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Literature summary extracted from

  • Juarez, A.B.; Aldonatti, C.; Vigna, M.S.; Rios de Molina, M.d.e.l.C.
    Studies on uroporphyrinogen decarboxylase from Chlorella kessleri (Trebouxiophyceae, Chlorophyta) (2007), Can. J. Microbiol., 53, 303-312.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.37 0.0032
-
 uroporphyrinogen III
-
 Parachlorella kessleri

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.37 60000
-
 SDS-PAGE, monomer Parachlorella kessleri
4.1.1.37 90000
-
 SDS-PAGE, active dimeric form Parachlorella kessleri

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.37 Parachlorella kessleri
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.37 partial, Superdex 75 gel filtration Parachlorella kessleri

Reaction

EC Number Reaction Comment Organism Reaction ID
4.1.1.37 uroporphyrinogen III = coproporphyrinogen III + 4 CO2 the decarboxylation of uroporphyrinogen occurs in two stages, via 7 COOH and then 6 and 5 COOH intermediates, with the decarboxylation of the 7 COOH compound being the rate-limiting step for the reaction Parachlorella kessleri
a

Storage Stability

EC Number Storage Stability Organism
4.1.1.37 -20°C, in 30-70% ammonium saturation fraction, 2 weeks, remains stable Parachlorella kessleri
4.1.1.37 -20°C, in 30-70% ammonium saturation fraction, 8 weeks, 50% loss of activity Parachlorella kessleri
4.1.1.37 -20°C, unprecipitated protein, 2 weeks, 36% loss of activity Parachlorella kessleri

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.37 Uroporphyrinogen III
-
 Parachlorella kessleri Coproporphyrinogen III + 4 CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.1.37 dimer gel filtration Parachlorella kessleri

Synonyms

EC Number Synonyms Comment Organism
4.1.1.37 UroD
-
 Parachlorella kessleri

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.1.1.37 45
-
 activity increases up to 45°C Parachlorella kessleri
4.1.1.37 65
-
 activity decreases sharply at 65°C Parachlorella kessleri

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.1.37 6 6.5
-
 Parachlorella kessleri