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Literature summary extracted from

  • Celestino, K.R.; Cunha, R.B.; Felix, C.R.
    Characterization of a beta-glucanase produced by Rhizopus microsporus var. microsporus, and its potential for application in the brewing industry (2006), BMC Biochem., 7, 23.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
3.2.1.73 nutrition the enzyme is able to reduce both the viscosity of the brewer mash and the filtration time, indicating its potential value for the brewing industry Rhizopus microsporus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.73 Cu2+ 0.3% residual activity at 12 mM Rhizopus microsporus
3.2.1.73 Fe3+ 89.6% residual activity at 12 mM Rhizopus microsporus
3.2.1.73 Mg2+ 95.2% residual activity at 12 mM Rhizopus microsporus
3.2.1.73 Mn2+ 62.3% residual activity at 12 mM Rhizopus microsporus
3.2.1.73 additional information shows no sensitivity to Ca2+ and Al3+ Rhizopus microsporus
3.2.1.73 Zn2+ 65% residual activity at 12 mM Rhizopus microsporus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.73 33730
-
 mass spectrometry Rhizopus microsporus
3.2.1.73 36500
-
 SDS-PAGE Rhizopus microsporus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.73 Rhizopus microsporus
-
 variant microsporus
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.73 SP-Sepharose column chromatography and Sephacryl S-100 gel filtration Rhizopus microsporus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.73 0.197
-
 concentrated crude extract, at 50°C, pH 5 Rhizopus microsporus
3.2.1.73 12.6
-
 after 55.53fold purification, at 50°C, pH 5 Rhizopus microsporus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.73 1,3-1,4-beta-glucan + H2O
-
 Rhizopus microsporus ?
-
 ?
3.2.1.73 lichenan + H2O
-
 Rhizopus microsporus ?
-
 ?
3.2.1.73 additional information the enzyme shows only residual activity against 1,3-beta-glucan (laminarin) or no activity at all against 1,4-beta-glucan (cellulose), chitin, xylan, or manan Rhizopus microsporus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.73 1,3-1,4-beta-glucanase
-
 Rhizopus microsporus
3.2.1.73 beta-glucanase
-
 Rhizopus microsporus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.73 50 60
-
 Rhizopus microsporus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.73 50 70 after 2 h incubation at 50°C and 60°C, the residual activity remaines 100% and 50%, respectively, while the enzymatic activity is abolished after 3 min incubation at 70°C Rhizopus microsporus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.73 4 5
-
 Rhizopus microsporus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.73 6
-
 no enzyme activity is detected at pH higher than 6 Rhizopus microsporus