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Literature summary extracted from

  • Li, X.; Millson, S.H.; Coker, R.D.; Evans, I.H.
    Cloning and expression of Penicillium minioluteum dextranase in Saccharomyces cerevisiae and its exploitation as a reporter in the detection of mycotoxins (2006), Biotechnol. Lett., 28, 1955-1964.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.11 expressed in Saccharomyces cerevisiae INVSc1 via fusion of the DNA segment encoding the mature dextranase protein with alpha-factor signal sequence, and insertion into the GAL1-controlled expression vector pYES2/CT (construction of the four dextranase-expressing plasmids pYES2/CT-dex, pYES2/CT-dex-tag, pYES2/CT-alphaF-dex(m) and pYES2/CT-alphaF-dex(m)-tag). Dextranase construct pYESCT2-alphaF-dex(m) introduced into a strain of Saccharomyces cerevisiae expressing the human cytochrome P4503A4 (CYP3A4) and the cognate reductase Talaromyces minioluteus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.11 aflatoxin B1 inhibits dextranase activities of CYP3A4-expressing cultures, maximum inhibition of 22% at 2 microg/ml. Only negligibly inhibits strain INVSC1 expressing dextranase Talaromyces minioluteus
3.2.1.11 aflatoxin G1 substantially less inhibitory towards dextranase activities of CYP3A4-expressing cultures than aflatoxin B1, showing a maximum inhibition of about 12% at 2 microg/ml, whereas strain INVSC1 expressing dextranase is only marginally inhibited Talaromyces minioluteus
3.2.1.11 additional information no significant inhibition of dextranase activities of CYP3A4-expressing cultures and strain INVSC1 at concentrations of 2, 20, 200, and 2000 ng/ml by deoxynivalenol, fumonisin B1, and ochratoxin A Talaromyces minioluteus
3.2.1.11 T-2 toxin 100% inhibition at 10 microg/ml of dextranase activities of CYP3A4-expressing cultures and strain INVSC1 Talaromyces minioluteus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.11 Talaromyces minioluteus Q27JJ3
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3.2.1.11 Talaromyces minioluteus IMI068219 Q27JJ3
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.11 (4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-sindacene-3-propionic acid, succinimidyl ester)-quenched dextran + H2O
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 Talaromyces minioluteus ?
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 ?
3.2.1.11 (4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-sindacene-3-propionic acid, succinimidyl ester)-quenched dextran + H2O
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 Talaromyces minioluteus IMI068219 ?
-
 ?