variety of human cancers including primary brain tumors | Rattus norvegicus |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.4.39 | lysophosphatidylcholine + H2O | Mus musculus | - |
lysophosphatidic acid + choline | - |
? |  |
| 3.1.4.39 | lysophosphatidylcholine + H2O | Rattus norvegicus | - |
lysophosphatidic acid + choline | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.4.39 | Mus musculus | - |
- |
- |
| 3.1.4.39 | Rattus norvegicus | - |
Sprague-Dawley rat | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.4.39 | using GSH-Sepharose | Rattus norvegicus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.1.4.39 | brain | developmental regulation of autotaxin shown by mRNA expression in the brain, analyzed by quanitative RT-PCR, using recombined ATX-green fluorescent protein (GFP), immunocytochemistry visualized with 3,3'-diaminobenzidine as a chromogen, for double-labeling immunofluorescence several markers used | Mus musculus | - |
| 3.1.4.39 | brain | developmental regulation of auzotaxin shown by mRNA expression in the brain, analyzed by quanitative RT-PCR, using recombined ATX-green fluorescent protein (GFP), immunocytochemistry visualized with 3,3'-diaminobenzidine as a chromogen, for double-labeling immunofluorescence several markers used | Rattus norvegicus | - |
| 3.1.4.39 | additional information | secreted cell motility-stimulating exo-phosphodiesterase with lysophospholipase D activity. Distribution of autotaxin in the developing and lesioned brain, function of autotaxin in oligodendrocyte precursor cells and brain injuries. Visual localization of NPP-2 in the brain. Developmental regulation of ATX. Cellular and subcellular distribution of ATX shown. These data indicate that ATX expression commences prior to the onset of myelin protein expression and myelination in the brain. Developmental regulation of autotaxin (ATX) mRNA expression in the brain. ATX is expressed in early and late oligodendrocyte precursor cells (OPCs). ATX is up-regulated and cell-type-specifically expressed following neurotrauma | Mus musculus | - |
| 3.1.4.39 | additional information | secreted cell motility-stimulating exo-phosphodiesterase with lysophospholipase D activity. Distribution of autotaxin in the developing and lesioned brain, function of autotaxin in oligodendrocyte precursor cells and brain injuries. Visual localization of NPP-2 in the brain. Developmental regulation of ATX. Cellular and subcellular distribution of ATX shown. These data indicate that ATX expression commences prior to the onset of myelin protein expression and myelination in the brain. Developmental regulation of autotaxin (ATX) mRNA expression in the brain. ATX is expressed in early and late oligodendrocyte precursor cells (OPCs). ATX is up-regulated and cell-type-specifically expressed following neurotrauma | Rattus norvegicus | - |
| 3.1.4.39 | subventricular zone | during embryonic development. In vivo expression of autotaxin in the brain during development and following neurotrauma | Mus musculus | - |
| 3.1.4.39 | subventricular zone | during embryonic development. In vivo expression of autotaxin in the brain during development and following neurotrauma | Rattus norvegicus | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.4.39 | lysophosphatidylcholine + H2O | - |
Mus musculus | lysophosphatidic acid + choline | - |
? | |
| 3.1.4.39 | lysophosphatidylcholine + H2O | - |
Rattus norvegicus | lysophosphatidic acid + choline | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.4.39 | autotaxin | - |
Mus musculus |
| 3.1.4.39 | autotaxin | - |
Rattus norvegicus |
| 3.1.4.39 | lysophospholipase D | - |
Mus musculus |
| 3.1.4.39 | lysophospholipase D | - |
Rattus norvegicus |
| 3.1.4.39 | NPP-2 | - |
Mus musculus |
| 3.1.4.39 | NPP-2 | - |
Rattus norvegicus |
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