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Literature summary extracted from

  • Basu, A.; Chaudhuri, P.; Malakar, D.; Ghosh, A.K.
    Co-purification of glucanase with acid trehalase-invertase aggregate in Saccharomyces cerevisiae (2008), Biotechnol. Lett., 30, 299-304.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.39 additional information an enzyme deletion strain shows decreased activity of acid trehalase Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.39 additional information Saccharomyces cerevisiae role as a regulator for the acid trehalase activity by association in the enzyme aggregate ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.26 Saccharomyces cerevisiae
-
-
-
3.2.1.28 Saccharomyces cerevisiae
-
-
-
3.2.1.39 Saccharomyces cerevisiae
-
 isoform Bgl2
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.26 ammonium sulfate fractionation, DEAE-Sephadex-A50 chromatography, and HPLC gel filtration on Protein Pak 300 SW Saccharomyces cerevisiae
3.2.1.28 ammonium sulfate precipitation, DEAE-Sephadex-A50 column chromatography, and HPLC gel filtration on Protein Pak 300 SW Saccharomyces cerevisiae
3.2.1.39 isoform Bgl2 copurifies with acid trehalase Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.26 0.02
-
 soluble cell extract, in 100 mM sodium citrate buffer (pH 4.5) Saccharomyces cerevisiae
3.2.1.26 12.3
-
 after purification, in 100 mM sodium citrate buffer (pH 4.5) Saccharomyces cerevisiae
3.2.1.39 3.5
-
 pH 4.5, 37°C Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.26 sucrose + H2O
-
 Saccharomyces cerevisiae D-glucose + D-fructose
-
 ?
3.2.1.28 trehalose + H2O
-
 Saccharomyces cerevisiae D-glucose
-
 ?
3.2.1.39 laminarin + H2O
-
 Saccharomyces cerevisiae ?
-
 ?
3.2.1.39 additional information role as a regulator for the acid trehalase activity by association in the enzyme aggregate Saccharomyces cerevisiae ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.39 ? x * 37000-41000, SDS-PAGE Saccharomyces cerevisiae

Synonyms

EC Number Synonyms Comment Organism
3.2.1.26 invertase
-
 Saccharomyces cerevisiae
3.2.1.28 acid trehalase
-
 Saccharomyces cerevisiae
3.2.1.39 Bgl2 isoform Saccharomyces cerevisiae