EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.3.2.10 | Tween-80 | when the cosolvent DMSO is replaced by an emulsifier (Tween-80, 0.5% w/v) as an alternative additive to help disperse the water-insoluble substrate, the apparent activity of the epoxide hydrolase significantly increased by about 1.8fold | Priestia megaterium |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.3.2.10 | acetone | - |
Priestia megaterium | |
3.3.2.10 | Ag+ | 5 mM, more than 90% inhibition | Priestia megaterium | |
3.3.2.10 | Al3+ | 5 mM, more than 90% inhibition | Priestia megaterium | |
3.3.2.10 | Cu2+ | 5 mM, more than 90% inhibition | Priestia megaterium | |
3.3.2.10 | Fe2+ | 5 mM, more than 90% inhibition | Priestia megaterium | |
3.3.2.10 | Fe3+ | 5 mM, more than 90% inhibition | Priestia megaterium | |
3.3.2.10 | Ni2+ | 5 mM, more than 90% inhibition | Priestia megaterium | |
3.3.2.10 | tetrahydrofuran | - |
Priestia megaterium | |
3.3.2.10 | Zn2+ | 5 mM, more than 90% inhibition | Priestia megaterium |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.3.2.10 | Ca2+ | 5 mM, activity is enhanced to 108% of control | Priestia megaterium | |
3.3.2.10 | Mg2+ | 5 mM, activity is enhanced to 112% of control | Priestia megaterium | |
3.3.2.10 | Mn2+ | 5 mM, activity is enhanced to 110% of control | Priestia megaterium |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.3.2.10 | Priestia megaterium | - |
- |
- |
3.3.2.10 | Priestia megaterium ECU1001 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.3.2.10 | partial | Priestia megaterium |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.3.2.10 | (R)-glycidyl phenyl ether + H2O | the hydrolysis of (R)-epoxide in the presence of Tween-80 is 25.6 times faster than that of the (S)-epoxide | Priestia megaterium | ? | - |
? | |
3.3.2.10 | (R)-glycidyl phenyl ether + H2O | the hydrolysis of (R)-epoxide in the presence of Tween-80 is 25.6 times faster than that of the (S)-epoxide | Priestia megaterium ECU1001 | ? | - |
? | |
3.3.2.10 | 2-methylphenyl glycidyl ether + H2O | - |
Priestia megaterium | ? | - |
? | |
3.3.2.10 | 2-methylphenyl glycidyl ether + H2O | - |
Priestia megaterium ECU1001 | ? | - |
? | |
3.3.2.10 | 4-methylphenylglycidyl ether + H2O | - |
Priestia megaterium | ? | - |
? | |
3.3.2.10 | 4-methylphenylglycidyl ether + H2O | - |
Priestia megaterium ECU1001 | ? | - |
? |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.3.2.10 | 30 | - |
- |
Priestia megaterium |
3.3.2.10 | 40 | - |
when the cosolvent DMSO is replaced by an emulsifier (Tween-80, 0.5% w/v) as an alternative additive to help disperse the water-insoluble substrate the temperature optimum shifts from 30°C to 40°C | Priestia megaterium |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.3.2.10 | 30 | - |
1 h, no loss of activity | Priestia megaterium |
3.3.2.10 | 40 | - |
6 h, stable | Priestia megaterium |
3.3.2.10 | 50 | - |
when the cosolvent DMSO is replaced by an emulsifier (Tween-80, 0.5% w/v) as an alternative additive to help disperse the water-insoluble substrate the half-life of the enzyme at 50°C increases by 2.5times | Priestia megaterium |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.3.2.10 | 6.5 | - |
- |
Priestia megaterium |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.3.2.10 | 6.5 | 7.5 | quite stable at | Priestia megaterium |